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Somatic embryo of vitis amurensis, as well as culture method, culture medium and applications of somatic embryo

A technology of somatic embryos and culture methods, which is applied in the field of grape tissue culture, can solve the problems of inability to guarantee the continuity of extraction work, low resveratrol content, and restrictions on the scale of industrial production of resveratrol

Active Publication Date: 2016-12-21
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology for extracting resveratrol mainly uses grape plants or grape fruits as raw materials for extraction, but the raw materials of this extraction method are limited by seasons and growth cycles, and the continuity of the extraction work cannot be guaranteed
Therefore, developed the use of grape callus as a raw material to extract the resveratrol contained therein, although the limitations of the growing season and cycle have been overcome, the existing culture methods cultivated and used as raw materials for extracting resveratrol The content of resveratrol in the callus of Vitis vinifera is low, which limits the industrial production scale of extracting resveratrol from the callus culture of Vitis vinifera

Method used

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  • Somatic embryo of vitis amurensis, as well as culture method, culture medium and applications of somatic embryo
  • Somatic embryo of vitis amurensis, as well as culture method, culture medium and applications of somatic embryo

Examples

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Embodiment 1

[0040] In this embodiment, the tender stem section of a variety of Vitis vinifera Zuoshan is used as the exosome of Vitis vitis vinifera, and the tender stem section of Vitis vitis vinifera is obtained from the National Fruit Tree Germplasm Zuojiashan Grape Resource Garden of the Institute of Special Products of the Chinese Academy of Agricultural Sciences. In other embodiments, the variety of Vitis vinifera is not limited to Zuoshanyi, and may be Beibinghong, Zuoyouhong, Shuanghong, Shuangfeng or Shuangyou and other varieties.

[0041] The culturing method of the mountain grape somatic embryo of the present embodiment comprises the steps:

[0042] (1) Rinse the taken tender stems of mountain grapes with tap water for 4 hours, then disinfect them with 70% alcohol for 30 seconds on an ultra-clean workbench, pour out the alcohol, disinfect them with 0.1% mercury liter for 5 minutes, and rinse them with sterile water for 3 minutes. ~4 times.

[0043] (2) Put the young grape stem...

Embodiment 2

[0051] The culturing method of the mountain grape somatic embryo of the present embodiment comprises the steps:

[0052] (1) Rinse the taken tender stems of mountain grapes with tap water for 4 hours, then disinfect them with 70% alcohol for 30 seconds on an ultra-clean workbench, pour out the alcohol, disinfect them with 0.1% mercury liter for 5 minutes, and rinse them with sterile water for 3 minutes. ~4 times.

[0053] (2) Put the tender stem section of Vitis vinifera into a petri dish, cut it into about 0.4 cm long stem section of Vitis vitis vinifera under aseptic conditions, that is, exosomes.

[0054] (3) Inoculate the cut vine stem sections into the Erlenmeyer flasks containing the pre-medium for pre-cultivation, inoculate 3 exosomes in each Erlenmeyer flask, and cultivate in dark under the pre-cultivation conditions at a temperature of 27°C 28 days to induce callus formation.

[0055] Wherein, the pre-medium used was prepared as follows: MS medium was used as the ba...

Embodiment 3

[0061] The culturing method of the mountain grape somatic embryo of the present embodiment comprises the steps:

[0062] (1) Rinse the taken tender stems of mountain grapes with tap water for 4 hours, then disinfect them with 70% alcohol for 30 seconds on an ultra-clean workbench, pour out the alcohol, disinfect them with 0.1% mercury liter for 5 minutes, and rinse them with sterile water for 3 minutes. ~4 times.

[0063] (2) Put the tender stem section of Vitis vinifera into a petri dish, and cut it into about 0.5 cm long section of Vitis vitis vinifera under aseptic conditions, that is, exosomes.

[0064] (3) Inoculate the cut vine stem sections into the Erlenmeyer flasks containing the pre-medium for pre-cultivation, inoculate 3 exosomes in each Erlenmeyer flask, and cultivate in dark under the pre-cultivation conditions at a temperature of 27°C 28 days to induce callus formation.

[0065] Wherein, the pre-medium used was prepared as follows: MS medium was used as the bas...

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Abstract

The invention discloses a somatic embryo of vitis amurensis, as well as a culture method, a culture medium and applications of the somatic embryo, belonging to the technical field of tissue culture of the grape. According to the somatic embryo, the culture method, the culture medium and the applications, by selecting and controlling the carbon source, the ventilatory capacity and the nitrogen source of the inducing culture medium of the somatic embryo, in the culture process, the growth of the somatic embryo and the synthesis of resveratrol achieve the optimal state, and the cultured somatic embryo has high content of resveratrol. With the adoption of the culture method of the somatic embryo of vitis amurensis, and the somatic embryo obtained through the method, a material and a basis are provided for the industrial preparation and industrial development of the secondary metabolite, namely, resveratrol through the culture on the vitis amurensis cells.

Description

technical field [0001] The invention relates to the technical field of grape tissue culture, in particular to a grapevine somatic embryo and its culture method, culture medium and application. Background technique [0002] Resveratrol, a secondary metabolite of Vitis amurensis Rupr., has many beneficial effects on the human body, such as preventing cancer, protecting the cardiovascular system, antibacterial, and regulating blood lipids. The existing technology for extracting resveratrol mainly extracts grape plants or grape fruits as raw materials, but the raw materials of this extraction method are limited by seasons and growth cycles, and the continuity of the extraction work cannot be guaranteed. Therefore, developed the use of grape callus as a raw material to extract the resveratrol contained therein, although the limitations of the growing season and cycle have been overcome, the existing culture methods cultivated and used as raw materials for extracting resveratrol ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C07C37/68C07C39/21
CPCC07C37/68C12N5/04C07C39/21
Inventor 孙丹艾军秦洪艳许培磊赵滢刘迎雪王振兴杨义明范书田
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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