Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of alkalophilic bacillus ntt33c6 producing constitutive mannanase and its application

A technology of NTT33C6 and alkalophilic bacillus, applied in the field of microorganisms, can solve the problems of high production cost, high processing cost, high consumption of raw materials and energy consumption, etc., achieve high production stability, avoid secondary pollution, and reduce production costs Effect

Active Publication Date: 2019-08-06
曹军卫 +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems of the chemical degumming method: first, the process is long, consumes a lot of raw materials and energy consumption, resulting in high production costs; second, the use of high-concentration acid, alkali, oxidant, etc., causes great damage to the fiber and poor fiber quality , so it is difficult to meet the requirements of spinning high-count yarn; the third is that the COD of degumming sewage produced Cr (chemical oxygen demand) can be as high as 15000-25000mg / mL, and BOD 5 / COD Cr <0.3, belongs to very difficult sewage, in order to meet the discharge standard, it needs to be treated after a large amount of dilution, so the treatment cost is high, and it is difficult to meet the standard, so it will seriously pollute the environment (Wu Junnan, Hao Xinmin, Tang Zongliu, etc. Hemp fiber high temperature— Flash explosion combined degumming technology [J], Textile Journal, 2007, 28(11):76-80.)
[0012] But from the current point of view, many biological degumming methods cannot be applied to industrial production, mainly because the enzyme activity is too low, and the raw hemp after enzyme degumming still contains more colloids, and its effect is only equivalent to the pretreatment process of chemical degumming The effect of (Yu Chunhua, Feng Xinxing, Jia Changlan, etc. Hemp fiber high temperature-enzyme combined degumming technology [J]. Textile Journal, 2007, 28 (6): 79-82. Cai Xia, Xiong Heping, Yan Li, etc. Cannabis microorganisms— Steam Explosion Combined Degumming Technology[J]. Textile Journal, 2011,32(7):75-79.)
Therefore, until now, there is no example of the successful use of cannabis biological degumming method in production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of alkalophilic bacillus ntt33c6 producing constitutive mannanase and its application
  • A kind of alkalophilic bacillus ntt33c6 producing constitutive mannanase and its application
  • A kind of alkalophilic bacillus ntt33c6 producing constitutive mannanase and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The isolation and screening method of alkalophilic bacillus NTT33C6, its steps are:

[0054] 1) Sample collection

[0055] Soil sampling was carried out from the Zhelimu League area of ​​Inner Mongolia Autonomous Region, China. 1g of the soil sample was suspended in sterile water and mixed evenly; the supernatant was put into the separation medium, and its composition was: glucose 10g, peptone 5g, yeast extract 5g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.2g, agar 18g, water 1000mL, with Na 2 CO 3 Adjust the pH to 10-11; incubate at 28°C for 24-48 hours.

[0056] 2) Separation and purification

[0057] Select a single colony in the isolation medium and transfer it to the locust bean gum solid medium, the composition of which is locust bean gum 10g, yeast extract 5g, peptone 5g, K 2 HPO 4 1g, MgSO 4 ·7H 2O 0.1g, agar 18g, water 1000mL, with Na 2 CO 3 Adjust the pH to 10-11; select the bacterial strains that produce a transparent circle around the colony, and place th...

Embodiment 2

[0076] The application and effect analysis of Bacillus alkalophilus NTT33C6 in the biological degumming of cannabis, the steps are:

[0077] 1) The obtained mutant strain NTT33C6 was cultured in locust bean gum liquid medium at 37°C, 200 rpm, and activated for 36-48 hours;

[0078] 2) transfer to the culture medium containing hemp respectively, shake at 37°C, 200rpm, and cultivate for 6-8 hours; 4 ) 2 SO 4 1.5g, K 2 HPO 4 0.2g, MgSO 4 ·7H 2 O0.25g, water 150mL, and use Na 2 CO 3 Adjust pH to 10-11;

[0079] 3) Observe the fiber dispersion in the culture medium, measure the residual glue rate, and analyze the lipid waxy, water-soluble, pectin, hemicellulose, lignin and cellulose content of the hemp raw hemp and the fiber after biological degumming [Chinese people Republic "Quantitative Analysis Method for Chemical Components of Ramie" (GB5889-86)]. Its measurement method and calculation formula are as follows:

[0080] ①Determination of moisture content of raw hemp ...

Embodiment 3

[0140] The application of alkalophilic bacillus NTT33C6 in cannabis production, its steps are:

[0141] 1) carry out biological degumming to cannabis according to the method described in step 1) and 2) in Example 2;

[0142] 2) The hemp after biological degumming is dispersed like cotton, after washing the hemp once, in a solution containing 0.2-0.4% mass concentration of NaOH and 0.1% mass concentration of sodium tripolyphosphate, 0.2Mpa, scouring 1-2 Hour.

[0143] 3) According to the general treatment method of chemical degumming (Jingang. Research progress of hemp degumming method [J]. Journal of Nanjing Forestry University, 2009, 33 (4): 140-144.) Washing, beating hemp, bleaching (available chlorine 0.5-0.8g / L), water washing, pickling (pH2.5), water washing, drying, oiling, drying, and drying to obtain fine-dried hemp fiber that meets national quality standards and production needs.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses an alkalophilic bacillus NTT33C6. The preservation number of the strain is CCTCC M2016316, which has the ability to produce constitutive alkaline β-mannanase; the bacterium is rod-shaped, and the size is 0.5-0.65×2.6 ~3.4μm, flagella lateral, motile, endospore oval; aerobic type, colony round, smooth surface, with concentric circles. Bacillus alkalophilus NTT33C6 can be used for biological degumming of cannabis, with a degumming rate of over 70% in 6-8 hours; the degumming rate of over 80% in 6-8 hours for hemp that has not been removed from the husk; and the degumming waste liquid is easy to handle The combined use of biological degumming and chemical degumming provided by the present invention can produce lean hemp with a certain residual glue rate requirement according to production needs.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an alkalophilic bacillus NTT33C6 producing constitutive mannanase, and at the same time relates to a method for separating and screening the alkalophilic bacillus NTT33C6, and also relates to the use of the strain in cannabis biological degumming and cannabis production use. Background technique [0002] Marijuana (Cannabis sativa L.) belongs to the family Moraceae (Maraceae) and belongs to the genus Cannabis. Annual erect herb, 1-4 meters high. Hemp has the biological characteristics of barren tolerance, strong stress resistance, wide adaptability, light-loving (especially short-day) and high photosynthetic efficiency. [0003] China has a long history of growing marijuana and using hemp products. Fragments of marijuana from the Shang Dynasty have been unearthed in Henan, my country, and hemp seeds unearthed in Linxi, Dongxiang, Gansu are also more than 5,00...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20D01C1/04C12R1/07
CPCC12N9/2491C12Y302/01025C12N1/205C12R2001/07D01C1/04
Inventor 曹军卫朱雨兰夏晨阳涂毅
Owner 曹军卫
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com