Method for rapidly separating trichoderma in plant rhizosphere saline-alkali soil

A separation method, the technology of Trichoderma, applied in the field of microorganism separation, can solve the problems of less species diversity, difficult separation methods, single species of Trichoderma, etc., to solve the difficulty of separation technology, solve the problem of low separation efficiency, and speed up the collection progress Effect

Pending Publication Date: 2017-02-15
GANSU AGRI UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, this method has not been further studied for the special conditions of saline-alkali soil, and some of its extraction conditions are not suitable for the rapid separation of Trichoderma in saline-alkali soil.
[0004] In view of the problems of low Trichoderma community density, less species diversity, difficult separation method, low separation speed and efficiency, and single species of Trichoderma obtained in the plant rhizosphere saline-alkaline soil, the present invention further develops a kind of trichoderma suitable for plant rhizosphere. Rapid separation technique of Trichoderma in saline-alkaline soil

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  • Method for rapidly separating trichoderma in plant rhizosphere saline-alkali soil

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Embodiment 1

[0019] A rapid isolation method of Trichoderma in plant rhizosphere saline-alkali soil, comprising the following steps:

[0020] A. Take soil samples: Remove the dry soil on the surface, take the 0-20cm soil layer soil, put it in room temperature to air dry, and sieve through a sieve with a mesh of 1mm; then according to the area where the soil is collected, the soil texture, and the crops Classify and number the soil samples according to environmental conditions, etc., and measure the pH of soil samples with different numbers;

[0021] B. Preparation of soil dilution: Accurately weigh 10 g of the saline-alkaline soil sample to be tested, put it into a 150 mL Erlenmeyer flask filled with 90 mL of sterile water, add 10 glass beads and shake at 150 rpm for 2 hours on a shaker at 25 ° C to make the soil Evenly disperse the microorganisms, and then let it stand for 25s, which is 10 times of soil dilution, and then dilute it to 1000 times of soil dilution for later use;

[0022] C...

Embodiment 2

[0026] A rapid isolation method of Trichoderma in plant rhizosphere saline-alkali soil, comprising the following steps:

[0027] A. Take soil samples: Remove the dry soil on the surface, take the 0-20cm soil layer soil, put it in room temperature to air dry, and sieve through a sieve with a mesh of 1mm; then according to the area where the soil is collected, the soil texture, and the crops Classify and number the soil samples according to environmental conditions, etc., and measure the pH of soil samples with different numbers;

[0028] B. Preparation of soil dilution: Accurately weigh 10 g of the saline-alkali soil sample to be tested, put it into a 150 mL Erlenmeyer flask filled with 90 mL of sterile water, add 10 glass beads and place on a shaker at 25°C at 130 rpm for 2.5 hours to make the soil The microorganisms in the solution are evenly dispersed, and then left to stand for 20s, which is a 10-fold soil dilution, and then diluted to a 1000-fold soil dilution for later us...

Embodiment 3

[0033] A rapid isolation method of Trichoderma in plant rhizosphere saline-alkali soil, comprising the following steps:

[0034] A. Take soil samples: Remove the dry soil on the surface, take the 0-20cm soil layer soil, put it in room temperature to air dry, and sieve through a sieve with a mesh of 1mm; then according to the area where the soil is collected, the soil texture, and the crops Classify and number the soil samples according to environmental conditions, etc., and measure the pH of soil samples with different numbers;

[0035] B. Preparation of soil dilution: Accurately weigh 10 g of the saline-alkaline soil sample to be tested, put it into a 150 mL Erlenmeyer flask filled with 90 mL of sterile water, add 10 glass beads and shake at 160 rpm on a shaker at 25°C for 1.5 hours to make the soil The microorganisms in the solution are evenly dispersed, and then left to stand for 20 seconds, which is a 10-fold soil dilution, and then diluted to a 100-fold soil dilution for ...

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Abstract

The invention provides a method for rapidly separating trichoderma in plant rhizosphere saline-alkali soil. The method mainly comprises the steps that a method for collecting and sampling the plant rhizosphere saline-alkali soil is adopted; soil treatment and soil diluent preparation are performed after collection; screening and improvement of a separated culture medium are performed; activation, culture, identification and other technologies of separated strains are adopted. Technologies for determining the number of separated bacterial colonies of the trichoderma in different types of plant rhizosphere saline-alkali soil and developing the trichoderma in the plant rhizosphere saline-alkali soil are adopted. By the adoption of the method, the separation efficiency, separation speed and species diversity of the trichoderma in the plant rhizosphere saline-alkali soil can be remarkably improved, and 1000 times of soil diluent can serve as the best dilution concentration for rapid separation of the trichoderma in the plant rhizosphere saline-alkali soil. In addition, the method is simple, convenient and easy to operate. The method can be used for separation of trichoderma in different types of habitat soil and different types of plant rhizosphere soil, further the problems of low separation efficiency and high separation technology difficulty of the trichoderma in the saline-alkali soil are solved, and the trichoderma resource collecting progress is accelerated.

Description

technical field [0001] The invention relates to the field of microorganism separation, in particular to a rapid separation method of Trichoderma in plant rhizosphere saline-alkali soil. Background technique [0002] Trichoderma belongs to the Fungal Kingdom, Deuteromycota, Hyphospora, Hyphospora, Polyporaceae, and Trichoderma, which widely exist in soil under different environmental conditions. Since the mid-19th century, humans have had a preliminary understanding of Trichoderma, but it was not until the 1960s that the taxonomic status of Trichoderma was determined. Most Trichoderma can produce a variety of biologically active substances that have antagonistic effects on plant pathogenic fungi, bacteria and insects, such as cell wall degrading enzymes and secondary metabolites, and can improve the stress resistance of crops, promote plant growth and improve agricultural products. Therefore, it is widely used in biological control, biological fertilizer and soil conditioner...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12R1/885
CPCC12N1/14C12N1/02
Inventor 张树武徐秉良刘佳薛应钰梁巧兰
Owner GANSU AGRI UNIV
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