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Horse sperm low-temperature and freeze preservation diluent

A cryopreservation and cryopreservation technology, applied in the biological field, can solve problems such as undiscovered

Active Publication Date: 2017-01-04
新疆畜牧科学院畜牧研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Relevant searches did not find reports similar to the claims of this patent

Method used

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  • Horse sperm low-temperature and freeze preservation diluent
  • Horse sperm low-temperature and freeze preservation diluent
  • Horse sperm low-temperature and freeze preservation diluent

Examples

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Embodiment 1

[0032] The preparation of embodiment 1 mixed antibiotics

[0033] Weigh 2 g of gentamycin, 6 g of neomycin, 6.8 g of apramycin, 2.4 g of colistin, and 0.01 g of amphotericin, dissolve them in 40 ml of deionized water, and place them in a refrigerator at 4°C for later use.

Embodiment 2

[0034] The preparation of embodiment 2 combined antifreeze

[0035] Combination 1: 1 / 3 Glycerin and 2 / 3 Methylformamide

[0036] Combination 2: 2 / 3 Glycerin and 1 / 3 Methylformamide

[0037] Combination 3: 1 / 3 Glycerin and 2 / 3 DMF

[0038] Combination 4: 2 / 3 Glycerin and 1 / 3 DMF

[0039] Combination 5: 1 / 3 methylformamide and 2 / 3 dimethylformamide

[0040] Combination 6: 1 / 3 dimethylformamide and 2 / 3 methylformamide

[0041] Combination 7: 1 / 3 Glycerin 1 / 3 Methylformamide and 2 / 3 DMF

Embodiment 3

[0042] Embodiment 3 sugar salt solution

[0043] Weigh 0.33g of sodium bicarbonate, 4.766g of hydroxyethylpiperazineethanesulfonic acid, 0.375g of sodium citrate dihydrate, 0.615g of potassium citrate monohydrate, 0.2g of sodium hydroxide, 27g of lactose, 18.27g of glucose, inositol Dissolve 0.01g in 800ml of deionized water, add 1ml of mixed antibiotics, stir evenly, then add deionized water to a 1000ml volumetric flask to make up to 1000ml, and place in a 4°C refrigerator for later use.

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Abstract

The invention belongs to the field of biotechnology and relates to preparation of animal sperm low-temperature and freeze preservation diluents, in particular to a horse sperm low-temperature and freeze preservation liquid. The sperm low-temperature and freeze preservation diluent is prepared by combining sodium bicarbonate, hydroxyethylpiperazine ethane sulfonic acid, trisodium citrate dihydrate, potassium citrate monohydrate, sodium hydroxide, lactose, glucose, inositol, gentamicin, neomycin, apramycin, colistin, amphotericin, sodium pyrophosphate, sodium caseinate, whey protein, an antioxidant and deionized water. The horse sperm low-temperature and freeze preservation diluent can effectively increase the survival rate of sperms obtained after unfreezing of low-temperature and freezing of horse seminal fluid and the hybridization conception rate. Compared with a conventional method, the method is easy and stable to implement and especially remarkable in technical effect.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the production of animal semen low temperature and cryopreservation liquid, in particular to a low temperature and cryopreservation diluent for horse sperm. Background technique [0002] my country is a big country in the horse industry, and the number of horses ranks second in the world. As a major large animal, horses used to be closely related to human production and life. After the rise of the machinery industry, the service value of horses weakened and gradually faded out of people's lives. In recent years, with the improvement of people's living standards in our country, the modern horse industry, which mainly focuses on products and cultural leisure, has quietly emerged in my country. The development of the modern horse industry urgently requires the use of artificial insemination to improve horses. Low temperature and cryopreservation) are the core technologies in the process of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 毋状元郑新宝罗永明于维浩董红霍飞徐文慧阿依诺尔·亚森陈静波李海吕雪峰
Owner 新疆畜牧科学院畜牧研究所
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