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Method for detecting equilibrium state of cholesterol

A cholesterol and total cholesterol technology, applied in the field of measuring biomedical data, can solve the problems of low plasma content, difficult separation and detection, etc.

Active Publication Date: 2016-10-26
广东国盛医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the above non-cholesterol steroids are similar in structure and chemical properties to cholesterol, and the content in plasma is low, it is difficult to separate and detect by general methods

Method used

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  • Method for detecting equilibrium state of cholesterol
  • Method for detecting equilibrium state of cholesterol
  • Method for detecting equilibrium state of cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1 A method for detecting cholesterol balance

[0081] 1. Solution preparation

[0082] 1) Preparation of reference solution

[0083] Accurately weigh the reference substances of 7-enecholesterol, streptosterol, campesterol, and β-sitosterol respectively, and prepare 11.00, 6.70, 18.00, and 33.00mg·L with n-hexane as the solvent. -1 solution for use as a stock solution. Take the stock solution and dilute it with n-hexane to prepare a mixed control solution with the highest mass concentration of No. 7, each containing 4 substances to be tested, and then successively dilute it step by step. The specific mass concentration is shown in the table below.

[0084] The concentration of 7-encholesterol, strepsterol, campesterol, β-sitosterol in each group of reference substances after different dilution factors in table 1

[0085]

[0086]

[0087] 2) Preparation of internal standard solution

[0088] Accurately weigh 0.0125g of 5α-cholestane in a 25mL measu...

Embodiment 2

[0116] Embodiment 2 A method for detecting cholesterol balance

[0117] 1. Solution preparation

[0118] Described with embodiment 1.

[0119] 2. Qualitative and quantitative analysis of sterols in samples

[0120] 1) Pretreatment of plasma samples

[0121] 100 μL of healthy human plasma (group A-plasma) and 100 μL of No. 4 reference solution in Table 1 (group B-mixed standard solution) are directly subjected to the following operations; group C is spiked plasma, that is, 100 μL of healthy human plasma Add 100 μL of the No. 4 reference substance solution in Table 1 (group C-spiked plasma) before the treatment, and then perform the following operations.

[0122] Put the above three groups of solutions into 10mL centrifuge tubes, add 10μL 5α-cholestane internal standard solution, and then add 1mL 1mol L -1 Potassium hydroxide ethanol solution, vortex to mix, seal with a stopper, place in a water bath at 60°C for saponification for 1 hour; after saponification, the solution i...

Embodiment 3

[0130] Embodiment 3 A method for detecting cholesterol balance (clinical samples)

[0131] According to the method described in Example 1, 71 parts of clinical plasma samples are detected, and 4 kinds of non-cholesterol steroids are detected in the plasma samples: the concentration of 7-encholesterol, streptosterol, campesterol, β-sitosterol ( μM) (as shown in Table 5), calculate the 7-ene cholesterol ratio, chain sterol ratio, campesterol ratio, β-sitosterol ratio (such as Shown in table 5), finally according to the formula in embodiment 1 "three" The cholesterol balance score of each clinical plasma sample was calculated, and the cholesterol balance status of each clinical plasma sample was obtained according to the balance score (as shown in Table 5).

[0132] Table 5 The detection result of the present invention's method to clinical sample cholesterol balance state

[0133]

[0134]

[0135]

[0136] According to the evaluation of the cholesterol balance stat...

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Abstract

The invention discloses a method for detecting the equilibrium state of cholesterol. According to the method, the equilibrium score of a sample cholesterol is calculated according to the formula that the equilibrium score of cholesterol is equal to the quotient obtained by dividing the sum of the ratio of lathosterol and the ratio of desmosterol by the sum of the ratio of campesterol and the ratio of beta-sitosterol; when the equilibrium score of cholesterol is larger than 0.9, excessive cholesterol is synthesized; when the equilibrium score of cholesterol ranges from 0.6 to 0.9, the cholesterol metabolism condition is normal; when the equilibrium score of cholesterol is smaller than 0.6 and larger than or equal to 0.3, excessive cholesterol is absorbed. By means of the method, the content of four non-cholesterol sterols in human plasma is measured, the sample can be better separated within shorter time, and the method has good precision degree, accuracy and solution stability, is suitable for researching and detecting absorption and synthesis changes of cholesterol in test researches on a large scale and provides evidence for clinical diagnosis of individual treatment. The detection method has high sensitivity and high specificity and has become one of the best methods for quantifying multiple sterol substances simultaneously at present.

Description

technical field [0001] The invention relates to a method for measuring biomedical data, in particular to a method for detecting cholesterol balance. Background technique [0002] The existence and transport form of cholesterol in plasma is plasma lipoprotein, that is, non-polar lipids in plasma, including cholesterol, are combined with hydrophilic apolipoprotein, so that lipoprotein has strong water solubility and can be transported in blood. It is known that the total cholesterol level in blood, plasma or serum is one type of biomedical data indicative of the risk of coronary arteriosclerosis. Epidemiological and clinical studies have shown that the level of low-density lipoprotein cholesterol is positively correlated with the incidence of coronary heart disease, and high-density lipoprotein cholesterol is negatively correlated with the incidence of coronary heart disease. hardening activity. Therefore, high-density lipoprotein cholesterol HDL-C and low-density lipoprotei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92G01N30/02
CPCG01N30/02G01N33/92G01N2030/025G01N2800/52
Inventor 潘树球邓杏飞
Owner 广东国盛医学科技有限公司
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