Compound biological enzyme preparation, compound microbial agent and application thereof in treatment of oily sludge
A composite microbial inoculum and composite biological enzyme technology, applied in biological water/sewage treatment, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the complex process, difficult to remove other harmful components of oily sludge, It takes a long time to deal with problems, and achieves the effect of broad application prospects, low cost, and no secondary pollution.
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[0047] The present invention will be further described by means of examples below, but the present invention is not limited to such examples.
Embodiment 1
[0049] 1. Purpose of the test:
[0050] Study the separation effect of compound biological enzyme preparation on crude oil and mud in oily sludge, and use compound microbial agent to further treat oil sludge to achieve crude oil recovery and oil sludge harmless treatment.
[0051] 2. Test material:
[0052] 2.1 Oily sludge: CNOOC sludge.
[0053] 2.2 Preparation of compound biological enzyme preparation
[0054] Bacillus subtilis, Bacillus pumilus, Candida Albicans, Lactobacillus breris and their medium were purchased from Xi'an Zirui Biotechnology Co., Ltd. Among them, beef extract peptone medium is used to cultivate Bacillus subtilis and Bacillus pumilus, potato medium is used to cultivate Candida, and MRS medium is used to cultivate Lactobacillus brevis. The composition of the culture medium is as follows:
[0055] Beef extract peptone medium (g / L): beef extract 3, peptone 10, NaCl 5 (1.5% agar should be added to the solid medium);
[0056] Potato medium (g / L): potato ...
Embodiment 2
[0079] 1. Purpose of the test
[0080] PetroChina Safety and Environmental Protection Research Institute and Huabei Oilfield jointly verified the feasibility of promoting the construction process of oily sludge and the effect of harmless treatment of oily sludge by the comprehensive treatment technology of oily sludge compound biological enzyme and microbial method.
[0081] 2. Test materials
[0082] 2.1 Huabei oilfield oil sludge;
[0083] 2.2 Preparation of compound biological enzyme preparation
[0084] The composite biological enzyme preparation prepared in Example 1 was adopted.
[0085] 2.3 Preparation of composite microbial agent.
[0086] The composite microbial inoculum prepared in Example 1 was adopted.
[0087] 3. Test steps
[0088] In this experiment, the amount of oily sludge treated in each batch is 5t (ton).
[0089] (1) The test is carried out in two steps:
[0090] a. Use compound biological enzyme preparation to separate and elute crude oil, asphalt,...
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