Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and kit for separating rare cells from peripheral blood

A technology for rare cells and isolated cells, applied in the field of biomedicine, can solve the problems of relying on subjective judgment, poor sensitivity and specificity, and achieve the effect of good application scenarios, no impact on repeatability, and broad application prospects.

Active Publication Date: 2016-08-24
郝淮杰
View PDF4 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods currently used for tumor diagnosis can be classified into three categories: pathology (biopsy sections), imaging (ultrasound, X-ray, CT, PET, etc.) and serology (serum tumor-associated proteins, such as CA-125, CA- 199, CEA, etc.), but these methods have inevitable shortcomings, such as poor sensitivity and specificity, and rely too much on the subjective judgment of doctors, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and kit for separating rare cells from peripheral blood
  • Method and kit for separating rare cells from peripheral blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Isolation of rare cells in peripheral blood

[0013] 1. Experimental materials

[0014] 1. Cells: breast cancer cell lines, SK-BR-3, MCF-7, ZR 75-1, MDA-MB-453; human promyelocytic leukemia cells HL-60, human Burkitt's lymphoma cells Raji, human peripheral blood Leukemia T cell Jurkat was purchased from Peking Union Medical College Cell Resource Center.

[0015] 2. Reagents and instruments: Dynabeads CD45, RPMI-1640, fetal bovine serum, RedCMXRos, Hoechst 33342, Pancreatin, HulaMixer TM The sample mixer was purchased from Life Technologies. Cell separation fluid Ficoll-Paque Plus was purchased from GE Healthcare. ACD anticoagulated blood collection tubes, 15ml and 50ml centrifuge tubes were purchased from BD Company. The magnetic frame was purchased from Promega. Cell culture flasks, petri dishes and 6-well plates were purchased from Corning.

[0016] 2. Experimental method

[0017] 1. Tumor cell markers

[0018] Breast cancer cells SK-BR-3, MCF-7, ZR 75-1, MDA-M...

Embodiment 2

[0029] Example 2 Isolation of circulating tumor cells in the peripheral blood of breast cancer patients

[0030] 1. Experimental materials

[0031] Dynabeads CD45, rabbit anti-cytokeratin (Pan) polyclonal antibody, and goat anti-rabbit IgG labeled with Alexa Fluor 594 were purchased from Life Technologi es. 50ml and 15ml centrifuge tubes were purchased from Corning Company. Ficoll-Paque Plus was purchased from GE Healthcare. BD Vacutaiher blood collection tube (sodium citrate anticoagulant) was purchased from Becton-Dickinson. Mounting tablets containing DAPI were purchased from VictorLabs.

[0032] 2. Experimental method

[0033] Use a vacuum blood collection device to collect peripheral blood from healthy volunteers or breast cancer patients, discard the first 2ml of blood and collect 1ml of blood into the ACD anticoagulated blood collection tube. Transfer the above-mentioned peripheral blood to a 50ml cell centrifuge tube, add 9ml PBS and mix well. Take 0.125ml Dynabeads CD45,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for separating rare cells from peripheral blood and a kit using the same. The method integrates a negative enrichment technology and a density gradient centrifugation technology, has the advantages of high recovery rate, simpleness, and convenience, and generates little damage to rare cells in peripheral blood. By using the provided kit, tumor cells such as breast cancer cells can be separated from peripheral blood through the separation method. The kit and method can also be applied to early diagnosis, relapse monitoring, and drug effect evaluation, can be used to separate rare cells in body liquids such as pleural fluid, ascitic fluid, and the like, and have a wide application prospect.

Description

Technical field [0001] The present invention relates to the field of biomedicine, in particular to a method for separating rare cells in peripheral blood, and also to a kit using the method. Background technique [0002] Due to environmental pollution and people maintaining a variety of bad habits, such as smoking and eating unhealthy foods, the number of cancer patients worldwide is increasing at an alarming rate. In 2012, there were an estimated 14 million new cancer cases worldwide. The International Agency for Research on Cancer under the World Health Organization recently published the 2014 World Cancer Report, stating that this number is expected to rise to 22 million within 20 years. "Early prevention, early detection, and early treatment" is currently the best way to prevent cancer. The methods currently used for tumor diagnosis can be classified into three categories: pathology (biopsy section), imaging (ultrasound, X-ray, CT, PET, etc.) and serology (serum tumor-relate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/48
Inventor 郝淮杰
Owner 郝淮杰
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com