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Compound microbial inoculant as well as preparation method and application thereof

The technology of compound microbial inoculum and inoculum is applied in the field of microbial technology treatment of organic waste agricultural resources, which can solve the problems of low reduction of crude fiber content, long fermentation time, unsatisfactory effect, etc. Simple fermentation process and improved carboxymethyl cellulase activity

Inactive Publication Date: 2016-09-28
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some scholars have conducted research on the fermentation process of vinegar lees, but the effect is not satisfactory. First, the decline rate of crude fiber content is relatively low, and the highest decrease rate is only 16.23%. Second, the process is complicated and pretreatment is required. is a long fermentation time

Method used

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  • Compound microbial inoculant as well as preparation method and application thereof
  • Compound microbial inoculant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Culture medium preparation

[0044] PDA medium formula (g / L): potato dipping powder 3.0, glucose 20.0, agar 15.0, natural pH.

[0045] Cha's medium formula (g / L): 30.0 sucrose, 12.0 agar, NaNO 3 3.0, MgSO 4 ·7H 2 O 0.5, KCl 0.5, FeSO 4 ·7H 2 O 0.01, K 2 HPO 4 1.0, natural pH.

[0046] Prepared according to the recipe, boiled, put into a clean Erlenmeyer flask, sealed with a parafilm, tightened with a rubber band, sterilized under high pressure at 121°C for 20 minutes, took it out and put it on a super-clean workbench (turn on ultraviolet sterilization and ventilation), and cool to 40- At 60°C, pour it into a sterile plate (uniform, horizontal, without air bubbles), cool and condense into a solid state, and set aside.

Embodiment 2

[0047] Example 2 Culture of the strain

[0048] Phanerochaete chrysosporium (ACCC 30414) and Trichoderma konshii (CGMCC 3.2878) were activated twice on PDA medium, and then cultured on PDA plate medium at 28°C until a large number of spores were produced (about a week).

[0049] Aspergillus niger (ACCC 30557) and Aspergillus fig (NTG-23) were activated twice on Zapei's medium, and then cultured on Chapei's plate medium at 28°C until a large number of spores were produced (about a week).

Embodiment 3

[0050] Example 3 Preparation of spore suspension

[0051] 1. Elute the spores of the four kinds of bacteria from the plate with sterile physiological saline (0.9% NaCl solution), and obtain the spore liquid of each bacteria respectively (the operation process is aseptic operation).

[0052] 2. Place the obtained spore liquid in a 250mL Erlenmeyer flask (with glass beads) and shake it on a shaker at 150rmp for 4 hours at room temperature to break up and disperse the spores.

[0053] 3. The spore liquid after shaking is filtered with 8 layers of gauze (using a funnel and a glass rod) to obtain a spore suspension.

[0054] 4. Using the method of observing and counting under the microscope of a hemocytometer, adjust the spore suspension of various bacteria to 1×10 6 spores / mL, set aside.

[0055] The spore suspension can also be processed into a dry spore powder inoculum by vacuum freeze-drying technology, so as to be convenient for preservation or packaging for commercial use...

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Abstract

The invention provides a compound microbial inoculant for vinegar residue fermentation; the inoculant comprises mixed fungi, wherein the mixed fungi are formed by mixing phanerochaete chrysosporium, trichoderma koningii, aspergillus niger and aspergillus ficuum. The invention also provides a preparation method of the inoculant and an application of the inoculant in vinegar residue fermentation. With the application of the inoculant, the content of coarse fibers in vinegar residue is reduced, the content of reducing sugar, which is easy to digest in the vinegar residue, is increased, the activity of hydrolase in the vinegar residue is improved and the feed value of the vinegar residue is improved.

Description

technical field [0001] The invention belongs to the field of microbial technology treatment of organic waste for agricultural use, and relates to a compound microbial bacterial agent capable of promoting the fermentation of vinegar grains and a preparation method thereof. Background technique [0002] Vinegar grains are residues produced during the solid-state fermentation of vinegar brewing with starchy raw materials as the main ingredient. The annual output of vinegar in my country is 2 to 2.5 million tons, and it is increasing at a rate of 7% every year. According to the calculation of 0.6-0.7 tons of vinegar grains produced by producing 1 ton of standard solid-state fermentation secondary vinegar, the annual output of vinegar grains in my country is 1.20-1.75 million tons. Such abundant and low-cost vinegar residue resources have not been fully and rationally utilized at present, which not only causes waste of resources but also causes environmental pollution. Except f...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23K10/18C12R1/645C12R1/885C12R1/685C12R1/66
CPCC12N1/14
Inventor 董晓芳佟建明崔耀明
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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