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Method for extracting ice structuring proteins (ISPs) from winter wheat in cold region

A technology for ice-structured protein and winter wheat, which is applied in the preparation methods of peptides, chemical instruments and methods, plant peptides, etc., can solve the problems of high cost, limited raw materials for ice-structured protein extraction, and complicated extraction process, and achieves diverse and good shapes. Ice crystal modification properties, the effect of small ice crystal size

Inactive Publication Date: 2016-11-09
HARBIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems in the prior art that the extraction raw materials for ice structural protein are limited, the extraction process is complicated, the cost is high, and the extracted ice structural protein cannot be widely used in frozen food and medicine, a kind of ice structural protein extracted from cold winter wheat is proposed. Method for Extracting Ice Structural Proteins

Method used

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Experimental program
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specific Embodiment approach 1

[0024] Specific embodiment one: the present embodiment extracts the method for ice structure protein from winter wheat in cold region and carries out according to the following steps:

[0025] 1. Preparation of ice puck

[0026] Place glass balls in each ice hole of the silicone ice tray, pour distilled water into the ice holes, and then freeze in a -18°C refrigerator to obtain ice balls;

[0027] 2. Extraction of winter wheat ISPs in cold regions

[0028] According to the ratio of solid to liquid: 1g: (10-30mL), mix cold winter wheat flour with phosphate buffer solution, stir and extract at room temperature for 0.5-3.0h, and then centrifuge for 14-16min in a centrifuge to obtain a supernatant;

[0029] 3. Ice puck extraction

[0030] Add the ice ball prepared in step 1 to the supernatant of step 2, put it in a refrigerator at -18°C, and extract it on ice for 2 to 4 minutes. At this time, the ice structure protein adsorbed by the ice ball reaches saturation. extract, and ob...

specific Embodiment approach 2

[0039] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: the diameter of the glass ball described in step 1 is 1 cm; the diameter of the obtained ice ball is 2 cm; the temperature resistance of the silica gel ice tray is -40°C Silicone ice tray, the silicone ice tray is plate-shaped, with a length of 24cm and a width of 7.3cm. There are 10×2 spherical ice holes on the plate, and the inner diameter of each ice hole is 2cm. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0040] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the pH of the phosphate buffer solution in Step 2 is 7.2-8.0, and the phosphate concentration is 60-140 mmol / L. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to a method for extracting ice structuring proteins (ISPs) from winter wheat in a cold region, and aims to solve the problems that in the prior art, raw materials for extracting the ISPs are limited, the extraction process is complex, the extraction cost is relatively high, and the extracted ISPs cannot be widely used in frozen foods and medicines. The method comprises the following steps: I, preparing ice balls, wherein the diameter of the ice balls is 2 cm; II, mixing flour of the winter wheat in the cold region with a phosphate buffer solution, and carrying out stirring, extraction and centrifugation; III, adding the ice balls for ice extraction, filtering out the ice balls, and allowing the ice balls to melt naturally; IV, re-extracting ISPs of the winter wheat in the cold region. As the ISPs are extracted from the winter wheat in the cold region, the safety is guaranteed; the extraction rate is 1.63% to 4.56%; the antifreeze activity is relatively high. The method is suitable for extracting the ISPs from the winter wheat in the cold region.

Description

technical field [0001] The invention relates to a method for extracting ice structural protein from cold winter wheat. Background technique [0002] Ice structuring proteins (ISPs), also known as antifreeze proteins and antifreeze proteins (AFPs), are a class of proteins produced by certain fish, insects, plants, fungi and bacteria to resist external environmental stress. The polypeptide produced by the reaction has the ability to prevent the formation of ice crystals, modify the shape of ice crystals and inhibit recrystallization. It has a certain thermal hysteresis activity, that is, it can lower the freezing point of the solution in a non-colligative form, but has little effect on the melting point. This creates a difference between the freezing point and melting point of the solution. The unique properties of ice structural proteins ensure the survival of organisms in low temperature conditions. In 2006, the Ministry of Health of my country announced that ice structura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/14
CPCC07K14/415
Inventor 陈凤莲曲敏鲍欢朱姝
Owner HARBIN UNIV OF COMMERCE
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