Symbiotic culture device, culture method and application of bifidobacteria and bacillus natto

A technology of Bacillus natto and symbiotic culture, applied in the directions of bifidobacteria, sterilization methods, microorganism-based methods, etc., can solve the problems of high requirements on growth and nutrient conditions, difficulty in preparation and long-term preservation, and high difficulty in culture and proliferation, Achieve reasonable structure design and process arrangement, improve broiler growth performance and immune function, and avoid external microbial infection.

Active Publication Date: 2017-08-04
宁波大世生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, researchers at home and abroad have carried out a lot of research work on Bifidobacterium and Bacillus natto microecological feed additives, but most of the research has focused on the development and application of single strain culture or compound live bacterial preparations. , and Bifidobacterium requires high nutritional conditions for its growth, has no spores, and is a strict anaerobic bacteria. Therefore, it is difficult to cultivate and proliferate under normal conditions, and its resistance to external adverse factors is weak, so it is difficult to prepare and maintain for a long time. save

Method used

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  • Symbiotic culture device, culture method and application of bifidobacteria and bacillus natto
  • Symbiotic culture device, culture method and application of bifidobacteria and bacillus natto

Examples

Experimental program
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Effect test

Embodiment 1

[0041] 1. Symbiosis culture device for bifidobacteria and Bacillus natto

[0042] See attached picture:

[0043] A symbiotic culture device for bifidobacteria and Bacillus natto includes a fermentor 2 installed on a base 1. A rotating device 3 is installed in the fermentor 2 and a culture container 4 for holding symbiotic culture medium is placed on the rotating device 3 A titration device 5 for containing bifidobacteria that extends into the top of the culture container is installed on the lid of the fermentation tank 2 through the tank cover, and the titration device 5 may be provided with a flow regulating valve;

[0044] A temperature sensor 6 and an oxygen sensor 7 are also provided in the fermentation tank 2; a hot air supply device 8 is installed on one side of the fermentation tank, and the hot air supply device 8 is installed on the upper end surface of the base. The upper part is connected, the conveying pipe 9 is provided with a filtering and sterilizing device 10, and th...

Embodiment 2

[0062] 1. Symbiosis culture device for bifidobacteria and Bacillus natto

[0063] Same as Example 1

[0064] 2. Symbiosis culture method of Bifidobacterium and Bacillus natto

[0065] Specific steps are as follows:

[0066] (1) Preparation of symbiosis medium: the symbiosis medium is made of the following raw materials by weight: 22 parts of bean dregs, 11 parts of rice bran, 4 parts of NaCl, K 2 HPO 4 2 parts, NH 4 H 2 PO 4 3 parts, (NH 4 ) 2 SO 4 4 parts, MgSO 4 ·7H 2 O 4 parts, KNO 3 5 servings, vitamin B 1 13 parts, 10 parts of silkworm pupa powder, 225 parts of corn steep liquor, 14 parts of Tween-80, 9 parts of inulin oligosaccharides, 2 parts of Sophora flavescens powder, 2 parts of Radix Isatidis powder;

[0067] The preparation method is as follows: a. Soak the bean dregs in 5 times distilled water for 9 hours, then grind and homogenize, the homogenization pressure is 140MPa, centrifugal separation, and take the supernatant;

[0068] b. Mix the unused raw materials, add 4 ti...

Embodiment 3

[0076] 1. Symbiosis culture device for bifidobacteria and Bacillus natto

[0077] Same as Example 1

[0078] 2. Symbiosis culture method of Bifidobacterium and Bacillus natto

[0079] Specific steps are as follows:

[0080] (1) Preparation of symbiosis medium: The symbiosis medium is made of the following raw materials by weight: 25 parts of bean dregs, 12 parts of rice bran, 6 parts of NaCl, K 2 HPO 4 3 parts, NH 4 H 2 PO 4 5 parts, (NH 4 ) 2 SO 4 5 parts, MgSO 4 ·7H 2 O 5 parts, KNO 3 6 servings, vitamin B 1 15 parts, 15 parts of silkworm pupa powder, 250 parts of corn steep liquor, 15 parts of Tween-80, 10 parts of inulin oligosaccharides, 3 parts of Sophora flavescens powder, 3 parts of Radix Isatidis powder;

[0081] The preparation method is as follows: a. Soak the bean dregs in 5 times distilled water for 10 hours, then grind and homogenize, the homogenization pressure is 140MPa, centrifugal separation, and take the supernatant;

[0082] b. Mix the unused raw materials, add 4 ...

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Abstract

The invention discloses a bifidobacterium and bacillus natto symbiotic culture device, culture method and application, comprising a fermenter, a rotating device is arranged in the fermenter, and a container for containing a culture medium of bacillus natto is placed on the rotating device The culture container, the tank cover of the fermenter is installed with a titration device that extends above the culture container and is used to hold the bifidobacteria. The symbiotic cultivation of bifidobacterium and bacillus natto can be realized by the reasonable cooperation of the sterilization device, the circulation suction device, the deoxygenation device and the control device. The invention is applied to broiler chicken feed, and significantly improves the growth performance and immune function of broiler chickens. The device and method for cultivating symbiotic bifidobacteria and bacillus natto of the present invention are novel and reasonable, have remarkable effects in application to animal feed, and have broad application prospects.

Description

[0001] Technical field: [0002] The invention relates to the technical field of bacterial symbiosis culture, in particular to a symbiosis culture device, culture method and application of bifidobacteria and Bacillus natto. [0003] Background technique: [0004] The colony of Bifidobacterium is smooth, convex, intact, creamy to white, shiny, and has a soft texture. Bifidobacterium is a normal physiological bacteria in humans and animals. It colonizes the intestine and is the dominant flora of the intestine. Its number is closely related to the health of the body and is currently recognized as a type of bacteria that promotes the health of the body. The representative beneficial bacteria. The bacterium can form a physiological barrier on the surface of the intestinal mucosa to resist the invasion of pathogenic bacteria such as Salmonella typhi, diarrhea-causing Escherichia coli, Girella dysentery, and maintain the normal microecological balance in the intestinal tract of the body; i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/12C12M1/04C12N1/20A23K50/75A23K10/18C12R1/01C12R1/07
CPCC12M29/22C12M29/24C12M37/02C12M37/04C12M41/00C12M41/12C12M41/34C12N1/20A23V2400/51
Inventor 胥振国蔡玉华范高福方丽波刘修树袁星向敏黄鹤春宫斌
Owner 宁波大世生命科技有限公司
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