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Multi-layer electrospun membrane and use thereof

A technology of electrospinning membrane and electrospinning, applied in medical science, prosthesis, etc., can solve the problems of unfavorable cell and tissue growth, lack of biological activity, and inability to degrade naturally.

Inactive Publication Date: 2016-09-07
王宇飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that these inert materials cannot be degraded naturally, which may lead to granulation tissue formation and induce chronic irritation resulting in long-term foreign body reactions.
In recent years, more and more artificially synthesized degradable materials have been used in the research of dural replacement materials, such as polylactic acid (PLA), polycaprolactone (PCL), polyhydroxybutyrate (PHB), polypropylene glycol (PPG), etc. , these materials can be naturally degraded in the body, thereby avoiding the risk of infection and immune response caused by long-term storage [26-33], these degradable materials continue the good mechanical properties and tissue compatibility of artificial inert materials, and can maintain sufficient Its strength is convenient for operation and prevention of cerebrospinal fluid leakage, but as a high molecular polymer product, the disadvantage is that it lacks biological activity, and has no obvious promotion effect on wound healing and dura mater regeneration
Moreover, the hydrolyzed products of these polymers will produce a local slightly acidic environment, which is not conducive to the growth of cells and tissues.

Method used

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  • Multi-layer electrospun membrane and use thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0102] 1) Preparation of the inner layer material: the inner layer material is PLA, the spinning solution concentration is 5%wt, the electrospinning parameters are flow rate of 1.0ml / h, voltage of 12kV, receiving distance of 15cm, and electrospinning for 2h;

[0103] 2) Preparation of the middle layer material: the middle layer material is selected from PLA / PCL (1 / 1) group, the composition is 50% PLA50% PCL, the concentration of the spinning solution is 5% wt, and the electrospinning parameters are flow rate of 1.0ml / h, voltage of 12kV, Receiving distance 15cm, electrospinning 4h;

[0104] 3) Preparation of the outer layer material: the outer layer material is selected from the collagen / PLA (4 / 1) group, the composition is 80% collagen 20% PLA, the concentration of the spinning solution is 6% wt, and the electrospinning parameters are flow rate 1.2ml / h, voltage 14kV, receiving distance 15cm, electrospinning 6h.

[0105] Further, the electrospinning conditions are an indoor tem...

Embodiment 1

[0118] Subcutaneous implantation experiment of electrospun membrane inner and outer layers in SD rats

[0119] Male SD rats, with a body weight of about 200-300 g, numbered 12, were housed in separate cages, fed with feed provided by the Experimental Animal Center, and drank water freely. After observing for 3 days without abnormality, the experiment was started.

[0120] All experimental rats were anesthetized with intraperitoneal injection of 300 mg / kg chloral hydrate solution, the back was shaved, and routine aseptic treatment was performed. An incision parallel to the midline with a length of 3 cm and a depth of subcutaneous was made at each symmetrical part of the back midline. The inner layer material and the outer layer material were respectively implanted on the left and right sides of each rat's back.

[0121] After the operation, they were bandaged with sterile gauze, and the external dressing was changed every day. They were kept in separate cages for routine feedi...

Embodiment 2

[0126] Implantation of multilayer electrospun membranes into dura mater defects in New Zealand rabbits

[0127] Six New Zealand rabbits, male and female randomly, weighing 2-3 kg, were housed in separate cages for 7 days in quarantine, maintained the ambient temperature and fed regularly with the feed provided by the Experimental Animal Center.

[0128] Use 3% pentobarbital sodium 70mg / kg body weight to inject anesthesia into the rabbit's ear vein, fix the head on the animal operating table, fix the head with a bandage, infuse fluid during the operation and intubate to allow breathing, disinfect with 2% iodine and 75% alcohol . A 4cm long straight incision was made on the midline to cut the scalp up to the skull and expose the skull. On the posterior side of the coronal line, a cranial drill drills a bone window with a diameter of about 12 mm to expose the dura mater. A circular dural and arachnoid defect of about 8mm×8mm was excised.

[0129] The multi-layer electrospun memb...

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Abstract

The invention relates to the technical field of nano-biomaterials, in particular to a multi-layer electrospun membrane and the use thereof. The multi-layer electrospun membrane includes an inner layer material, an intermediate layer material and an outer layer material. The multi-layer electrospun membrane is used as a dura mater substitute material, and has significantly improved performances as compared with a collagen matrix through comparison tests on mechanical properties, porosity, water absorption and water repellency.

Description

technical field [0001] The invention relates to the technical field of nanobiological materials, in particular to a multilayer electrospun membrane and its application. Background technique [0002] 1. Research status of dura mater substitute materials [0003] The dura mater is the outermost layer of the membrane structure wrapped around the brain and spinal cord. It is a relatively strong connective tissue film. The inner surface of the natural dura mater structure is a single layer of fibroblasts with a dense structure, which is thin and smooth; the middle Layer contains more fibroblasts and a small amount of collagen; outer layer is thicker, contains more collagen components and a small amount of fibroblasts. The inner surface of such a structure is smooth and flat, and the whole is very tough, which can well protect the brain and prevent the leakage of cerebrospinal fluid. Due to traumatic brain injury, brain tumor and cerebrovascular disease, the dura mater must be c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/00A61F2/02
CPCA61F2/0063A61F2/02A61F2210/0076
Inventor 王宇飞郭洪峰应大君
Owner 王宇飞
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