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High-flux chip data processing and analysis process control method for circular RNA

A process control and data processing technology, applied in the fields of design medical genomics and computational biology, can solve the problems of not knowing how to analyze and inaccurate, and achieve the effect of simple implementation method, reduced scientific research cost, and wide application range.

Inactive Publication Date: 2016-12-07
SOUTHEAST UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a high-throughput chip data processing and analysis process control method for circular RNA, to solve the inaccuracy of the existing technology in the high-throughput chip data processing of circRNA, and not knowing how to analyze circRNA And other issues

Method used

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  • High-flux chip data processing and analysis process control method for circular RNA
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  • High-flux chip data processing and analysis process control method for circular RNA

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Embodiment

[0089] Firstly, the raw data is filtered, and then the data with low-quality signal and noise pollution are removed, and the effective circular RNA expression value is obtained after normalization. Based on the analysis results of circular RNAs, the function prediction of them can be combined with the co-expressed gene expression profiles; the regulatory analysis of competitive endogenous RNAs can also be carried out based on their sequence characteristics. Schematic diagram of circular RNA-coexpression gene network image 3 shown. On the basis of the above analysis, a series of statistical and visual analysis can be carried out.

[0090] 1. The circular RNA original signal file is shown in Table 1

[0091] Analysis platform: R platform

[0092] Analysis software: Affy, limma

[0093] Table 1

[0094]

[0095] Explanation of column names:

[0096]

[0097] 2. The expression results of the circular RNA chip are shown in Table 2

[0098] Analysis platform: R platfor...

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Abstract

The invention discloses a high-flux chip data processing and analysis process control method for circular RNA. The method comprises the steps of generating a self-defined parameter configuration file by a system first and then generating a batch processing executable file corresponding to a data process according to a self-defined parameter file after parameter setting by a user and a high-flux chip data processing process module; and executing the batch processing executable file by the system, implementing data process automation, and finally generating a result report file. Therefore, bio-information analyzers can be efficiently assisted to finish a set of standardized high-flux data analysis process, and even scientific research personnel who do not understand high-flux data analysis can be enabled to finish the high-flux data analysis. In addition, the purposes of optimizing working efficiency of the scientific research personnel and reducing scientific research cost can be achieved. Reliable and diversified circular RNA analysis methods are proposed; and the control method also can be used for high-flux data analysis processes of long-chain non-coding RNA and the like, and is universal in different fields, simple to implement and wide in application range.

Description

technical field [0001] The invention is designed in the fields of medical genomics and computational biology, and specifically relates to a high-throughput chip data processing and analysis process control method for circular RNA. Background technique [0002] Circular RNA (circular RNA, circRNA) is a recently discovered special type of non-coding RNA, which exists in large quantities in the cytoplasm of eukaryotic cells, and is mainly produced by pre-mRNA through alternative splicing. With a closed circular structure, most circular RNAs are composed of exon sequences, which are conserved in different species and have expression specificity in tissues and different developmental stages. Because circular RNA is insensitive to nucleases, it is more stable than linear RNA, which makes circular RNA have obvious advantages in the development and application of new clinical diagnostic markers. However, there are many problems in using circular RNA as a marker. For example, a larg...

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Application Information

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IPC IPC(8): G06F19/12G06F19/28
CPCG16B50/00G16B5/00
Inventor 陈瑞高娜李晓波孟庆涛吴申申
Owner SOUTHEAST UNIV
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