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Method for deeply fermenting soybean meal by virtue of compound phytase and glucose oxidase

A technology of glucose oxidase and deep fermentation, which is applied in the direction of food preservation, food ingredients as antimicrobial preservation, animal feed, etc., and can solve the problem of susceptibility to mold pollution, affecting piglets' feed palatability and utilization of phytate phosphorus Low efficiency and other problems, to achieve the effect of improving production performance, increasing the utilization rate of phosphorus resources, and reducing waste

Inactive Publication Date: 2017-01-04
江西英泰瑞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current fermented soybean meal uses lactic acid bacteria, Bacillus subtilis and yeast for collaborative fermentation, but they failed to degrade and eliminate phytate phosphorus and mycotoxins during the fermentation process, resulting in low utilization of phytate phosphorus. Moreover, the feed raw materials are susceptible to mold contamination during production and transportation, which affects the palatability and feed intake of piglets.

Method used

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  • Method for deeply fermenting soybean meal by virtue of compound phytase and glucose oxidase
  • Method for deeply fermenting soybean meal by virtue of compound phytase and glucose oxidase
  • Method for deeply fermenting soybean meal by virtue of compound phytase and glucose oxidase

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Add 203kg of phytase with a specification of 250U-350U / kg to the mixed bacterial solution; 122kg of glucose oxidase with a specification of 150U-250U / kg, spray it into 1000kg of soybean meal through a double-axis stainless steel mixer, and control the moisture at 830kg After stirring evenly, carry out box-type solid fermentation. The fermentation temperature is controlled at 30 degrees, and the fermentation is terminated after 72 hours of continuous fermentation. The fermentation material is dried using a fluidized bed drying equipment. After drying, the moisture content is controlled within 210, and then crushed. Weigh and pack for storage.

Embodiment 2

[0027] Add 190kg of phytase with a specification of 250U-350U / kg to the mixed bacterial solution; 114kg of glucose oxidase with a specification of 150U-250U / kg, spray it into 1000kg of soybean meal through a double-axis stainless steel mixer, and control the water content at 850kg After stirring evenly, carry out box-type solid fermentation. The fermentation temperature is controlled at 35 degrees. After 72 hours of continuous fermentation, the fermentation is terminated. The fermentation material is dried using fluidized bed drying equipment. After drying, the moisture content is controlled within 190, and then crushed. Weigh and pack for storage.

Embodiment 3

[0029] Add 176kg of phytase with a specification of 250U-350U / kg to the mixed bacterial solution; 105kg of glucose oxidase with a specification of 150U-250U / kg, spray it into 1000kg of soybean meal through a double-axis stainless steel mixer, and control the water content at 870kg After stirring evenly, carry out box-type solid fermentation. The fermentation temperature is controlled at 40 degrees. After 72 hours of continuous fermentation, the fermentation is terminated. The fermentation material is dried using fluidized bed drying equipment. After drying, the moisture content is controlled within 170, and then crushed. Weigh and pack for storage.

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Abstract

The invention discloses a method for deeply fermenting soybean meal by virtue of compound phytase and glucose oxidase. The method comprises the following steps: (1) respectively inoculating lactic acid bacteria, bacillus subtilis and saccharomycetes into different liquid fermentation tanks, and fermenting for 18 hours; (2) adding phytase and glucose oxidase into prepared bacteria solutions; (3) spraying the bacteria solutions into the soybean meal, controlling moisture, uniformly stirring, and continuously fermenting for 72 hours; (4) drying, grinding, weighing, packaging and storing fermented materials. Preferably, the configuration proportion of lactic acid bacteria to bacillus subtilis to saccharomycetes is 100 to 1 to 10; and the specifications of phytase and glucose oxidase are respectively 250U / kg-350U / kg and 150U / kg-250U / kg, and the proportion is 1 to (0.6-0.8). The method has the beneficial effects that the utilization ratio of phytate phosphorus in the soybean meal is increased, the phosphorus resource is saved, the environmental pollution is reduced, the mycotoxins such as aspergillus flavus are degraded, escherichia coli and salmonella in animal intestines are inhibited, and the production properties of beasts and birds are improved.

Description

technical field [0001] The invention relates to a method for deeply fermenting soybean meal with compound phytase and glucose oxidase, which belongs to the technical field of biological mold decomposition and fermentation. Specifically, it improves the utilization rate of phosphorus element through phytase and strengthens the anti-toxic bacteria through glucose oxidase. suppression. Background technique [0002] my country is one of the largest breeding countries in the world. With the development of animal husbandry, the breeding industry is gradually becoming industrialized and large-scale, accompanied by the vigorous development of the feed industry, and the production and sales of feed are increasing year by year. One of the most important vegetable protein sources in the world, its crude protein content is as high as 43% to 48%. As a high-quality bio-fermented protein raw material, fermented soybean meal utilizes plant-based protein to solve the problems of digestion an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/37A23K10/12A23K10/14A23L3/3571
CPCA23L3/3571A23V2002/00A23V2200/10Y02P60/87
Inventor 周景汉
Owner 江西英泰瑞生物科技有限公司
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