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System for diagnosing helicobacter pylori infection of human body based on spectrophotometer and method for detecting content of ammonia gas in expired gas of person

A spectrophotometer and Helicobacter pylori technology, applied in the field of medical detection, can solve the problems of high detection cost, time-consuming and labor-intensive, low detection cost, etc., and achieve the effect of reducing detection cost, easy to accept, safe and accurate diagnosis

Inactive Publication Date: 2016-09-21
长沙三相医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] At present, the existing Hp detection methods are divided into invasive and non-invasive diagnostic methods. The invasive diagnostic methods mainly rely on gastroscopy biopsy, which requires patients to endure the great pain caused by gastroscopy and is time-consuming and laborious; non-invasive detection methods such as 13 C-UBT or 14 C-UBT method, the former has no radioactive pollution, but the detection requires a mass spectrometer, and the detection cost is high; the latter detection 14 C is radioactive, and the detection cost is low, but it has certain radioactivity, so it should not be used in children and pregnant women; therefore, it is urgent to develop a method that has no side effects, simple operation, warm conditions, long service life and can accurately detect Helicobacter pylori infection. method

Method used

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  • System for diagnosing helicobacter pylori infection of human body based on spectrophotometer and method for detecting content of ammonia gas in expired gas of person
  • System for diagnosing helicobacter pylori infection of human body based on spectrophotometer and method for detecting content of ammonia gas in expired gas of person
  • System for diagnosing helicobacter pylori infection of human body based on spectrophotometer and method for detecting content of ammonia gas in expired gas of person

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Test object: subject 1, female, 38 years old, experienced 13 C-urea breath test was positive.

[0048] 2. Detection method:

[0049] (1) Subjects are required to fast on an empty stomach or fast more than two hours in advance.

[0050] (2) The subject exhales into the sampling bag until it is filled and marked as a blank sample.

[0051] (3) Take the kit and mix it with warm boiled water for ordinary urea medicament.

[0052] (4) After 30 minutes, the subject exhaled again into the sampling bag until it was filled with the marked sample.

[0053] (5), pass the gas in the sampling bag marked with blank sample and sample into ammonia capture agent 3mL 5mMH 2 SO 4 Solution, chromogenic agent 0.3mL salicylic acid-potassium sodium tartrate solution (concentration of salicylic acid is 50g / L, concentration of potassium sodium tartrate is 50g / L), 0.1mL sodium nitroferricyanide solution (concentration is 10g / L) and 0.1mL sodium hypochlorite solution (0.05mol / L) were ad...

Embodiment 2

[0061] 1. Test object: subject 2, male, 56 years old, experienced 13 C-urea breath test was negative.

[0062] 2, detection method: the detection method of the present embodiment is exactly the same as that in the last embodiment, and its detection result is as table 2:

[0063] Table 2 The detection data of ammonia in the blank sample and sample exhaled by subject 2

[0064]

[0065] 3. Conclusion: It can be seen from Table 2 that the content of ammonia in the exhaled breath of the subject before and after taking the medicine is basically the same and is at a lower level after being accurately calculated by the standard curve method.

Embodiment 3

[0067] 1. Test object: subject 3, female, 32 years old, experienced 13 C-urea breath test was negative.

[0068] 2, detection method: the detection method of the present embodiment is exactly the same as that in the last embodiment, and its detection result is as table 3:

[0069] Table 3 Test data of ammonia in blank sample and sample exhaled by subject 3

[0070]

[0071] 3. Conclusion: It can be seen from Table 3 that the content of ammonia in the exhaled breath of the subject before and after taking the medicine is basically the same and is at a lower level after being accurately calculated by the standard curve method.

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Abstract

The invention discloses a system for diagnosing helicobacter pylori infection of a human body based on a spectrophotometer and a method for detecting the content of ammonia gas in expired gas of a person. The system comprises an expired gas diagnostic kit and the spectrophotometer. The interior of the expired gas diagnostic kit is provided with three reagent pools which are respectively provided with a urea electuary, an ammonia capturing agent and a color developing agent. The method for detecting the content of ammonia gas in the expired gas of a person comprises the following steps: collecting the expired gas of the person; absorbing and immobilizing ammonia gas in the expired gas by using the ammonia capturing agent; and determining the content of the immobilized ammonia gas by using the spectrophotometer. The detection system and method can accurately and rapidly detect the content of ammonia gas in the expired gas of a person, so accurate high-efficiency diagnosis of helicobacter pylori infection of the person can be realized; moreover, the detection system and method reduce production cost for reagents, eliminate the hidden trouble of toxic and side effect of conventional reagents and can be promoted and applied.

Description

technical field [0001] The invention relates to a detection system for diagnosing human Helicobacter pylori infection and a method for detecting ammonia content in human exhalation; in particular, it relates to a human body taking ordinary urea granules, which are released through the action of urea and human Helicobacter pylori marker urease Ammonia gas, and then use the ammonia capture agent to capture the ammonia gas in the sample, and use the spectrophotometric method to detect the detection system and method of fixed ammonia gas; the degree of human Helicobacter pylori infection is diagnosed by the ammonia content in the human breath, which belongs to medical treatment detection technology field. Background technique [0002] Helicobacter pylori (abbreviated as Hp) is one of the most common pathogens in the world. In China, the infection rate of Helicobacter pylori in young adults is about 30%, and the infection rate in people over 50 years old is 50% to 80%. The detec...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/22
CPCG01N1/22G01N21/31
Inventor 纪效波周文强罗磊丁莹莹李丽娟
Owner 长沙三相医疗器械有限公司
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