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Mesenchymal stem cell injection as well as preparation method and application thereof

A technology of stem cells and injections, applied in the field of stem cells, can solve problems such as pain of patients, and achieve the effect of being convenient to use, convenient to obtain materials, and treating Parkinson's disease.

Inactive Publication Date: 2016-12-07
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research mainly uses bone marrow mesenchymal stem cells for treatment, but bone marrow collection will bring physical pain to patients

Method used

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  • Mesenchymal stem cell injection as well as preparation method and application thereof
  • Mesenchymal stem cell injection as well as preparation method and application thereof
  • Mesenchymal stem cell injection as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1 Preparation of placental mesenchymal stem cells

[0071] 1. Primary isolation of placental mesenchymal stem cells

[0072] Under sterile conditions, take the fresh placenta discarded after delivery, peel off the placenta, wash it with PBS buffer solution, and cut the placenta tissue into 5×5cm 2 , add 3-5 times the volume of 0.25% trypsin to digest, place on a 200r / min 37°C constant temperature shaker, digest for 30min, and shake vigorously several times every 10min to remove epithelial cells.

[0073] After the digestion is complete, transfer it to a storage bottle, add 150 mL of PBS buffer solution, shake vigorously, and repeat this operation 2 times. Transfer placental tissue to a small beaker and chop to 1mm 3 . Then transfer to a storage bottle, add 20 mL of 0.5% type I collagenase and complete medium (high glucose DMEM+10% FBS), so that the final concentration of collagenase is 0.1-0.2%. Place in a constant temperature shaker at 37°C, 250r / min, un...

Embodiment 2

[0087] Preparation of embodiment 2 placental mesenchymal stem cell preparation

[0088] The method for preparing 100mL stem cell injection: 30mL of human serum albumin stock solution with an albumin concentration of 10% (the final concentration of the mass volume ratio is 3%), 0.5mL of low molecular weight heparin calcium (the final concentration of the mass volume ratio is 0.5%), Vitamin C 0.5g (mass volume ratio final concentration is 0.5%), lentinan 0.4g (mass volume ratio final concentration is 0.4%) and 0.9% sodium chloride injection 61.6mL are mixed, slowly add DMSO 7mL (volume percentage Content is 7%), put into 4 ℃ of refrigerators and pre-cool, placental mesenchymal stem cells (cell final concentration 1×10 6 cells / mL) were resuspended in the mixed solution, and the prepared placental mesenchymal stem cell injection was dispensed in The cells are stored in the cryopreservation bag, and the preparation is complete.

Embodiment 3

[0089] Preparation of embodiment 3 placental mesenchymal stem cell preparation

[0090] The method for preparing 100mL stem cell injection: 5mL of human serum albumin stock solution with an albumin concentration of 20% (the final mass-volume concentration is 1%), 0.5mL low-molecular-weight heparin calcium (0.5% mass-volume final concentration), Vitamin C 0.3g (mass volume ratio final concentration is 0.3%), lentinan 0.2g (mass volume ratio final concentration is 0.2%) and 0.9% sodium chloride injection 88.8mL are mixed, slowly add DMSO 5mL (volume percentage Content is 5%), put into 4 ℃ of refrigerators and pre-cool, placental mesenchymal stem cells (cell final concentration 1×10 6 cells / mL) were resuspended in the mixed solution, and the prepared placental mesenchymal stem cell injection was dispensed in The cells are stored in the cryopreservation bag, and the preparation is complete.

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Abstract

The invention relates to the technical field of stem cells, in particular to a mesenchymal stem cell injection as well as a preparation method and application thereof. The mesenchymal stem cell injection comprises mesenchymal stem cells, human serum albumins, low-molecular weight heparin calcium, vitamin C, lentinan, dimethyl sulfoxide and a menstruum. The mesenchymal stem cell injection provided by the invention can effectively maintain the activity of the stem cells, can increase the content of striatal dopamine and can effectively treat the Parkinson's disease.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a mesenchymal stem cell injection and its preparation method and application. Background technique [0002] Parkinson's disease (PD), also known as Parkinson's disease, is a common neurodegenerative disease characterized by progressive loss of striatal dopaminergic neuron function. In 1817, British doctor James Parkinson first described the disease in detail. Its clinical manifestations mainly include resting tremor, slow movement, muscle rigidity and posture and gait disturbance. At the same time, patients may be accompanied by non-motor symptoms such as depression, constipation and sleep disturbance. . The prevalence of PD in people over 65 years old in my country is about 1.7%. Most Parkinson's disease patients are sporadic cases, and less than 10% of patients have a family history. The exact etiology of Parkinson's disease is still unclear. Genetic factors, environmenta...

Claims

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Application Information

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IPC IPC(8): A61K38/38A61K35/28A61K31/375A61K31/715A61K47/36A61K47/20A61K9/08A61P25/16
CPCA61K9/0019A61K9/08A61K31/375A61K31/715A61K35/28A61K38/38A61K47/20A61K47/36A61K2300/00
Inventor 陈海佳葛啸虎王一飞李丽娟王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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