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Preparation technique of three-layer artficial blood vessel

A technology of artificial blood vessels and technical solutions, applied in medical science, prosthesis, etc., can solve the problems of aneurysm formation, easy deformation, dead fold, etc., reduce the formation of acute thrombus, enhance axial mechanics, and enhance radial mechanics Effect

Active Publication Date: 2016-11-09
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Researchers have used various techniques such as electrospinning, particle leaching, and phase separation to prepare artificial blood vessels with pores. However, in the process of using these techniques to prepare artificial blood vessels, they rarely consider and control the physical topological structure of the inner surface of artificial blood vessels.
A large number of basic studies have shown that the topological structure (such as smoothness and roughness) of the surface of artificial blood vessel materials will affect the adhesion of plasma proteins and platelets, as well as the adhesion and proliferation of endothelial cells and smooth muscle cells, thereby affecting the patency of artificial blood vessels
Another problem with degradable polymer artificial blood vessels is that they are easy to deform and form aneurysms after long-term implantation. One of the main reasons is that the pore structure of the artificial blood vessel material is irregular, and the degradation of the material will lead to the extracellular formation of new blood vessels. The matrix cannot form a pseudo-natural orientation arrangement, so that it cannot withstand the blood pressure in the body for a long time
In addition, the artificial blood vessels prepared in a large number of studies will form dead folds after being bent at a certain angle, which will hinder blood circulation after implantation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment 1: the preparation of polycaprolactone (PCL) three-layer artificial blood vessel

[0010] Preparation of the inner layer of the artificial blood vessel: Weigh 2.0 g of PCL with a number average molecular weight of 80,000 and add it to 10 ml of chloroform, stir and dissolve at room temperature overnight to prepare a PCL solution with a concentration fraction of 20% (mass / volume). The inner layer of the artificial blood vessel was prepared by direct printing in a fume hood at room temperature. Install the 2.0mm diameter stainless steel receiving rod on the printer. Aspirate the PCL solution into the syringe, mount the syringe on the syringe pump, and position the syringe needle 2 mm above the metal receiving rod. Set the advance speed of the syringe pump to 2ml / h, the rotational speed of the receiving rod to 100rpm, the lateral movement speed to 0.2mm / sec, and the printing time to 2min. After the preparation, the receiving rod with the inner layer of the artif...

Embodiment 2

[0013] Embodiment 2: Preparation of poly L-lactide-caprolactone (PLCL) three-layer artificial blood vessel

[0014] Preparation of artificial blood vessel inner layer: Weigh 1.5g PLCL and add it into 10ml tetrahydrofuran solvent, stir and dissolve overnight at room temperature to prepare a PLCL solution with a concentration fraction of 15% (mass / volume). The inner layer of the artificial blood vessel was prepared by direct printing in a fume hood at room temperature. Install the 3.0mm diameter stainless steel receiving rod on the printer. Inhale the PLCL solution into the syringe and install it on the syringe pump. Place the needle of the syringe at a distance of 3.0mm above the metal receiving rod, set the advancing speed of the syringe pump to 3ml / h, the rotating speed of the receiving rod to 200rpm, and the lateral movement speed to 0.4mm / sec, the printing time is 1min, after the preparation is completed, the receiving rod with the inner layer of the artificial blood vess...

Embodiment 3

[0017] Embodiment 3: polylactide (PLA) three-layer artificial blood vessel preparation

[0018]Preparation of artificial blood vessel inner layer: Weigh 1.2g PLA and add it into 10ml dichloromethane, stir and dissolve overnight at room temperature to prepare a PLA solution with a concentration fraction of 12% (mass / volume). The inner layer of the artificial blood vessel was prepared by direct printing in a fume hood at room temperature. Install the 4.0mm diameter stainless steel receiving rod on the printer. Inhale the PLA solution into the syringe and install it on the syringe pump. Place the needle of the syringe at a distance of 3.0mm above the metal receiving rod, set the advancing speed of the syringe pump to 3ml / h, the rotating speed of the receiving rod to 200rpm, and the lateral movement speed to 0.4mm / sec, the printing time is 1min, after the preparation is completed, the receiving rod with the inner layer of the artificial blood vessel is vacuum-dried.

[0019] Fa...

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Abstract

The invention provides a preparation technique of a three-layer (inner, middle and outer layers) artificial blood vessel. The inner layer is composed of a smooth compact thin layer prepared by an ink printing method and can inhibit plasma protein and platelet adhesion, prevent acute thrombosis formation and provide axial mechanical support. The middle layer is formed by winding oriented micro fiber prepared by wet spinning or melt spinning, and mainly functions in guiding tissue cells to grow into gaps of the oriented fiber to realize oriented deposition arrangement of extracellular matrix, and the oriented micro fiber can provide radial mechanical support. The outer layer is formed by winding thick polymer fiber and closely bonded with the middle layer and mainly functions in preventing folding when the artificial blood vessel is bent. The artificial blood vessel prepared by the method can remarkably increase patency rate, can utilize microenvironment at an implanting position to realize quasi-natural reshaping and regeneration and has good application prospect in the aspects of coronary artery bypass surgery, hemodialysis and cerebral and peripheral blood vessel replacement.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a method for preparing a three-layer artificial blood vessel. Background technique [0002] Vascular disease is the disease with the highest fatality rate in the world. The occurrence of this disease is often due to the narrowing or blockage of blood vessels, which leads to reduced blood flow and lack of nutrients, which damages tissues or organs. It usually manifests as coronary heart disease, cerebrovascular disease, peripheral artery disease, etc. disease and deep vein thrombosis. According to the prediction of the World Health Organization, by 2030, the number of people dying of cardiovascular-related diseases in the world will increase to 23.3 million each year. Vascular transplantation is still a routine method for the treatment of such diseases. The first choice for this type of surgery is to harvest and use the patient's own blood vessels such as the great s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/58A61L27/40A61L27/18A61L27/22A61L27/24A61L27/20
CPCA61L27/18A61L27/20A61L27/222A61L27/225A61L27/24A61L27/507A61L27/58C08L67/04
Inventor 朱美峰孔德领王恺董显豪
Owner NANKAI UNIV
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