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Mushroom bacterial strain preparation method

A technology for strain preparation and shiitake mushrooms, applied in botany equipment and methods, mushroom cultivation, fertilizer mixture, etc., can solve the problems of long germination period of branch strains, time-consuming and labor-intensive seeding of sawdust, low incidence of strains, etc. Achieve the effects of inhibiting the growth of miscellaneous bacteria, prolonging the shelf life, and reducing the incidence rate

Inactive Publication Date: 2016-09-21
山东福国菌业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for preparing shiitake mushroom strains, which solves the problems of time-consuming and labor-intensive sawdust species, high pollution rate, long germination period of branch strains, and late fruiting, and the incidence of strains is low.

Method used

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Examples

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Embodiment 1

[0021] A preparation method of shiitake mushroom strain:

[0022] (1) Choose Amorpha fragrans branches with a diameter of 0.5 cm and a length of 6 cm, boil them in boiling water for 20 minutes, put them into a pool containing chondroitin sulfate waste liquid and soak for 36 hours, and the ratio of chondroitin sulfate waste liquid to water in the pool is 1 : 10, chondroitin sulfate waste liquid is the waste liquid that enzymatic method produces chondroitin and produces, contains polysaccharide 200mg / L, amino acid 5000mg / L, salt 20mg / L, rinses, drains, and keeping branch moisture is 50%;

[0023] (2) Put the branches, bran and water in a mass ratio of 4:1:0.2, put them into a 17cm*36cm folding bag, stir evenly, the branches are covered with bran, and sterilize under the conditions of 0.15Mpa, 120 Sterilize at ℃ for 2 hours;

[0024] (3) Inoculate grain strains to 7% of the weight of the branches, and after the branches are covered with mycelium, continue to cultivate for 20 day...

Embodiment 2

[0028] A preparation method of shiitake mushroom strain:

[0029] (1) Choose oak branches with a diameter of 0.5 cm and a length of 8 cm, put them in a pool and boil them for 30 minutes, then soak them in a pool containing chondroitin sulfate waste liquid for 36 hours. The ratio is 1:50, the chondroitin sulfate waste liquid is the waste liquid produced by enzymatic hydrolysis of chondroitin, containing 500mg / L of polysaccharide, 10000mg / L of amino acid, 50mg / L of salt, rinsed, drained, and the water content of the branches is kept at 60%;

[0030] (2) Put the branches, bran and water in a mass ratio of 4:1:0.2, put them into a 17cm*36cm folding bag, stir evenly, the branches are covered with bran, and sterilize under the conditions of 0.15Mpa, 120 Sterilize at ℃ for 3 hours;

[0031] (3) Inoculate grain strains to 7% of the weight of the branches, and after the branches are covered with mycelium, continue to cultivate for 30 days;

[0032] (4) Oak sawdust, sweet-scented osma...

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Abstract

The invention relates to a mushroom bacterial strain preparation method comprising the following steps: selecting a branch of 0.5 cm in diameter and 6-8cm in length, boiling, immersing, washing and draining; evenly mixing the branch with wheat bran and water, and disinfecting the mixture; inoculation; inoculating the branch onto a culture bag, thus forming a bacterial strain material bag after spawn running. The culture bag comprises the following materials (wood chip, sweet osmanthus, daun ngokilo, Sedum aizoon L, wheat bran, cane sugar and gypsum) according to the proportion of 28: 10-15: 10: 5:10:10:20-25:1:1, wherein the water content is 54%, and the weight is 2kg / bag. A conventional method is time wasting and labor wasting in sawdust bacterial strain mode, and high in pollution rate; the branch bacterial strain is long in spawn running period, and late in fruiting; the mushroom bacterial strain preparation method can solve said problems, and is low in bacterial strain disease incidence.

Description

technical field [0001] The invention relates to a cultivation method of strains, in particular to a method for preparing mushroom strains. Background technique [0002] At present, there are two types of cultivars used in the production of bagged shiitake mushrooms: solid species and liquid species. Now mainly solid species, solid species mainly include sawdust species and branch species, each has its own strengths, but also has disadvantages. [0003] Disadvantages of sawdust seed: due to the poor control of moisture when mixing materials, the mycelia are weak when the strain is wet, which makes it difficult to break the block, time-consuming and labor-intensive, and the sawdust seed prolongs the exposure time of the inoculation hole during the process of breaking the block. The pollution rate is high, causing great distress and loss to the growers. [0004] Disadvantages of branch species: the currently used branch species are thicker and shorter, thicker will increase t...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
CPCA01G18/00C05D3/00C05G3/00
Inventor 王克振李兆宝黄秀珍
Owner 山东福国菌业有限公司
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