Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rhodosporidium toruloides for producing linoleic acid and linolenic acid and preparing method thereof

A technology of Rhodosporidium toruloides, which is applied in the field of production of linoleic acid and α-linolenic acid, can solve the problems of increasing the degree of Rhodosporidium toruloides and achieve the effect of improving the utilization value

Active Publication Date: 2016-09-21
FUJIAN MINDONG REJUVENATION PHARMA
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problem that the existing technology cannot greatly improve the production of linoleic acid and α-linolenic acid by Rhodosporidium toruloides ACCC 20341, the inventors of the present application have found that the intracellular clone The obtained Δ12 desaturase gene showed high activity when expressed in Rhodosporidium toruloides. It was connected to the plant binary expression vector pCAMBIA1300, transformed into Agrobacterium tumefaciens LBA4404 cells, and Δ12 The desaturase gene was transformed into Rhodosporidium toruloides to realize the overexpression of Mortierella alpina Δ12 desaturase with high activity in Rhodosporidium toruloides cells, thereby enhancing the production of linoleic acid and Alpha-linolenic acid capacity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rhodosporidium toruloides for producing linoleic acid and linolenic acid and preparing method thereof
  • Rhodosporidium toruloides for producing linoleic acid and linolenic acid and preparing method thereof
  • Rhodosporidium toruloides for producing linoleic acid and linolenic acid and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] Preferably, the preparation method of Rhodosporidium toruloides of the present invention includes the step of transforming Rhodosporidium toruloides with Agrobacterium tumefaciens. Preferably, the transformation method includes a method of mixing and co-cultivating two bacterial liquids to perform transformation.

[0043] Preferably, the preparation method of Rhodosporidium toruloides of the present invention further includes a step of verifying recombinants obtained after transformation of Rhodosporidium toruloides by Agrobacterium tumefaciens. The verification method can adopt any method commonly used in the art, including but not limited to, PCR amplification verification method, enzyme digestion verification method, immunological verification method, and enzyme activity verification method. From the perspective of operation method and cost saving, the PCR amplification verification method is preferred. Specifically, the genome of Rhodosporidium toruloides after co-...

Embodiment

[0045] 1. Strains and experimental materials

[0046] Rhodosporidium toruloides ACCC 20341 and Mortierella alpina were purchased from China Agricultural Biological Culture Collection and Management Center, and Agrobacterium tumefaciens LBA4404 was purchased from Protin Biotechnology (Beijing) Co., Ltd. company. The plasmid and genome extraction kit were purchased from TIANGEN Company, the vector pCAMBIA1300 was purchased from Biovector Company, the Easy Pfu DNA polymerase was purchased from Quanshijin Company, and the nylon membrane Hybond-N+ membrane was purchased from Amersham Company. Restriction enzymes were purchased from NEB Company. Chemical reagents were purchased from Sinopharm Chemical Reagent Co., Ltd. and were of analytical grade.

[0047] 2. Construction of Δ12 desaturase gene expression vector pCAMBIA-FAD12:

[0048] 1) The Mortierella alpina strain and the Rhodosporidium toruloides strain were respectively inoculated in 50 mL Erlenmeyer flasks containing YEPD...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses rhodosporidium toruloides for producing linoleic acid and linolenic acid and a preparing method thereof. High-activity delta 12 desaturase gene obtained through intracellular cloning in Mortierella alpine is connected to an improved plant binary expression vector pCAMBIA1300 and transferred into Agrobacterium tumefaciens LBA4404 cells, the delta 12 desaturase gene is combined into a genome of rhodosporidium toruloides through co-culture to achieve overexpression of the Mortierella alpine delta 12 desaturase in rhodosporidium toruloides cells, and therefore the capacity for producing linoleic acid and alpha-linolenic acid through rhodosporidium toruloides can be enhanced.

Description

technical field [0001] The invention relates to the field of production of linoleic acid and α-linolenic acid, in particular to a Rhodosporidium toruloides producing linoleic acid and α-linolenic acid and a preparation method thereof, and simultaneously producing linoleic acid and α-linolenic acid Methods. Background technique [0002] Linoleic acid (cisΔ9,12) is an essential fatty acid for the human body, which cannot be synthesized by the human body and can only be ingested from food. Linoleic acid can significantly reduce the content of cholesterol in the blood, thereby preventing the risk of atherosclerosis. Linoleic acid has a special effect of lowering blood lipids, blood pressure, and promoting microcirculation in the body, which can prevent or reduce the incidence of cardiovascular diseases, especially for the prevention and treatment of vascular atherosclerosis, angina pectoris, coronary heart disease, and senile obesity. extremely beneficial. At the same time, l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/19C12N15/81C12P7/64
CPCC12N9/0071C12N15/81C12N2800/102C12N2800/60C12P7/6427C12Y114/19006
Inventor 祁峰张明亮孙磊江贤章黄建忠陈有为
Owner FUJIAN MINDONG REJUVENATION PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com