A method of chemically inducing corn parthenogenesis

A technology of parthenogenesis and operation method, which is applied in the field of corn breeding to achieve the effects of fast processing speed, avoiding the flow of medicinal liquid and convenient use.

Inactive Publication Date: 2018-06-15
SHENYANG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The technical problems to be solved by the present invention are: (1) to solve the problem of safe use of chemical induction treatment agents, no matter using highly toxic reagent colchicine or herbicide induction, safe operation can be realized; (2) Simplify the treatment method, Reduce breeding procedures and improve processing efficiency; (3) Completely solve the problem of pollen pollution; (4) Realize large-scale application

Method used

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  • A method of chemically inducing corn parthenogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Operation method for inducing maize parthenogenesis

[0049] (1) Preparation of inducer

[0050] Dilute colchicine (COL) with water to a concentration of 0.15% inducer.

[0051] (2) Treatment of test materials

[0052] The treatment object is maize hybrid 'Xianyu 335'.

[0053] (3) Ear treatment

[0054] The tool is an improved needle-nose pliers (see figure 1 ): Use needle-nose pliers with a specification of about 6″~7″. Use a grinding wheel to grind the side of the needle-nose pliers without a cutting edge, and extend the cutting edge part B of the jaw to make a jaw with a single-sided cutting function. At the same time, slightly smooth the cutting edge on one side of the jaws to form a gap A of 1 mm to 2 mm when the jaws are closed, and blunt the toughness for cutting corn filaments.

[0055] The ears are strictly bagged before silking. On the 4th day after the filaments spit out, take off the paper bag, grab the filaments with the left hand, hold th...

Embodiment 2

[0060] Example 2 Operation method for inducing parthenogenesis in waxy maize

[0061] (1) Preparation of inducer

[0062] Colchicine and dimethyl sulfoxide (DMSO) sold on the market were selected and diluted with water to form an inducer of 0.2% colchicine + 2% dimethyl sulfoxide.

[0063] (2) Treatment of test materials

[0064] The object of treatment is the waxy corn hybrid 'Shen Nuo 3'.

[0065] (3) Ear treatment

[0066] The ears are strictly bagged before silking. 4 days after the filaments spit out, take off the paper bag, grab the filaments with your left hand, hold the improved needle-nose pliers in your right hand, and use the needle-nose pliers to pinch the ear buds at the top of the ear buds and close to the young ears of the ears. When the filaments in the bracts have been cut off. Then slowly pull out the filament by hand, leaving a hole.

[0067] (4) Inject the inducer

[0068] Inject about 3ml of the inducer into the hole with a syringe, and it is advisa...

Embodiment 3

[0071] Example 3 Operation method for inducing sweet corn parthenogenesis

[0072] (1) Preparation of inducer

[0073] Select colchicine, dimethyl sulfoxide and cyanine (MH) sold on the market, and dilute with water to form 0.2% colchicine + 2% dimethyl sulfoxide + 40mg·kg-1 cyanogen inducer.

[0074] (2) Treatment of test materials

[0075] The object of treatment is sweet corn hybrid "Shentian 6".

[0076] (3) Ear treatment

[0077] The ears are strictly bagged before silking. 4 days to 5 days after the filaments are spit out, take off the paper bag, grab the filaments with your left hand, hold the improved needle-nose pliers in your right hand, and pinch the ear with the needle-nose pliers at the top of the ear bud about 4cm down and close to the young ear of the ear. Flower buds, when you feel that the filaments in the bracts have been cut off. Then slowly pull out the filament with your hands to form a hole.

[0078] (4) Inject the inducer

[0079] Use a syringe t...

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Abstract

The invention provides a chemical induction corn parthenogenesis operation method. The chemical induction corn parthenogenesis operation method is characterized by comprising strictly bagging female ears before filaments are protruded, dropping off paper bags in 4-5 days after the filaments of the female ears are protruded, pinching the filaments under bract isolation conditions by the aid of improved long-nose pliers, pulling out the filaments by hands and reserving a hole after each filament is pulled out; injecting preferably 2-5 ml of inductive agents into each hole by the aid of a syringe; sleeving the female ears with the paper bags to complete treatment procedures. The chemical induction corn parthenogenesis operation method has the advantages that the problems in the aspect of safe use of poisonous reagents can be solved, the problem of existing pollen pollution can be thoroughly solved, and operation procedures are carried out under completely open conditions; good chemical application effects can be realized, operation can be carried out in all seasons, and the chemical induction corn parthenogenesis operation method is easy to implement, high in treatment speed and suitable for large-scale application.

Description

technical field [0001] The invention belongs to the technical field of corn breeding, in particular to an operation method for chemically inducing parthenogenesis in corn. Background technique [0002] The conventional method of breeding maize inbred lines requires at least 7 generations of continuous selfing to breed an inbred line, and even more than 10 generations for materials with complex genetic backgrounds. In order to speed up the breeding speed of maize inbred lines, shorten the breeding cycle, and improve the purity of inbred lines, chemical induction methods and genetic induction methods are usually used at home and abroad: [0003] 1. Colchicine induction method [0004] Utilizing the principle that colchicine can prevent cell division and result in chromosome doubling, maize parthenogenesis is induced with colchicine inducer. The main method is to smear the filaments with a certain concentration of colchicine during the silking stage of corn, so that it can di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 史振声李凤海钟雪梅朱敏王志斌张喜华
Owner SHENYANG AGRI UNIV
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