Application of magnolia odoratissma alkyl diol in preparation of composition having nerve restoration function
A technology of magnolanediol and neurological functions, which is applied in drug combinations, nervous system diseases, active ingredients of hydroxyl compounds, etc., and can solve the problems of inability to enhance cell repair and regeneration, low blood-brain barrier permeability, and short half-life, etc. problem, to achieve the effect of good clinical application prospect
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Embodiment 1
[0030] Example 1: A screening model for promoting nerve regeneration in vitro using the neuroma cell line N1E115 as the experimental object
[0031] The invention establishes a screening model for promoting the axon growth of N1E115 cells in vitro. Undifferentiated N1E115 cells were cultured in DMEM medium containing 10% fetal bovine serum FBS, 1% penicillin-streptomycin, placed in a constant temperature and humidity of 5% CO 2 , cultured in a 37°C incubator; the induced differentiation N1E115 was cultured in DMEM medium without FBS, 1% penicillin-streptomycin, and placed in a constant temperature and humidity of 5% CO after adding the test compound 2 , continue culturing in a 37°C incubator for 3-4 days, and then take pictures under a 20X microscope, randomly photograph 8 fields of view in each well, and count 50 cells in each field of view to count the percentage of differentiated cells and the change of cell axon length. (Yamashita H, Muroi Y, Ishii T. Saccharin enhances n...
Embodiment 2
[0043] Example 2: A screening model for promoting nerve regeneration in vitro using the undifferentiated rat adrenal pheochromoma cell line PC-12 as the experimental object
[0044]In the present invention, the undifferentiated rat adrenal pheochromoma cell line PC-12 is selected, and under the condition induced by low concentration of NGF, a screening model for promoting nerve regeneration is established to evaluate the action effect of magnolanediol. (Ishima T, NishimuraT M, Hashimoto K. Potentiation of nerve growth factor-induced neurite outgrowthin PC12 cells by donepezil: role of sigma-1 receptors and IP3 receptors. [J]. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2008, 32 (32): 1656-9.)
[0045] Undifferentiated PC-12 cells were cultured in DMEM medium containing 10% horse serum HS, 5% fetal bovine serum FBS, 1% penicillin-streptomycin, placed in constant temperature and humidity 5% CO 2 , cultured in an incubator at 37°C; the PC-12 cells induced to ...
Embodiment 3
[0053] Example 3: A screening model for promoting nerve regeneration in vitro with primary cortical neuron cells as the experimental object
[0054] Primary cortical neuronal cells were selected to demonstrate the neuroregenerative effects of magnolanediol. Take suckling rats from pregnant rats at 16-18 days of pregnancy, separate cerebral cortex cells, inoculate in 24-well plates pre-coated with PLL, add neuron culture medium, add different concentrations after 4 hours of cell attachment The magnolanediol (0.1, 1, 10 μM) was cultured for 24 hours and photographed, and 8 fields of view were randomly photographed in each well under a 20X microscope, and 50 cells in each field of view were counted. Measure and analyze the nerve cell protrusions in the selected microscope pictures.
[0055] The results show that magnolanediol can significantly promote the length of axons, the number of axons, and the branching of dendrites after acting on primary cortical neurons of rats for 24 ...
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