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Anti-aging probiotic preparation and preparation method thereof

A probiotic preparation and anti-aging technology, applied in biochemical equipment and methods, methods based on microorganisms, anti-toxic agents, etc., can solve the problems of probiotics with less anti-aging functions, and achieve strong tolerance, simple preparation, and broad The effect of market prospects

Active Publication Date: 2017-02-15
漯河微康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Probiotics are a type of active microorganisms that are beneficial to the host. A large number of reports have confirmed that probiotics have a variety of physiological effects. Probiotics can improve the intestinal environment, regulate the balance of flora, enhance the body’s immunity, and relieve allergic symptoms. There are relatively few researches on the anti-aging function of human body, mainly focusing on the regulation of intestinal flora, anti-oxidation, immune regulation, etc.

Method used

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  • Anti-aging probiotic preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of anti-aging probiotic powder

[0036] S1. Activate Bifidobacterium longum BL21 and Lactobacillus plantarum Lp90 in a test tube respectively to obtain a first-grade seed solution, and then transfer the first-grade seed solution to the seed medium to obtain a second-grade seed solution. 40°C, anaerobic culture for 8-24 hours; the secondary seed liquid is respectively connected to the fermentation medium for high-density fermentation, the inoculum amount of the secondary seed liquid connected to the fermentation medium is 1%-5%, and the culture condition is 30-40°C, anaerobic culture for 8-18 hours, until the cell density in the fermentation liquid reaches 10 9 , respectively collect the fermentation broth;

[0037] Wherein, the seed culture medium is MRS liquid medium, the pH value of MRS liquid medium is 6.5~6.8, and MRS liquid medium comprises: 2% lactose, 1% peptone, 0.5% yeast extract, 0.5 % sodium acetate, 0.2% diammonium hydrogen citrate, 0...

Embodiment 2

[0041] Example 2 In vitro acid resistance and bile salt resistance test of probiotics

[0042] A. Determination of acid resistance of strains

[0043] Method: The bacterial powder was inserted into the simulated artificial gastric juice (pH=2.5) for 3 hours according to the inoculum amount of 1% (w / v), and the samples were taken to count plate colonies, and the number of viable bacteria at 0 hours was used as a control to calculate the survival rate of the strains .

[0044] B. Determination of strain tolerance to bile salts

[0045] Method: The bacterial powder was inoculated into the simulated artificial intestinal fluid (0.3% bile salt concentration) according to the inoculum amount of 1% (w / v) and cultivated for 3 hours, and the plate colonies were counted by sampling. Rate.

[0046] The result is as figure 1 As shown, the survival rate of Bifidobacterium longum BL21 and Lactobacillus plantarum Lp90 after acidification and bile salt treatment were all above 60%, and th...

Embodiment 3

[0047] Example 3 Determination of Free Radical Scavenging Ability and Superoxide Dismutase (SOD) Activity

[0048] A. Materials

[0049] SOD kit, H 2 o 2 , FeSO 4 , Pyrogallol, O-phenanthroline and other reagents are analytical reagents.

[0050] B. Method

[0051] 1) Preparation of cell-free extract

[0052] Two strains (Bifidobacterium longum BL21, Lactobacillus plantarum Lp90) were continuously cultured in MRS medium at 37°C for 3 generations, collected by centrifugation at 8000r / min for 20min, washed 3 times with PBS buffer and resuspended in PBS buffer , the number of live bacteria is adjusted to 10 10 cfu / mL, break the cells in an ultrasonic cell disruptor in an ice bath, and collect the supernatant after centrifugation at 4°C for 30 min.

[0053] 2) 2 strains are sensitive to superoxide free radicals (O 2 - ) Determination of scavenging capacity

[0054] Pyrogallol autoxidation colorimetric analysis method was adopted, the Tris-HCl buffer solution with a conce...

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Abstract

The invention relates to a biological preparation, and in particular to an anti-aging probiotic preparation and a preparation method thereof. The anti-aging probiotic preparation consists of effective doses of bifidobacterium longum BL21, lactobacillus plantarum Lp90 and adjuvants. The bifidobacterium longum BL21 is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC NO.10452; and the lactobacillus plantarum Lp90 is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC NO.10453. According to the anti-aging probiotic preparation and the preparation method provided by the invention, by regulating an intestinal flora, keeping intestinal flora balance and inhibiting the growth of harmful bacteria, body aging can be delayed.

Description

technical field [0001] The invention relates to a biological preparation, in particular to an anti-aging probiotic preparation and a preparation method. Background technique [0002] The problem of aging in my country is becoming more and more serious, and the proportion of the elderly population is increasing. The search for anti-aging active substances and the exploration of their mechanisms have become the research hotspots of today's aging problems. At present, the anti-aging activity of various natural products has been confirmed, but due to their single source, difficulty in separation and purification, and high cost, it is difficult to obtain a wide range of applications. As a potential anti-aging substance, probiotics have a wide range of sources, relatively high safety, and low price, and are gradually popular among the public. [0003] Probiotics are a type of active microorganisms that are beneficial to the host. A large number of reports have confirmed that prob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23K10/18A61K35/745A61K35/747A61P39/06C12R1/01C12R1/25
CPCA61K35/745A61K35/747C12N1/20A23V2002/00A23V2400/169A23V2400/533A23V2200/302A23V2200/3204A61K2300/00
Inventor 方曙光杨帆姜甜赵阿丹占英英陈珂可
Owner 漯河微康生物科技有限公司
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