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Acellular tissue matrix composite and preparation method thereof

An acellular tissue and composite material technology, applied in the field of acellular tissue matrix composite materials and their preparation, can solve the problems of difficult regulation of degradation time and three-dimensional structure, poor mechanical strength, loss of active components, etc., to promote adhesion and migration. and proliferation, improved mechanical properties, high biocompatibility

Active Publication Date: 2016-10-12
广州昕生医学材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, when acellular matrix materials are used as repair materials or scaffolds, due to the inherent structure of the material, it is difficult to control the degradation time and three-dimensional structure, and the mechanical strength is poor. In order to adjust the degradation time, the common method is cross-linking, but cross-linking Serious loss of active ingredients after
These problems make it difficult for the material to be applied to multiple clinical indications, and the clinical use is limited, etc.
For example, among the acellular matrix materials, the most in-depth and comprehensive acellular submucosa of the small intestine can guide and support the growth of host cells in vivo, and gradually degrade completely. A natural biological scaffold, which still has the following problems: the submucosa of the small intestine is a single-layer film, which lacks sufficient volume and mechanical support, and it is difficult to achieve a good repair treatment effect, and various soft tissues have various complexities. shape, not a single simple structure can satisfy
The common method to regulate the degradation cycle is direct cross-linking, but the activity will be lost after cross-linking, thus losing the biggest advantage of this material

Method used

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  • Acellular tissue matrix composite and preparation method thereof
  • Acellular tissue matrix composite and preparation method thereof
  • Acellular tissue matrix composite and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Acellular tissue matrix composite material: take the wet weight of 100g of the acellular tissue matrix after decellularization of porcine small intestinal submucosa tissue, crush it, add 100ml sterile water for injection, mix it with 4g hyaluronic acid, pour it into a mold, freeze A porous sheet-like acellular tissue-matrix composite material with a thickness of 2 mm was dried. Weigh 0.2g of NHS and 1.92g of EDC and dissolve in 20ml of 0.1mol / L citric acid / sodium citrate buffer (PH=5.4), add 180ml of ethanol after complete dissolution, and mix well to obtain a cross-linking agent solution. Put into the cross-linking agent solution prepared above for cross-linking for 1 hour, then rinse with sterile water for injection to remove the residual cross-linking agent, and freeze-dry to obtain the acellular tissue matrix composite material.

[0040] The obtained product is used for the surface of the body, is used for soft tissue repair, has the functions of promoting wound hea...

Embodiment 2

[0042] Acellular tissue matrix composite material: Take 100g wet weight of acellular tissue matrix after decellularization of porcine small intestinal submucosa tissue, crush it, add 100ml of 0.01mol / L HCl containing 100mg pepsin (2000~2300U / mg) In the solution, keep constant stirring at room temperature (25°C) for 48 hours, the pH of the viscous solution obtained by enzymatic digestion is about 3.0-4.0, and the pH is raised to 7.4 with NaOH solution, the activity of pepsin is irreversibly inactivated, and the obtained The acellular tissue matrix enzyme digestion solution was evenly mixed with 4 g of sodium alginate, poured into a mold, and freeze-dried to obtain a 2 mm thick porous sheet-like composite material. The scaffold is cross-linked with 0.05 g / ml calcium chloride solution for 10 minutes, washed with sterilized water for injection to remove residual calcium chloride, and freeze-dried to obtain the acellular tissue matrix composite material.

[0043] The obtained produ...

Embodiment 3

[0045] Acellular tissue matrix composite material: Take the wet weight of 100g of the acellular tissue matrix after decellularization of porcine small intestinal submucosa tissue, crush it, add 100ml of sterile water for injection, mix it with 4g of sodium alginate, pour it into a mold, freeze A porous sheet-like acellular tissue-matrix composite material with a thickness of 2 mm was dried.

[0046] The obtained product is used for the surface of the body, is used for soft tissue repair, has the functions of promoting wound healing and promoting blood vessel regeneration, and the degradation time is 1 to 2 weeks. The obtained product is in the shape of a sponge with a micropore size of 20-800 microns and a porosity of 80%.

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Abstract

The invention discloses an acellular tissue matrix composite and a preparation method thereof. The composite is formed by smashing an acellular tissue matrix, adding water or digestive juice for digestion, evenly mixing the product with polyelectrolyte and conducting freezing and drying, wherein the acellular tissue matrix is obtained by conducting acellular implantation on animal tissue. The product is in a porous sponge shape, in-situ implantation induction host vascularization and cell proliferation and growth are achieved with the composite as the support, and therefore tissue healing is promoted, and the composite can be used for tissue repairing such as wound surface repairing, subcutaneous filling and tissue-engineered muscle establishing. By means of the composite, active growth factors are kept, the support with complicated shape and adjustable size can also be established, and the micro-porous size, porosity and degradation time can be adjustable.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a decellularized tissue matrix composite material and a preparation method thereof. Background technique [0002] At present, acellular tissue matrix materials are the materials with the most application potential as tissue repair materials or tissue engineering scaffolds. Many preclinical animal studies and human clinical applications have shown that they are very constructive for remodeling different tissues. promotion. These acellular tissue matrix materials are derived from various animal tissues, including heart valves, blood vessels, skin, nerves, skeletal muscles, tendons, ligaments, small intestinal submucosa (SIS), bladder and liver, etc. The animal source is pig , cattle or sheep. Acellular tissue matrix material is the tissue extracellular matrix that remains after removing the cellular components of the tissue (these components are the main source of ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50A61L27/60A61L27/56A61L27/20A61L27/18A61L27/24A61L27/22
CPCA61L27/18A61L27/20A61L27/222A61L27/24A61L27/3687A61L27/50A61L27/56A61L27/60A61L2430/30A61L2430/34A61L2430/40C08L89/00C08L1/04C08L5/08C08L1/286C08L5/04C08L77/00
Inventor 杨伟红易静楠邓小敏陈大鹏刘江辉
Owner 广州昕生医学材料有限公司
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