SPE-HPLC method for determining content of phellodendrine in Leshu lotion

Abstract

The invention relates to a SPE-HPLC method for determining a content of phellodendrine in Leshu washing lotion. The method comprises the following steps of: A, preparing aliquid of a reference substance; B, preparing aliquid of a test sample: preparing the liquidof the test sample by adopting a solid phase extraction method; C, performing the liquid chromatographic detection; D, establishing a standard curve; and E, determining a sample. The testedliquid is prepared by selecting the solid phase extraction method, the extraction recovery rate about 100 percent can be obtained, inorganic salts and organic impurities in a test sample can be effectively removed, and the service life of a chromatographic column can be prolonged. The solid phase extraction method can process the sample in batches and is easy in automation, so that the working efficiency can be improved. A majority of methods reported by the existing literatures adopt a C18 chromatographic column, and the present method selects an amino column as an analysis column, so thatcompared with the C18 chromatographic column, the amino column has the advantages of high column efficiency, sharp peak pattern and little interference. The method is simple in operation, accurate in result, good in repeatability and less in impurity interference in the analysis process.

Description

technical field [0001] The invention relates to a method for determining the content of phellodine in Leshu lotion, in particular to an SPE-HPLC method for determining the content of phellodine in Leshu lotion. Background technique [0002] Leshu lotion is a traditional Chinese medicine preparation made by experts from Liuzhou Maternal and Child Health Hospital in Guangxi according to the theory of traditional Chinese medicine and after years of clinical practice (approval number: Guiyao Zhizi Z02060005). It is composed of nine medicines, such as white fresh skin, cnidium, etc. More than 10 years of clinical application have shown that Leshu lotion has a good curative effect on gynecological vulvitis, vaginitis and other reproductive tract infections, but the preparation has been lacking in effective quality control method. [0003] In recent years, analysts have conducted extensive research on the quality control methods of traditional Chinese medicine, and established a v...

AI Technical Summary

Problems solved by technology

[0008] Insufficient: Anshu liquid and Leshu lotion in our hospital have different formulations and production processes, and the active ingredients or impurities in the preparations are also different. Therefore, the content determination method reported in the literature cannot be directly used to determine the Phellodendron cortex of Leshu lotion. alkali

[0015] Insufficient: The author previously established a method for determining the content of Phellodendron in Leshu Lotion, which can be used to determine Phellodendrine in Leshu Lotion

However, the preparation method of the test solution is directly measured after filtration. This method is simple to operate, but it cannot effectively remove fat-soluble impurities. It can be seen from the chromatogram that there are many impurities, which easily interfere with the determination. After analyzing a small amount of samples, the column efficiency is easily reduced.

[0016] In summary, due to the complex composition of traditional Chinese medicine preparations, different formulations of similar preparations, or different production processes, the active ingredients or impurities of each traditional Chinese medicine gynecological lotion are different, so the identification and content determination methods reported in the literature cannot be directly used for Leshu The quality control method of the lotion, or the method has certain defects, and needs to be further improved

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