Enhanced Raman substrate for detecting streptomycete hyphae, and preparation and use methods thereof
A Raman substrate, Streptomyces technology, applied in Raman scattering, measuring devices, instruments, etc., can solve problems such as being unsuitable for the detection of large-scale samples, complex microbial serotypes, and cumbersome detection processes, and to promote miniaturization and Integration, shortened detection time, enhanced Raman spectrum
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Embodiment 1
[0036] Example 1: Preparation of nylon microporous filter membrane multilayer silicon dioxide silver-plated Raman detection substrate
[0037] 1. First, inject 1-100mL of 100-500nm diameter silica nanoparticle solution into a detachable filter with a diameter of 1-10cm through a syringe. The diameter of the detachable filter is 1-10cm and the filter hole diameter Assemble a multi-layer ordered structure on a nylon microporous filter membrane between 100-450 nm (smaller than the particle size of silica nanoparticles) to obtain a monodisperse silica nanoparticle layer;
[0038] 2. Inject 1-50 mL of the previously prepared gold nanoparticle solution with a particle size of 1-50nm into the filter, and filter it to the surface of the silica nanoparticle to obtain a gold nanoparticle layer;
[0039] 3. Take out the nylon microporous filter membrane covered with the gold nanoparticle layer, place it on a hot table and dry it, then immerse it in electroless silver electroplating solution A (...
Embodiment 2
[0040] Example 2: Detection of the Raman spectrum of the dye 4-ATP
[0041] 1. Cut the pre-prepared nylon microporous filter membrane multilayer silica silver-plated substrate into a size of 3mm×3mm;
[0042] 2. Use a micropipette to take 5μL, 10 -3 ~10 -12 The 4-ATP dye of M was dropped onto the above-mentioned substrate, and then the Raman spectroscopy of 4-ATP was detected by the confocal Raman microscope to obtain the Raman spectrum of the dye 4-ATP;
[0043] 3. After the reaction is completed, discard the used substrate. The substrate is a disposable substrate.
Embodiment 3
[0044] Example 3: Detection of the Raman spectrum of the dye R6G
[0045] 1. Cut the pre-prepared nylon microporous filter membrane multilayer silica silver-plated substrate into a size of 3mm×3mm;
[0046] 2. Use a micropipette to take 5μL, 10 -3 ~10 -12 The R6G dye of M is dropped onto the above-mentioned substrate, and then the Raman spectroscopy of R6G is detected by the confocal Raman microscope to obtain the Raman spectrum of the dye R6G;
[0047] 3. After the reaction is completed, discard the used substrate. The substrate is a disposable substrate.
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