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Bacillus tequilensis JN369 and application thereof

A technology of Bacillus tequila and Bacillus, which is applied in the direction of application, bacteria, and fungicides, can solve the problems of reduced antimicrobial control effect, unsatisfactory effect, and poor colonization ability of antagonistic microorganisms, etc., to achieve good control effect, Both protective and therapeutic effects, the effect of avoiding adverse effects

Active Publication Date: 2017-01-04
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the field of biological control, there are often problems such as poor colonization ability of antagonistic microorganisms under natural conditions in the field and unstable effect on disease control.
A large number of studies have also shown that the antagonistic microorganisms screened indoors have a strong inhibitory effect on pathogenic bacteria in the greenhouse, but the effect in field control is not satisfactory. Colonize the root circle of plants, on the other hand, interact with the complex microbial species and antagonistic strains in the field soil, resulting in a decrease in the control effect of antagonistic microorganisms. Therefore, it is necessary to select and screen biological strains with strong field adaptability or high-efficiency metabolites Applying to field disease control is an important way to solve the above problems

Method used

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  • Bacillus tequilensis JN369 and application thereof
  • Bacillus tequilensis JN369 and application thereof
  • Bacillus tequilensis JN369 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 The separation and acquisition of Bacillus tequila JN369

[0028] Healthy rice plants were selected from the diseased clusters of rice blast-susceptible rice variety Xiangzaoxian 24 (N28°42′55.56″, E112°15′74.24″) in Huatian Village, Shiniujiang Town, Taojiang, Hunan Province, and a total of 517 strains were isolated , an antagonistic bacteria JN369 with a 79.95% inhibition rate on M.

[0029] Bacterial strain JN369 ( figure 2 A and figure 2 B), observe the colony morphology, observe the scanning electron microscope images of the bacteria, and combine the physiological and biochemical and microbiological detection results of the bacteria, and the 16S rDNA sequencing results (see SEQ ID NO.1), to finally determine the bacterial strain JN369 is B. tequilensis.

[0030] In addition, the plate confrontation culture method was used to find that the strain JN369 had inhibitory effects on a variety of pathogenic indicator bacteria ( image 3 ), such as tobacco re...

Embodiment 2

[0031] Example 2 Application of Bacillus tequila JN369 in the Control of Rice Blast

[0032] After the bacterial strain JN369 was activated on the NA solid medium plate, two bacterial cakes with a diameter of 5mm were punched out and inserted into 150mL NB liquid medium (peptone 5g / L, beef extract 3g / L, glucose 20g / L L, pH 7.0, prepared by distilled water) in a 250mL Erlenmeyer flask, 28 ° C, 180r / min conditions in a shaker for 6 days, filtered with a bacterial filter to obtain the metabolites of Bacillus tequila JN369.

[0033] Mix the metabolites of the bacterial strain JN369 and the PDA medium with a volume (mL) of 1:8, connect the rice blast fungus cake with a diameter of 7mm on the prepared medium, and replace the antagonistic bacteria culture medium with sterile water for Control, cultured at 28°C, measured the size of the fungus cake of Magnaporthe oryzae after 10 days, repeated 3 times, the results showed that the inhibition rate of Mycelium of Magnaporthe oryzae was 7...

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Abstract

The invention relates to the technical field of biological control, and in particular discloses bacillus tequilensis JN369 and an application thereof in preventing and controlling rice blast. The preservation number of the strain JN369 is CCTCC No:M2016340. With the application of the strain JN369 to prevent and control the rice blast, potential adverse impact of chemical control on environment and rice safety and disease infection risk of using disease-resistant varieties can be avoided. The living strain JN369 and metabolites thereof can take a relatively good effect on preventing the rice blast, and the strain JN369, which integrates both protecting and treating effects, can be processed and developed in the dosage forms of the living strain and the metabolites. In addition, the strain JN369 has an inhibitory effect on various pathogen indicating bacteria.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a bacillus tequila JN369 and its application in preventing and treating rice blast. Background technique [0002] Rice (Oryza sativa) is one of the most important food crops, and more than half of the world's population feeds on rice. Rice blast (the pathogen is Magnaporthe oryzae, and its asexual generation is Pyiicularia oryzae) is one of the most serious fungal diseases of rice. M. oryzae is a semi-living parasite with a distinct biotrophic phase during the initial stages of infection. Rice blast can be divided into seedling blast, leaf blast, knot blast, panicle blast, grain blast, and branch blast according to the location and time of onset. At present, the production mainly uses disease-resistant varieties and chemical fungicides to control rice blast. The main fungicides used are tricyclazole, rice blastling, prochloraz, rice blastamide, isoframine, rice blas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 刘二明邹秋霞任佐华刘敏捷周鑫钰刘双清
Owner HUNAN AGRICULTURAL UNIV
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