SERS substrate based on metal dot matrix, preparation method of SERS substrate and method for conducting Raman detection through SERS substrate

Abstract

The invention provides an SERS substrate based on a metal dot matrix. The SERS substrate comprises a silicon substrate body, the metal dot matrix formed on the silicon substrate body and a hydrophobic layer arranged in an area outside the metal dot matrix. A metal layer of the metal dot matrix is made of gold or silver or copper. The area outside the metal dot matrix on the substrate has hydrophobicity, the positions of metal dots of the metal dot matrix are relatively hydrophilic, therefore, to-be-detected substances can be better enriched on the metal dots, and Raman signals can be enhanced. The invention further provides a preparation method of the substrate, a method for conducting Raman detection through the substrate and an SERS chip based on the metal dot matrix.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to a SERS substrate based on a metal lattice, a preparation method thereof, and a Raman detection method using the substrate. Background technique [0002] Surface-enhanced Raman scattering (SERS) is widely used in food safety testing, medical diagnosis, material characterization, archaeology, criminal investigation and other fields due to its advantages of high sensitivity, non-destructive testing and in-situ measurement. . Especially in the field of hazardous substance detection and material characterization, SERS is a very important analytical method. [0003] The advantages of SERS technology are very clear, but when the SERS technology is introduced to the laboratory level, the actual promotion of SERS technology is limited due to the lack of SERS active substrates suitable for practical applications. Today's SERS active substrates include two types, solid and liquid. Al...

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Problems solved by technology

[0003] The advantages of SERS technology are very clear, but when the SERS technology is introduced to the laboratory level, the actual promotion of SERS technology is limited due to the lack of SERS active substrates suitable for practical applications

Today's SERS active substrates include two types, solid and liquid. Although solid active substrates have controllable periodic hot spots and can ensure the reproducibility of SERS measurements, such substrates cannot be stored for a long time. preparation, which increases the difficulty of some processes; and the liquid active substrate, although it can be stored for a long time, can be used immediately in SERS analysis, but the uncontrollable aggregation of nanoparticles in the liquid substrate will lead to the recurrence of SERS analysis during SERS analysis. poor sex

Moreover, most of the current SERS technologies simply use metal nanoparticles on the substrate to construct "hot spots", which have limited gain effects on Raman signals.

[0004] The above-mentioned problems have hindered the pace of SERS technology from the research level to market application, and restricted the development of practical application of SERS technology.

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