Polyethyleneimine imprinted polymer probe and its preparation method and application
A polyethyleneimine, imprinted polymer technology, applied in fluorescence/phosphorescence, instruments, analytical materials, etc., can solve the problems of difficult separation and recovery, complicated operation, weak anti-interference ability, etc.
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[0045] As a preferred embodiment of the present invention, the preparation method of the polyethyleneimine imprinted polymer probe at least includes the following steps:
[0046] According to the ratio of polyethyleneimine: crosslinking agent: metal ion=(1~2) g: (1~1.5) g: (2.5~5) mmol, weigh the polyethyleneimine, crosslinking agent and Water-soluble metal salts;
[0047] Dissolving and mixing the polyethyleneimine and the water-soluble metal salt respectively, then raising the temperature to 60-100°C, and adding the cross-linking agent;
[0048] After adding the cross-linking agent, lye should be added to adjust the pH value to 9-11, and the reaction solution should be kept at 60-100°C for 2-5 hours;
[0049] Dialyze the reaction product until the conductivity of the dialysate is close to that of distilled water to obtain metal ion imprinted nanomaterials;
[0050] According to the mass ratio of the metal ion-imprinted nanomaterial:fluorescein=(4.5-5.5):1, the synthesis pr...
Embodiment 1
[0066] A preparation method of polyethylenimine imprinted polymer probe, comprising the following steps:
[0067] (1) Weigh 5gPEI, 3.025g copper nitrate trihydrate, 5gECHA, 0.1666g fluorescein respectively;
[0068] (2) Dissolve 3.025g of copper nitrate trihydrate in 30mL of water, add 5g of PEI and stir to dissolve, place the mixed solution in an ultrasonic cleaner and ultrasonically vibrate for 30min to achieve coordination equilibrium;
[0069] (3) Warm up the coordination balanced solution in (2) to 80°C, and add the prepolymer ECHA drop by drop with mechanical stirring;
[0070] (4) Adjust the pH of the reaction system to 10 with 1mol / L NaOH solution after 30 minutes of reaction after the dropwise addition, keep stirring at 80°C for 5 hours and then stop;
[0071] (5) Put the reaction product in (4) into a dialysis bag with a molecular cut-off of 100k, and repeatedly dialyze and wash with 1mol / L hydrochloric acid and ethylenediaminetetraacetic acid (EDTA) to wash away te...
Embodiment 2
[0077]The preparation method of Example 2 is the same as that of Example 1, except that the metal ion imprinting is zinc ion imprinting, and the water-soluble zinc salt used is zinc nitrate.
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