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Bifidobacterium and bacillus natto symbiotic culture device and method and application of method

A technology of Bacillus natto and bifidobacteria, which is applied in the directions of bifidobacteria, sterilization methods, microorganism-based methods, etc., can solve the problems of difficult preparation and long-term storage, high requirements for growth and nutrient conditions, and high difficulty in culture and proliferation. , to achieve the effect of improving growth performance and immune function, reasonable structure design and process arrangement, and avoiding external microbial infection

Active Publication Date: 2016-12-07
宁波大世生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, researchers at home and abroad have carried out a lot of research work on Bifidobacterium and Bacillus natto microecological feed additives, but most of the research has focused on the development and application of single strain culture or compound live bacterial preparations. , and Bifidobacterium requires high nutritional conditions for its growth, has no spores, and is a strict anaerobic bacteria. Therefore, it is difficult to cultivate and proliferate under normal conditions, and its resistance to external adverse factors is weak, so it is difficult to prepare and maintain for a long time. save

Method used

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  • Bifidobacterium and bacillus natto symbiotic culture device and method and application of method
  • Bifidobacterium and bacillus natto symbiotic culture device and method and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Bifidobacterium and Bacillus natto symbiotic culture device

[0037] See attached picture:

[0038] Bifidobacterium and Bacillus natto symbiotic culture device, including a fermenter 2 installed on a base 1, a rotating device 3 is arranged in the fermenter 2, and a culture container 4 for holding a symbiotic medium is placed on the rotating device 3 , the tank cover of the fermenter 2 is installed with a titration device 5 that extends into the top of the culture container and is used to hold the bifidobacteria. The titration device 5 can have a flow regulating valve;

[0039]Temperature sensor 6, oxygen sensor 7 are also arranged in fermentation tank 2; One side of fermentation tank fermenter is provided with hot blast supply device 8, and hot blast supply device 8 is installed on the upper end surface of base, and hot blast supply device 8 is connected with the fermenter by conveying pipeline 9. The upper part is connected, the conveying pipeline 9 is provided wit...

Embodiment 2

[0057] 1. Bifidobacterium and Bacillus natto symbiotic culture device

[0058] Exactly the same as Example 1

[0059] Two, bifidobacterium and bacillus natto symbiotic culture method

[0060] Specific steps are as follows:

[0061] (1) Preparation of symbiotic medium: symbiotic medium is made of the following raw materials in parts by weight: 22 parts of bean dregs, 11 parts of rice bran, 4 parts of NaCl, K 2 HPO 4 2 parts, NH 4 h 2 PO 4 3 parts, (NH 4 ) 2 SO 4 4 parts, MgSO 4 ·7H 2 O 4 parts, KNO 3 5 parts, vitamin B 1 13 parts, 10 parts of silkworm chrysalis powder, 225 parts of corn steep liquor, 14 parts of Tween-80, 9 parts of inulin oligosaccharide, 2 parts of Sophora flavescens powder, 2 parts of Radix Radix Radix;

[0062] The preparation method is as follows: a. Soak the bean dregs with 5 times of distilled water for 9 hours, grind and homogenize, the homogenization pressure is 140MPa, centrifuge, and take the supernatant;

[0063] b. Mix the unused ...

Embodiment 3

[0071] 1. Bifidobacterium and Bacillus natto symbiotic culture device

[0072] Exactly the same as Example 1

[0073] Two, bifidobacterium and bacillus natto symbiotic culture method

[0074] Specific steps are as follows:

[0075] (1) Prepare the symbiotic medium: the symbiotic medium is made of the following raw materials in parts by weight: 25 parts of bean dregs, 12 parts of rice bran, 6 parts of NaCl, K 2 HPO 4 3 parts, NH 4 h 2 PO 4 5 parts, (NH 4 ) 2 SO 4 5 parts, MgSO 4 ·7H 2 O 5 parts, KNO 3 6 parts, vitamin B 1 15 parts, 15 parts of silkworm chrysalis powder, 250 parts of corn steep liquor, 15 parts of Tween-80, 10 parts of inulin oligosaccharides, 3 parts of Sophora flavescens powder, 3 parts of Radix Radix Radix;

[0076] The preparation method is as follows: a. Soak the bean dregs with 5 times of distilled water for 10 hours, grind and homogenize, the homogenization pressure is 140MPa, centrifuge, and take the supernatant;

[0077] b. Mix the unu...

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Abstract

The invention discloses a bifidobacterium and bacillus natto symbiotic culture device and method and application of the method. The device comprises a fermentation tank. A rotating device is arranged in the fermentation tank. A culture container for containing a bacillus natto culture medium is placed on the rotating device. A titration apparatus stretching to the place above the culture container and used for containing bifidobacterium is installed on a tank cover of the fermentation tank and used in reasonable cooperation with a temperature sensor, an oxygen sensor in the tank body, a hot air supply, a filtration sterilization device, a cycle extraction device, an oxygen removal device and a control device to achieve symbiotic culture of bifidobacterium and bacillus natto, wherein the temperature sensor and the oxygen sensor are in the tank body, and the hot air supply device, the filtration sterilization device, the cycle extraction device, the oxygen removal device and the control device are arranged outside the tank body. The bifidobacterium and bacillus natto symbiotic culture device and method are applied to broiler feed, and remarkably improve broiler growth performance and immunity functions. The bifidobacterium and bacillus natto symbiotic culture device and method are novel, reasonable, remarkable in effect when applied to animal feed and wide in application prospect.

Description

Technical field: [0001] The invention relates to the technical field of bacterial symbiotic culture, in particular to a symbiotic culture device, culture method and application of bifidobacteria and bacillus natto. Background technique: [0002] Colonies of bifidobacteria were smooth, convex, well-edged, creamy to white, shiny and had a soft texture. Bifidobacteria are normal physiological bacteria in humans and animals. They colonize the intestinal tract and are the dominant flora in the intestinal tract. The number of them is closely related to the health of the body. representative beneficial bacteria. The bacterium can form a physiological barrier on the surface of the intestinal mucosa, thereby resisting the invasion of pathogenic bacteria such as Salmonella typhi, diarrhea-causing E. activity, enhance the immunity of body cells; can synthesize B vitamins, niacin and folic acid and other vitamins; can control endotoxemia and prevent constipation, prevent anemia and ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/12C12M1/04C12N1/20A23K50/75A23K10/18C12R1/01C12R1/07
CPCC12M29/22C12M29/24C12M37/02C12M37/04C12M41/00C12M41/12C12M41/34C12N1/20A23V2400/51
Inventor 胥振国蔡玉华范高福方丽波刘修树袁星向敏黄鹤春宫斌
Owner 宁波大世生命科技有限公司
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