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Needle milling production process of powdery cell culture medium

A production process and culture medium technology, which is applied in the field of needle grinding production technology of powder cell culture medium, can solve the problems of reducing the annual production capacity of the culture medium, and achieve the effects of saving production time, production cost and large output.

Inactive Publication Date: 2016-12-14
TIANXINHE SUZHOU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If you continue to use the ball milling method, the annual production capacity of the medium will be greatly reduced

Method used

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  • Needle milling production process of powdery cell culture medium
  • Needle milling production process of powdery cell culture medium
  • Needle milling production process of powdery cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In Example 1, the pin mill production process was adopted, and the preparation of DMEM / F12 medium was taken as an example. The specific method steps are as follows.

[0028] Step 1. Weigh the raw materials by type and set aside; the raw materials are weighed by type as shown in Table 1-4:

[0029] Table 1: Inorganic salts in the formula

[0030] components

Content (mg / L)

calcium chloride

116.6

copper sulfate

0.0013

Ferric nitrate nonahydrate

0.05

Ferrous Sulfate Heptahydrate

0.417

potassium chloride

311.8

Magnesium chloride hexahydrate

28.64

Anhydrous Magnesium Sulfate

48.84

Sodium chloride

6999.5

Anhydrous Sodium Dihydrogen Phosphate

54.35

Anhydrous Disodium Hydrogen Phosphate

71.02

Zinc sulfate heptahydrate

0.432

[0031] Table 2: Amino acids in the formula

[0032]

[0033]

[0034] Table 3: Vitamins in Formula

[0035] com...

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PUM

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Abstract

The invention discloses a needle milling production process of a powdery cell culture medium. The process includes the steps of: 1) weighing raw materials according to types for later use; 2) pre-mixing the weighed raw materials in the step 1); 3) grinding and pulverizing the pre-mixed raw materials by means of a needle-type grinding miller; 4) final-mixing the pulverized materials in the step 3); and 5) packaging the final-mixed product. The process can achieve continuous production of the cell culture medium, wherein the productivity of the process is equal to that of a process with a large-size ball miller. The process is less in occupied area of equipment, saves production cost and production time, is high in productivity, is less in batch difference and has stable product quality.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a needle-milling production process of a powdered cell culture medium. Background technique [0002] Synthetic cell culture medium is an artificially prepared mixed substance suitable for the nutritional requirements of cell growth. It is generally a medium prepared from natural ingredients such as body fluids and serum digestive juices, as well as a synthetic medium or a comprehensive medium prepared from chemical raw materials. Medium. From the perspective of the physical properties of the medium, it can be divided into solid medium, liquid medium and powder medium with coagulant added. Most domestic enterprises adopt the traditional ball milling method. Although the structure of the ball mill is simple, the cleaning and handling of the steel balls is time-consuming and laborious, the efficiency is low, the working noise is loud, and the floor space is large, which increases the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00
CPCC12N5/0037C12N2500/12C12N2500/30C12N2500/32C12N2500/34C12N2500/38
Inventor 赵洪磊
Owner TIANXINHE SUZHOU BIOTECH CO LTD
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