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Method for detecting aluminum ions by using reactive dye fluorescence probe

A technology of fluorescent probes and reactive dyes, applied in the field of functional reactive dyes and their preparation, can solve the problems of less aluminum ions, no detection range and detection limit of aluminum ions, etc., and achieve good selectivity, low cost, and easy use effect of effect

Inactive Publication Date: 2016-08-31
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] After reviewing Chinese patents, it was found that there are very few patents using fluorescence spectroscopy to detect aluminum ions, and there is no patent indicating the relationship between the probe and the concentration of aluminum ions, let alone the detection range and detection limit of aluminum ions

Method used

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  • Method for detecting aluminum ions by using reactive dye fluorescence probe
  • Method for detecting aluminum ions by using reactive dye fluorescence probe
  • Method for detecting aluminum ions by using reactive dye fluorescence probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the synthesis of active dye fluorescent probe

[0034] (1) Synthesis of rhodamine B hydrazide

[0035] Take a 100mL three-neck flask, weigh 1.200g (2.5mmol) Rhodamine B and dissolve it in 30mL absolute ethanol, stir vigorously at room temperature and slowly add 1mL (19.8mmol excess) 98% hydrazine hydrate dropwise. Heat to reflux at 78°C for 2 hours until the solution turns from dark purple to clear yellow. After the reaction is completed, cool to room temperature, and use a rotary evaporator to evaporate the solvent and excess hydrazine hydrate to obtain a light yellow crude product of rhodamine B hydrazide. Take 50 mL of newly prepared 1M HCl and add it to the crude product, and the product dissolves into a pink solution. Slowly add 1M NaOH to the solution under stirring to adjust the pH value to between 9-10. When NaOH was added dropwise to the mixed solution, when the pH of the solution was adjusted to about 6, pale pink flocculent precipitates began...

Embodiment 2

[0047] Configure 8×10 -6 M aluminum nitrate nonahydrate is used as the aluminum ion test solution, and the reactive dye fluorescent probe synthetic in the application embodiment 1 detects the method for the aluminum ion wherein, and concrete steps are:

[0048] Step 1: Dissolve the reactive dye fluorescent probe synthesized in Example 1 in the solvent DMF, and use the solvent DMF to constant volume in a 100mL volumetric flask to obtain a concentration of 1.0×10 -3 For the probe stock solution of M, pipette the probe stock solution into a 100mL volumetric flask, and use the solvent DMF to make up the volume in the 100mL volumetric flask to obtain a concentration of 1.0×10 -4 M probe solution;

[0049] Step 2: Dissolve aluminum nitrate nonahydrate in solvent deionized water, use solvent deionized water to set the volume in a 100mL volumetric flask, and obtain a concentration of 1.0×10 -2 The aluminum ion stock solution of M; pipette the aluminum ion stock solution into a 100mL...

Embodiment 3

[0053] Add aluminum nitrate containing nine crystalline waters to deionized water to obtain an aluminum ion solution with a concentration of 100nM, dissolve the fluorescent probe synthesized in Example 1 in DMF to obtain a fluorescent probe solution with a concentration of 10 μM, and add the fluorescent probe solution to 15ml of fluorescent probe Add 10ml of aluminum ion solution to the needle solution, let it stand for 30min, the excitation wavelength is 390nm, test the fluorescence intensity, the excited state absorption spectrum and fluorescence emission spectrum of the complex of probe and aluminum ion in DMF solution. Such as figure 1 Excited with 390nm light (probe-Al 3+ ) complex, its emission peak is located at 455nm, and the ligand fluorescent probe (10μM) measures the fluorescence intensity at an excitation wavelength of 390nm.

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Abstract

The invention provides a method for detecting aluminum ions by using a reactive dye fluorescence probe. The method comprises the following steps: 1, dissolving the reactive dye fluorescence probe in a solvent, adding the solvent to a constant volume in order to obtain a probe stock solution, transferring the probe stock solution to a volumetric flask, and adding the solvent to a constant volume in order to obtain a probe solution; 2, dissolving an aluminum salt in the solvent, and adding the solvent to a constant volume in order to obtain an aluminum ion stock solution; 3, determining a relation between the fluorescence intensity and the aluminum ion concentration; and 4, taking an aluminum ion solution to be detected, adding the probe solution obtained in step 1 to the aluminum ion solution to be detected, adding the solvent to a constant volume, standing the obtained solution for a period of time, detecting the fluorescence intensity, and determining the content of aluminum ions in the aluminum ion solution to be detected.

Description

technical field [0001] The invention belongs to the field of functional reactive dyes and preparation thereof, in particular to a method for detecting aluminum ions by using reactive dye fluorescent probes. Background technique [0002] Metal ions in nature are closely related to life science, environmental science, medicine and other fields that human beings rely on for survival. Brain tissue has an affinity for aluminum, and excessive aluminum deposition in brain tissue can cause memory loss , mental retardation, especially for the elderly and children, especially for brain tissue and intellectual hazards, causing abnormal behavior, mental retardation, motor tremor, unresponsiveness, etc. However, because the early symptoms of aluminum poisoning are not obvious, they are often ignored by people, and the losses caused will be even greater. Secondly, it affects the metabolism of calcium and phosphorus, and then affects the normal function of bones, resulting in bone decalci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 光善仪张月华魏刚徐洪耀
Owner DONGHUA UNIV
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