Colloidal gold immunocolorimetry kit for detecting neutrophil gelatinase associated lipocalin (NGAL) and preparation method of kit

A colloidal gold and kit technology, applied in the field of in vitro diagnostic reagents, can solve the problems of stability, poor analytical sensitivity, high detection cost and low accuracy, and achieve the effects of good repeatability, high sensitivity and high specificity

Inactive Publication Date: 2016-08-24
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the two methods of ELISA and adsorption chromatography have many shortcomings such as poor repeatability, cumbersome operation, poor linearity, and low accuracy; while the latex immunocolorimetric method has high detection costs, poor stability, and poor analytical sensitivity. It is prone to precipitation, and the complex formed after the reaction should not be cleaned by the automatic biochemical instrument.
[0005] The purp...

Method used

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  • Colloidal gold immunocolorimetry kit for detecting neutrophil gelatinase associated lipocalin (NGAL) and preparation method of kit
  • Colloidal gold immunocolorimetry kit for detecting neutrophil gelatinase associated lipocalin (NGAL) and preparation method of kit
  • Colloidal gold immunocolorimetry kit for detecting neutrophil gelatinase associated lipocalin (NGAL) and preparation method of kit

Examples

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Embodiment 1

[0027] Preparation of NGAL calibrator

[0028] Dissolve the recombinant human NGAL protein (commercially available) in the calibrator diluent (bovine serum albumin 0.5%, sodium azide 0.1%, PBS buffer pH 8.0) to prepare calibrator with different concentration gradients, and use Jiuqiang NGAL latex to immunize The colorimetric kit was used to detect and prepare calibrator, and each concentration gradient was tested 3 times, and the average value of the test results was taken, and the fixed value was 0ng / L (calibration substance dilution, without NGAL protein added), 1.875ng / L, 165ng / mL , 613ng / mL, 1297ng / mL, 2511ng / mL, 4873ng / mL.

[0029] Preparation of Reaction Buffer Reagent (Reagent 1)

[0030] Take 80mL of 50mM PBS pH8.0 buffer and add PEG6000-200005g, polysucrose-400 1g, bovine serum albumin 0.5g, sodium azide 0.1g, and finally use the corresponding buffer to dilute to 100mL, and use 0.22um micro Filter through a pore filter to make a reaction buffer reagent (reagent 1), ...

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Abstract

The invention provides a colloidal gold immunocolorimetry kit for detecting neutrophil gelatinase associated lipocalin (NGAL) and a preparation method of the kit and belongs to the field of in-vitro diagnostic reagents. The kit is used for judging whether the situation of individual acute kidney injury occurs or not. According to the method, colloidal gold particles are adopted as a carrier, a neutrophil gelatinase associated lipocalin (NGAL for short) antibody and the colloidal gold particles are coupled, NGAL protein in a sample makes contact with the coupled gold particles in a corresponding detection device, an antigen-antibody-gold compound is formed, the light absorbance of the gold particles under detection wavelength is changed, the change amount of the light absorbance is positively correlated with the concentration of NGAL protein, and then the purpose of detecting NGAL protein in the sample is achieved. The kit has high analysis sensitivity.

Description

field of invention [0001] The invention belongs to the field of in vitro diagnostic reagents, in particular to a colloidal gold immunocolorimetric assay kit for detecting neutrophil gelatinase-associated lipid (NGAL) technical background [0002] Acute kidney injury (AKI) is a short-term clinical syndrome of decreased renal function. Patients may experience a sharp decline in renal function within hours or days due to medication, trauma, surgery, or other reasons. The main cause of the disease is renal ischemia and reperfusion injury caused by decreased renal perfusion leading to hemodynamically mediated glomerular filtration rate, but no renal parenchymal injury. If the decrease in renal perfusion can be corrected in time, the hemodynamic damage can be reversed and the renal function can be recovered rapidly in a short period of time. Otherwise, if it continues to develop, it will lead to further deterioration of the condition and develop into renal tubular necrosis (ATN)...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 郭成志王志新
Owner CHEMCLIN DIAGNOSTICS CO LTD
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