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Rhodobacter sphaeroides K73 bacterial strain and application of rhodobacter sphaeroides K73 bacterial strain to surface water ecological purification

A technology of Rhodobacter sphaeroides and strains, applied in the field of Rhodobacter sphaeroides K73 strain and its ecological purification of surface water, can solve the problems of difficulty in effectively reducing organic pollution in water, low removal rate of dissolved organic matter, side effects, etc., and achieve good results Water purification effect, ecological purification effect is obvious, and the effect of purification cost is low

Inactive Publication Date: 2016-08-17
李可俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods (filtration, adsorption and flocculation) are difficult to maintain a relatively good purification effect in a slightly longer period of time, and the technical costs are often relatively high, and there are some side effects
In addition, for some water bodies rich in nitrogen and phosphorus, it is very easy to cause explosive growth of algae due to eutrophication of the water body. Although conventional physical and chemical methods can have a good removal effect on suspended organic matter in the water body, but for The removal rate of dissolved organic matter is very low, and it is difficult to effectively reduce organic matter pollution in water

Method used

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  • Rhodobacter sphaeroides K73 bacterial strain and application of rhodobacter sphaeroides K73 bacterial strain to surface water ecological purification
  • Rhodobacter sphaeroides K73 bacterial strain and application of rhodobacter sphaeroides K73 bacterial strain to surface water ecological purification
  • Rhodobacter sphaeroides K73 bacterial strain and application of rhodobacter sphaeroides K73 bacterial strain to surface water ecological purification

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Experimental program
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Effect test

Embodiment 1

[0051] The present embodiment provides a kind of microbial bacterial agent containing water purification bacterial strain and preparation method thereof, and the method comprises the following steps:

[0052] S11: adding the strain of Rhodobacter sphaeroides into a small amount of LB liquid medium and culturing at 28° C. for 60 hours to obtain a bacterial liquid. The obtained bacterial liquid was inoculated in LB liquid medium at a volume ratio of 5% and cultivated at 28°C for 60 hours to obtain an amplified culture liquid;

[0053] S12: Filter the amplified culture solution with a 400-mesh filter, and wash the filtered bacteria into an appropriate amount of LB liquid medium to obtain a liquid bacterial agent.

[0054] Wherein, the LB liquid medium mentioned above contains peptone 10g / L, yeast powder 5g / L, sodium chloride 5-10g / L, pH 7.0, and all of them are sterilized before use.

Embodiment 2

[0056] The present embodiment provides microbial bacterial agent containing water body purification bacterial strain and preparation method thereof, and the method comprises the following steps:

[0057] S21: Isolation and purification of Rhodobacter sphaeroides;

[0058] Specifically, in this step, the river bottom silt of Xiaoqing River in Beijing is used as a sample, and the sample taken is subjected to gradient dilution and then plated for culture, wherein the medium used is a solid LB medium; finally, the culture medium obtained is collected. A single colony was obtained to obtain Rhodobacter sphaeroides.

[0059] For the collected Rhodobacter sphaeroides, its 16srRNA was amplified by conventional methods and sequenced, and its sequence is shown in SEQ ID NO:1.

[0060] S22: the isolated and purified Rhodobacter sphaeroides is prepared into a bacterial liquid;

[0061] In the step S22, a small amount of the isolated and purified single colony in the step S21 is put into...

Embodiment 3

[0067] The present embodiment provides a kind of microbial bacterial agent containing water purification bacterial strain and preparation method thereof, and the method comprises the following steps:

[0068] S31: making the isolated and purified Rhodobacter sphaeroides into a bacterial liquid;

[0069] In step S31, the single colony used is provided in step S21 in Example 2;

[0070] Introduce the single bacterium colony isolated and purified in step S21 into a small amount of LB liquid medium and shake the flask at 28-30°C for 60 hours to obtain the bacterial solution.

[0071] S32: Inoculate the bacterial solution containing Rhodobacter sphaeroides in LB liquid medium at a volume ratio of 6% and culture at 32° C. for 65 hours to obtain a culture solution;

[0072] S33: Filter the culture solution with a 400-mesh filter, and wash the filtered bacteria into LB liquid medium to obtain a microbial bacterial agent.

[0073] Wherein, the LB liquid medium mentioned above contain...

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Abstract

The invention relates to the field of microorganism application, in particular to a bacterial strain capable of being used for surface water ecological purification, a bacterium agent with the same and an application. The bacterial strain is rhodobacter sphaeroides K73, and is collected in the China general microbiological culture collection Center, the collection number is CGMCC NO.12310, and the collection time is March 28, 2016. By means of the bacterial strain, the nitrogen content, the phosphorus content and the like in water can be effectively reduced, the water transparency can be obviously increased, and the technical effect of improving water quality is achieved. Compared with the traditional ecological water purification technology based on animals and plants, use and the management modes of the bacterial strain are simpler; compared with the traditional water-quality purification technology based on a microorganism bacterium agent, the ecological purification effect of the bacterial strain is more obvious; compared with the traditional treatment means in the physical or chemical aspect, the bacterial strain is free of ecological side effect and low in purification cost.

Description

technical field [0001] The invention relates to the field of microbial application, in particular to a strain of Rhodobacter sphaeroides K73 and its application in ecological purification of surface water bodies. Background technique [0002] In areas where sewage interception and drainage and rain and sewage diversion have been completed, surface water bodies are often in a state of yellowing, greening or even blackening and smelling due to atmospheric dustfall and non-point source pollution. [0003] In order to maintain a good water environment, various water treatment technologies are being developed and used. Among them, artificially constructing a water ecosystem with aquatic animals and plants as the main body to purify water quality is a relatively popular form and has been widely used. However, the ecological purification methods based on water animals and plants have the advantages of low cost and environmental protection, but maintenance and management are more tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/01Y02W10/37
Inventor 李可俊
Owner 李可俊
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