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A kind of nano-medicine carrier with magnetocaloric and photothermal effect and preparation method thereof

A nano-drug carrier and photothermal effect technology, which is applied in the field of materials science, can solve the problems such as the lack of therapeutic effect, and achieve the effect of good magnetocaloric effect and good encapsulation effect.

Active Publication Date: 2019-07-23
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex pathogenesis of cancer and the multidrug resistance of tumor cells, conventional chemotherapy often fails to achieve good therapeutic effects

Method used

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  • A kind of nano-medicine carrier with magnetocaloric and photothermal effect and preparation method thereof
  • A kind of nano-medicine carrier with magnetocaloric and photothermal effect and preparation method thereof
  • A kind of nano-medicine carrier with magnetocaloric and photothermal effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The nano-drug carrier provided in this example includes mesoporous silica particles, Fe embedded in the mesoporous silica particles 3 o 4 Nanoparticles and graphene oxide coated on the surface of mesoporous silica particles. The nano drug carrier preparation method comprises the following steps:

[0035] Step 1, 1.5 mmoles of FeCl 3 ·6H 2 O was dissolved in 20 ml of ethylene glycol (EG), and then 7.5 mmol ethylenediamine (EDA) and 0.75 mmol sodium citrate (NaCit) were added to the above solution and sonicated until completely dissolved to obtain a clear solution; then, the clear The solution was transferred to a hydrothermal kettle (polytetrafluoroethylene lined, stainless steel kettle body), at 220 o Cool at room temperature after solvent heat treatment at C for 10 hours; the black precipitate is collected by high-speed centrifugation, washed with ethanol, and dried in vacuum to obtain Fe 3 o 4 nanoparticles.

[0036] Step 2, 0.2 g of Fe 3 o 4 The nanoparticle...

Embodiment 2

[0044] Take 50 mg of the amino-functionalized Fe prepared in Example 1 3 o 4 / mSiO 2 Mesoporous nanoparticles, dispersed in 10 ml of PBS buffer solution of doxorubicin drug (adriamycin concentration 0.5 mg / ml), the above mixture was shaken at room temperature for 24 hours in a light-proof shaker, then centrifuged and washed with PBS buffer , and finally get Fe for storage of doxorubicin drug 3 o 4 / mSiO 2 Mesoporous nanoparticles.

[0045] Add 650 microliters of EDC and 130 microliters of NHS at 2.8 mmol / liter to 20 milliliters of graphene oxide aqueous solution (1 mg / ml) for activation treatment for 1 hour; 3 o 4 / mSiO 2 The mesoporous nanoparticles were added to the activated graphene oxide solution and reacted in the dark for 12 hours; the reddish-brown colloidal particles were collected by high-speed centrifugation, washed with deionized water and freeze-dried to obtain the graphene oxide-coated Fe for doxorubicin storage. 3 o 4 / mSiO 2 mesoporous nanoparticles, ...

Embodiment 3

[0051] Figure 4 The results of cytotoxicity of the nano-drug carriers with magnetocaloric and photothermal effects prepared in Example 1.

[0052] In vitro cytotoxicity was determined using the standard Cell Counting Kit-8 method. Breast cancer cell line 4T1 cell line was purchased from Shanghai Institute of Biochemical Cells and cultured according to the provided method.

[0053]The specific experimental process is as follows: the nano-drug carrier prepared in Example 1 was dispersed in DMEM culture medium and formulated to a concentration of 1 mg / ml. After 4T1 cells were seeded in a 96-well plate (cell density 5000 cells / well), the nano-drug The carrier suspension was immediately added to the 96-well plate, and the final nano drug carrier concentrations were 25, 50, 75, 100 and 200 μg / ml, respectively, and the solution volume was 100 μl.

[0054] After the nano-drug carrier suspension was co-cultured with the cells for 24 hours, 10 microliters of CCK-8 solution was added ...

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Abstract

The invention discloses a nano-drug carrier with the magnetothermal and photothermal effects. The particle size is 50 nanometers to 300 nanometers, and the nano-drug carrier is prepared from mesoporous silica particles, Fe3O4 nanoparticles embedded into the mesoporous silica particles and graphene oxide with which the surfaces of the mesoporous silica particles are coated. The invention further provides a preparation method of the nano-drug carrier. The superparamagnetic Fe3O4 nanoparticles are prepared through a solvothermal method, the Fe3O4 nanoparticles are composited by taking hexadecyl trimethyl ammonium bromide as a structure-directing agent and taking tetraethoxysilane as a silicon source through the sol-gel self-assembling process, and then magnetic mesoporous nanoparticles Fe3O4 / mSiO2 with the magnetic property adjustable and controllable are prepared; the surfaces of the Fe3O4 / mSiO2 mesoporous nanoparticles are coated with graphene through the ion interaction or electrostatic interaction or hydrogen-bond interaction, and then the nano-drug carrier which both can efficiently deliver anti-cancer drugs and has the magnetothermal and photothermal effects is obtained. Accordingly, cancer treatment in which medical chemotherapy cooperates with magnetothermal and photothermal treatment can be achieved.

Description

technical field [0001] The invention belongs to the field of material science and relates to a drug carrier, in particular to a nanometer drug carrier with magneto-thermal and photothermal effects and a preparation method thereof. Background technique [0002] Currently, the high incidence of cancer is a serious problem worldwide. The methods of clinical treatment of cancer at home and abroad are mainly surgery, radiotherapy and drug chemotherapy, among which drug chemotherapy is the most common means in cancer treatment. Due to the complex pathogenesis of cancer and the multidrug resistance of tumor cells, conventional drug chemotherapy often fails to achieve good therapeutic effects. Combination therapy can improve the therapeutic effect of cancer, that is, multiple cancer treatment methods act on the same tumor site at the same time, and the combination of drug chemotherapy and hyperthermia is one of the important methods. [0003] Hyperthermia has been widely accepted ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K41/00A61K45/00A61K47/04A61K47/02A61K31/704A61P35/00
CPCA61K9/5115A61K31/704A61K41/0052A61K45/00
Inventor 朱钰方姚先先
Owner UNIV OF SHANGHAI FOR SCI & TECH
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