Method, kit and application for rapidly extracting genomic dna from hazelnut oil

Abstract

The invention discloses a method for quickly extracting genome DNA from forest frog's oviduct, comprising the steps: (1) taking a forest frog's oviduct sample, adding Chelex-100 and protease K to the forest frog's oviduct sample, carrying out water bath heat insulation first, then terminating at high temperature, carrying out cell cracking, and centrifuging to obtain first supernatant, wherein the Chelex-100 is used for chelating non-nucleic acid organic matters in the forest frog's oviduct sample and inducing protein denaturation to facilitate the protease K to carry out enzymolysis, and the protease K is used for digesting protein in the forest frog's oviduct sample; (2) adding phenol / chloroform / isoamyl alcohol to the first supernatant for removing residual lipoid and protein; and (3) precipitating DNA by using isopropanol or absolute ethyl alcohol. The invention also provides a kit for quickly extracting genome DNA from the forest frog's oviduct, and application of a DNA bar code technology in identification of the forest frog's oviduct. The extraction method disclosed by the invention can realize quick and effective extraction of the genome DNA from dried forest frog's oviduct medicinal materials, and provides a basis for wider application of the DNA bar code technology.

Description

technical field [0001] The invention relates to the field of genomic DNA extraction of traditional Chinese medicinal materials, in particular to a method, a kit and an application thereof for rapidly extracting the genomic DNA of toad oil. Background technique [0002] The identification of traditional Chinese medicine is the premise and basis for studying the variety and quality of traditional Chinese medicine, formulating the standard of traditional Chinese medicine, and finding and expanding the source of medicine. The four traditional identification methods of traditional Chinese medicine are base identification, character identification, microscopic identification and physical and chemical identification. The theoretical basis of traditional identification methods is based on the analysis of the traits of taxa. These traits are phenotypes closely related to the growth and development stages of species and the environment, and are easily affected by subjective and object...

AI Technical Summary

Problems solved by technology

[0004] However, the Chinese Pharmacopoeia animal medicine toad oil sample is not a fresh dry material sample, rich in mucus, colloid and hemicelluloses and other substances. The toad oil medicinal material is highly swollen after the solution, and the expansion degree is as high as more than 55 times. After centrifugation in a water bath, there is no supernatant or very little supernatant, and the subsequent DNA extraction steps of the toad oil medicinal material cannot be performed, which is not conducive to subsequent DNA barcode identification and other work.

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