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Prealbumin detection kit with high stability

A technology for detecting kits and prealbumin, applied in biological testing, material inspection products, etc., can solve the problems of reduced use value and poor stability, and achieve the effects of high accuracy, good stability, and long storage time

Inactive Publication Date: 2016-10-26
上海睿康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The stability of prealbumin detection kits currently on the market is poor, which reduces the value of clinical use

Method used

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  • Prealbumin detection kit with high stability
  • Prealbumin detection kit with high stability
  • Prealbumin detection kit with high stability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The composition of the assay kit is as follows:

[0034] 1) The composition of reagent R1 is:

[0035]

[0036]

[0037] 2) The composition of reagent R2 is:

[0038]

[0039] The various components can be added sequentially at room temperature, or added at the same time, or separately packaged and prepared immediately before testing. The reagent detection ratio is 3:1.

[0040] The PA detection kit described in this embodiment is suitable for various types of automatic biochemical analyzers. Taking Hitachi 7080 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: two-point endpoint method, namely reagent R1 and R2 dosage are 225ul and 75ul respectively, sample volume is 6ul; 225ul reagent R1 add 6ul sample and incubate at 37℃ for 3~5min, read absorbance A0, add 75ulR2, incubate at 37℃ 5min, read the absorbance A1, calculate ΔA=A1-A0; the main detection wavelength is 340nm.

[0041] Using this reagent and the above determination met...

Embodiment 2

[0045] The composition of the assay kit is as follows:

[0046] 1) The composition of reagent R1 is:

[0047]

[0048] 2) The composition of reagent R2 is:

[0049]

[0050] The various components can be added sequentially at room temperature, or added at the same time, or separately packaged and prepared immediately before testing. The reagent detection ratio is 3:1.

[0051] The PA detection kit described in this embodiment is suitable for various types of automatic biochemical analyzers. Taking Hitachi 7080 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: two-point endpoint method, namely reagent R1 and R2 dosage are 225ul and 75ul respectively, sample volume is 6ul; 225ul reagent R1 add 6ul sample and incubate at 37℃ for 3~5min, read absorbance A0, add 75ulR2, incubate at 37℃ 5min, read the absorbance A1, calculate ΔA=A1-A0; the main detection wavelength is 340nm.

Embodiment 3

[0053] The composition of the assay kit is as follows:

[0054] 1) The composition of reagent R1 is:

[0055]

[0056] 2) The composition of reagent R2 is:

[0057]

[0058]

[0059] The various components can be added sequentially at room temperature, or added at the same time, or separately packaged and prepared immediately before testing. The reagent detection ratio is 3:1.

[0060] The PA detection kit described in this embodiment is suitable for various types of automatic biochemical analyzers. Taking Hitachi 7080 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: two-point endpoint method, namely reagent R1 and R2 dosage are 225ul and 75ul respectively, sample volume is 6ul; 225ul reagent R1 add 6ul sample and incubate at 37℃ for 3~5min, read absorbance A0, add 75ulR2, incubate at 37℃ 5min, read the absorbance A1, calculate ΔA=A1-A0; the main detection wavelength is 340nm.

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Abstract

The invention provides a prealbumin detection kit with high stability. The prealbumin detection kit comprises a R1 reagent and a R2 reagent, wherein the R2 reagent comprises the following components: a buffer, sodium chloride, a preservative, a PA antibody and a stabilizer. Compared with the prior art, the invention has the following beneficial effects: 1, A special stabilizer, choline chloride, is added into the R2 reagent. The stabilizer can change configuration of the PA antibody so as to achieve the effect of stabilizing the reagent; and though having changed the configuration of the antibody, the stabilizer will not affect titer of the antibody; and 2, tests show that the detection kit has high accuracy and good precision if applied for detection and has excellent stability and long preservation time. The kit has a wide range of application, is convenient to promote and use, and can be applied to different levels of hospitals, health prevention departments and biomedical research and development institutions to measure content of prealbumin in human serum or blood plasma samples.

Description

Technical field [0001] The invention relates to a prealbumin detection kit with high stability, belonging to the technical field of clinical in vitro detection reagents. Background technique [0002] Prealbumin, also known as transthyretin, or PA for short, is synthesized by hepatocytes and is often displayed in front of albumin during electrophoresis separation. Its half-life is about 12 hours. Clinically measuring the concentration of prealbumin in plasma is more sensitive to understanding protein malnutrition and liver dysfunction than albumin and transferrin. To understand what prealbumin means, you must know prealbumin The main reason for the high is caused by blood concentration. Another possibility is that the liver's strong metabolic capacity is also the cause of the high prealbumin. To understand what prealbumin means, it is necessary to know that liver cancer, cirrhosis, chronic active hepatitis, obstructive jaundice, etc. in liver diseases can all be manifested as lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 李伟奇林爱兰房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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