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Bio-fertilizer for efficient degradation of atrazine and preparation method thereof

A technology of atrazine and bio-fertilizer, applied in the field of bio-fertilizer, can solve problems such as inability to grow, loss of degradable atrazine activity, unstable degradation performance, etc., to increase organic matter content, improve field adaptability, and improve degradation The effect of stability

Active Publication Date: 2016-09-28
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, Acinetobacter DNS32, strain M91-3, Pseudomonas ADP, FR, etc. can completely degrade atrazine under artificial culture conditions, but in complex farmland environments, the strains generally grow slowly and have poor degradation performance. Stability and other issues
Pushing its reason, on the one hand, it may be that changes in environmental conditions (such as pH, moisture content, temperature, organic matter content) directly affect the activity of microorganisms to degrade atrazine; The influence of inhibitors, or the influence of the indigenous flora in the environment, or even the inability to grow due to antagonism, etc., these may cause the inoculated microorganisms to fail to become dominant bacteria and lose the activity of degrading atrazine
Therefore, the current atrazine biodegradation studies mostly stay at the laboratory level, and there is no commercial atrazine biodegradation agent on the market.

Method used

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  • Bio-fertilizer for efficient degradation of atrazine and preparation method thereof
  • Bio-fertilizer for efficient degradation of atrazine and preparation method thereof
  • Bio-fertilizer for efficient degradation of atrazine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Preparation of fermented bacterial fertilizer

[0029] 1. Activation of Bacillus subtilis and Bacillus licheniformis

[0030] Add the freeze-dried powder of Bacillus subtilis (the number of viable bacteria ≥ 100 billion / g) into the sterilized LB liquid medium at an amount of 1%, and place it in a shaker at 30°C with a rotation speed of 180 rpm Activation culture for 12 hours per minute; the activation of Bacillus licheniformis is the same as that of Bacillus subtilis;

[0031] LB liquid medium: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH 7.0;

[0032] 2. Activation of Lactobacillus plantarum and Lactobacillus rhamnosus

[0033] Add the freeze-dried powder of Lactobacillus plantarum (the number of viable bacteria ≥ 100 billion / g) into the sterilized MRS liquid medium at an amount of 1%, and culture it statically at 37°C for 12 hours, and the measured OD value is 0.5; then transfer once again, when the OD value reaches 0.5 again; press 10...

Embodiment 2

[0040] Embodiment 2 prepares biological fertilizer

[0041]1. Activation of fermentation strains

[0042] Two bacterial strains, BTAH1 (preservation number: CGMCC No.1113) and Graninella strain HBZA0511 (preservation number: CGMCC No.1801), are used;

[0043] Add the strain BTAH1 of the genus Exiguobacterium into the activation medium (1 / 10 of the LB medium), rotate at 120 rpm at 30°C, and cultivate on a shaker for 24 hours. After the OD value reaches 0.5, obtain the seed liquid and set aside; The activation of Graninella strain HBZA0511 is the same as that of Exiguobacterium strain BTAH1;

[0044] 1 / 10 LB medium: every 1000ml contains 0.5g yeast extract, 1g peptone and 1g sodium chloride.

[0045] 2. Strain fermentation: the seed liquid is transferred to the fermenter according to the inoculum amount of 10% by mass ratio (the seed liquid of the two kinds of bacteria is respectively pressed by 5% consumption); the fermentation temperature is 30 ° C, and the stirring speed is...

Embodiment 3

[0059] Embodiment 3: Preparation of fermented bacterial fertilizer

[0060] 1. Activation of Bacillus subtilis and Bacillus licheniformis

[0061] With embodiment 1.

[0062] 2. Activation of Lactobacillus plantarum and Lactobacillus rhamnosus

[0063] With embodiment 1.

[0064] 3. Fermentation material preparation

[0065] Take the corn plants and break off the corn, harvest the corn, dry it and crush it with a grinder, the crushing fineness is between 2mm-3mm, and obtain corn stalks; the cottonseed cake, wheat bran, corn stalks, and fish bone meal are separated according to the quality Mix at a ratio of 9:9:48:1, sterilize evenly at 80°C for 20 minutes, dry in the sun, and use it as a fermentation material for later use;

[0066] 4. Fermentation

[0067] Add the expanded fermentation liquid of Lactobacillus plantarum and Lactobacillus rhamnosus to the fermented materials after sterilization and drying according to the inoculation amount of 2.8%, and then add the activa...

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Abstract

The invention discloses bio-fertilizer for efficient degradation of atrazine and a preparation method thereof. Wheat straws, plant ash, saw dust and self-made zymophyte fertilizer beneficial for strain survival and growth are compounded and the compound, Exiguobacterium strain BTAH1 and Shinella granuli HBZA0511 are mixed and are fermented to form the bio-fertilizer. The self-made zymophyte fertilizer is prepared from nutrition bases such as cake, wheat bran, corn straws and fish bone powder, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum and lactobacillus rhamnosus through fermentation. The bio-fertilizer is sowed in the field so that soil atrazine herbicide residual quantity is fast reduced by 95% or more, is conducive to soil bioremediation, is suitable for green agricultural product production and improves soil organic content.

Description

technical field [0001] The invention relates to a biological fertilizer for efficiently degrading atrazine and a preparation method thereof, belonging to the technical field of biological fertilizers. Background technique [0002] Atrazine is a widely used triazine herbicide, which is mainly used in corn fields. More than half of the corn fields in my country use atrazine for weeding. Due to the long duration of atrazine, atrazine remains in the soil for a long time during long-term use, and its residues are likely to cause toxicity to some subsequent crops (such as wheat, rice, soybean, etc.). It has a long retention period in water and soil and is bioaccumulative. In addition, it can cause a certain degree of damage and pollution to the ecological environment, thereby threatening the health of animals and humans. Biodegradation is the main way to remove residual atrazine pollution in soil. Due to its fast degradation speed, low cost, good effect, and no secondary pollutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05F17/00A01C21/00C05G3/80
CPCA01C21/005C05D1/00C05F1/002C05F17/00C05G3/80C05F5/002C05F11/00C05F11/08C05F9/04Y02W30/40
Inventor 刘孝永周庆新赵双枝陈相艳辛雪陈蕾蕾孙华张翔
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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