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Pediococcus pentosaceus and application thereof

A technology of Pediococcus pentosaceus and microbial strains, applied in the application, bacteria, microorganism and other directions, can solve the problems of reducing the quality of animal products, imbalance of normal flora of animals, threats to human health, etc., to reduce the number of Escherichia coli and increase the number of lactic acid bacteria. The effect of high quantity and safety

Inactive Publication Date: 2016-10-26
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the excessive use of antibiotics are becoming more and more obvious. For example, the flora in the intestinal tract of animals is in a state of dynamic balance. After long-term consumption of antibiotics, the normal flora of animals will be out of balance, and after long-term use, it is easy to develop resistance to the body and drugs. Defects such as residues will reduce the body's immunity, thereby reducing the quality of animal products. If people eat animals fed with antibiotics, human health will also be threatened

Method used

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  • Pediococcus pentosaceus and application thereof
  • Pediococcus pentosaceus and application thereof
  • Pediococcus pentosaceus and application thereof

Examples

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Effect test

Embodiment 1

[0026] Isolation of Pediococcus pentosaceus R09:

[0027] The Pediococcus pentosaceae R09 of the present invention is isolated from the intestinal tract of healthy pigs, and the isolation adopts the plate dilution method, and the medium for isolation is 2% CaCO 3 SL medium, M17 medium and MRS agar medium.

[0028] Among them, each liter of SL medium includes: casein hydrolyzate 10g, yeast extract 5g, diammonium citrate 2g, sodium acetate 25g, magnesium sulfate 0.58g, agar 15g, glucose 20g, Tween 801.0ml, dipotassium hydrogen phosphate 6g, ferrous sulfate 0.03g, manganese sulfate 0.15g, distilled water 1000ml.

[0029] Each liter of M17 medium includes: phytopeptone 5.0g, yeast extract 5.0g, polypeptone 5.0g, ascorbic acid 0.5g, beef extract 2.5g, β-glycerophosphate disodium 19g, agar 15g, magnesium sulfate 0.58g, Distilled water 1000mL.

[0030] Each liter of MRS agar medium contains: peptone 10g, yeast extract 5g, beef extract 10g, glucose 20g, sodium acetate 5g, diammoniu...

Embodiment 2

[0034] The morphological identification of Pediococcus pentosaceae R09 of the present invention is carried out according to the following steps: apply the isolated and purified bacterial strains to MRS solid medium respectively, cultivate at 37° C. for 1 to 2 days, and observe the characteristics of the colonies, such as figure 1 As shown, it presents a milky white round colony on the MRS agar medium, the surface of the colony is moist without wrinkles, the edges are neat, and the Gram stain is positive; take a smear of a typical colony, perform Gram staining, and observe under an oil microscope Its bacterial form, such as figure 2 As shown, the thallus of Pediococcus pentosaceae R09 is rod-shaped and has no spores; the morphology of the thallus is observed under a scanning electron microscope, as shown in image 3 as shown, image 3 It is the state when the bacterium of Pediococcus pentosaceae R09 is dividing, and it reproduces by binary fission, and the bacterium is rod-sh...

Embodiment 3

[0042] Properties of Pediococcus pentosaceae R09 of the present invention.

[0043] The Pediococcus pentosaceae R09 of the present invention is carried out the test of producing acid and gas by glucose fermentation, CK is the control group that does not receive Pediococcus pentosaceae R09 in the culture medium, compared with the control group, Pediococcus pentosaceae R09 of the present invention is in MRS liquid On the culture medium, after 3 days of culture at 37°C and 150r / min, the culture medium of Pediococcus pentosaceae R09 turned yellow, indicating that Pediococcus pentosaceae R09 of the present invention produced acid, and a large number of bubbles appeared on the top of the test tube, indicating that Pediococcus pentosaceae R09 R09 produces gas. After the Pediococcus pentosaceae R09 of the present invention was cultivated at 37° C. for 24 hours on the MRS liquid medium, the number of effective viable bacteria was 1.1×10 10 cfu / mL, acid production was 151.8°T.

[0044...

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Abstract

The invention belongs to the technical field of lactic acid bacteria for feed and particularly discloses pediococcus pentosaceus R09 which is preserved in the China General microbiological Culture Collection Center with the preservation No. 12487 on May 23rd, 2016. The strain is a lactic acid bacterium separated from the healthy pig intestinal tract, and is high in safety, capable of generating acid without gas and hydrolyzing arginine and tolerant to common animal drugs. The invention further discloses application of the strain in a microbiological feed additive. By using the prepared microbiological feed additive in daily ration of animals, the number of lactic acid bacteria in the animal body intestinal tract can be increased, the number of Escherichia coli in animal dung can be reduced, the animal body food intake can be improved, and the growth and development of animals can be promoted.

Description

technical field [0001] The invention relates to the technical field of lactic acid bacteria for feed, in particular to a strain of Pediococcus pentosaceae and its application. Background technique [0002] Since the middle of the 20th century, people have gradually added antibiotics to animal feed to prevent the body from contracting diseases, promote rapid growth and development of animals, increase the utilization rate of feed, and increase the output of livestock products, all of which have played a positive role. The disadvantages of the overuse of antibiotics are becoming more and more obvious. For example, the flora in the intestinal tract of animals is in a state of dynamic balance. After long-term consumption of antibiotics, the normal flora of animals will be unbalanced, and after long-term use, it is easy to develop resistance to the body and drugs. Defects such as residues will reduce the body's immunity, thereby reducing the quality of animal products. If people ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/01
CPCC12N1/205C12R2001/01
Inventor 李肖来航线韦小敏李海洋薛泉宏
Owner NORTHWEST A & F UNIV
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