A three-layer artificial blood vessel preparation technology

Abstract

The invention provides a method for preparing a three-layer (inner layer, middle layer and outer layer) artificial blood vessel. The inner layer is composed of a smooth and dense thin layer prepared by ink printing, which can inhibit the adhesion of plasma proteins and platelets and prevent acute thrombosis Formed while providing axial mechanical support. The middle layer is composed of oriented micron fiber windings prepared by wet spinning or melt spinning. Its main function is to guide tissue cells to grow into the oriented fiber voids to achieve oriented deposition and arrangement of extracellular matrix. At the same time, oriented micron fibers can provide radial Mechanical support. The outer layer is wound by thick polymer fibers and tightly bonded with the middle layer, mainly to prevent buckling when the artificial blood vessel is bent. The artificial blood vessel prepared by the invention can significantly improve the patency rate, and can realize quasi-natural remodeling and regeneration by using the microenvironment of the implanted site, and has good application prospects in coronary artery bypass grafting, hemodialysis, and brain and peripheral blood vessel replacement.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a method for preparing a three-layer artificial blood vessel. Background technique [0002] Vascular disease is the disease with the highest fatality rate in the world. The occurrence of this disease is often due to the narrowing or blockage of blood vessels, which leads to reduced blood flow and lack of nutrients, which damages tissues or organs. It usually manifests as coronary heart disease, cerebrovascular disease, peripheral artery disease, etc. disease and deep vein thrombosis. According to the prediction of the World Health Organization, by 2030, the number of people dying of cardiovascular-related diseases in the world will increase to 23.3 million each year. Vascular transplantation is still a routine method for the treatment of such diseases. The first choice for this type of surgery is to harvest and use the patient's own blood vessels such as the great s...

AI Technical Summary

Problems solved by technology

Researchers have used various techniques such as electrospinning, particle leaching, and phase separation to prepare artificial blood vessels with pores. However, in the process of using these techniques to prepare artificial blood vessels, they rarely consider and control the physical topological structure of the inner surface of artificial blood vessels.

A large number of basic studies have shown that the topological structure (such as smoothness and roughness) of the surface of artificial blood vessel materials will affect the adhesion of plasma proteins and platelets, as well as the adhesion and proliferation of endothelial cells and smooth muscle cells, thereby affecting the patency of artificial blood vessels

Another problem with degradable polymer artificial blood vessels is that they are easy to deform and form aneurysms after long-term implantation. One of the main reasons is that the pore structure of the artificial blood vessel material is irregular, and the degradation of the material will lead to the extracellular formation of new blood vessels. The matrix cannot form a pseudo-natural orientation arrangement, so that it cannot withstand the blood pressure in the body for a long time

In addition, the artificial blood vessels prepared in a large number of studies will form dead folds after being bent at a certain angle, which will hinder blood circulation after implantation