Inactivation process for viruses in egg yolk antibody against duck Tembusu virus

A technology of duck tembusu virus and egg yolk antibody, which is applied in the directions of antiviral immunoglobulin, inactivation/attenuation, microorganism-based methods, etc., can solve problems such as bacterial contamination, and achieve the effect of safe and simple method.

Inactive Publication Date: 2017-07-21
GUANGZHOU GREAT BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In addition, some bacterial contamination may also be brought during the preparation process

Method used

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  • Inactivation process for viruses in egg yolk antibody against duck Tembusu virus
  • Inactivation process for viruses in egg yolk antibody against duck Tembusu virus
  • Inactivation process for viruses in egg yolk antibody against duck Tembusu virus

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preparation example Construction

[0035] 1) Preparation of defatted egg yolk:

[0036] The highly immune eggs of ducks immunized with Tembusu inactivated vaccine were collected, and after inspection and disinfection, egg whites and yolks were separated manually or mechanically.

[0037] Wash the eggs with clean water to remove dirt, then soak them in a solution containing 0.5% bromogeramine for 20 minutes, and then dry them. Wipe the eggshell with 75% alcohol cotton, open the eggshell aseptically, separate the egg white and egg yolk, put the egg yolk in a sterilized beaker, add acidified water (adjusted with 1mol / L hydrochloric acid) according to the volume of egg yolk and acidified water 1:6 pH value to 4.5) and lower the temperature to 2-8°C, stir evenly, and stand at 2-8°C for 5 hours. After acidification is completed, add an appropriate amount of octanoic acid solution. The final concentration of octanoic acid added is the volume percentage m, where 0

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Abstract

The invention discloses an inactivation process for viruses in an egg yolk antibody against a duck Tembusu virus, belonging to the field of antibody therapeutic agents in biopharmacy. According to the invention, beta-propiolactone is used as an inactivator and <60>Co gamma rays are cooperatively utilized for inactivation of viruses in degreased egg yolk, wherein the minimal usage amount of beta-propiolactone is 0.02%, the minimal amount of beta-propiolactone and 3.0 kGy of the <60>Co gamma rays are cooperatively utilized for a sufficient inactivation reaction, and the obtained antibody is still safe and reliable after blind passage for three generations. The process provided by the invention is safe; beta-propiolactone is extremely prone to hydrolysis, and a fat metabolite beta-hydroxypropionic acid is formed after hydrolysis of beta-propiolactone, so no harm is posed to people; no residual <60>Co gamma rays is produced, and the antibody is stable and aseptic after irradiation by the <60>Co gamma rays; and the inactivation process is simple and easy to operate and free of complex procedures.

Description

technical field [0001] The invention belongs to the field of antibody therapeutic agents in biopharmaceuticals, and in particular relates to a virus inactivation process in egg yolk antibody of duck Tembusu virus. Background technique [0002] Chicken egg yolk antibody (EggYolkIm-munoglobulin) is an immunoglobulin present in the egg yolk, which is transferred from the immunoglobulin in the hen serum. Yolk antibody can be applied to the treatment of clinical diseases, such as chicken infectious bursal disease, Newcastle disease, duck plague, gosling plague, swine fever and so on. In clinical practice, the prevention and treatment effect of yolk antibody is very ideal. At the same time, the manufacturing process of yolk antibody is simple and cheap. A few years ago, it was used in a large amount in the poultry industry and played a very important role. [0003] However, there are obvious problems in antibody-based therapeutics that have not been inactivated by pathogenic micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/08A61L2/18C07K16/10C12N7/06C12N7/04C12N13/00C12R1/93A61L101/34
Inventor 魏平华王小庆
Owner GUANGZHOU GREAT BIOLOGICAL TECH
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