Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of respiratory syncytial virus antigen and rapid respiratory syncytial virus antibody detection kit prepared from antigen

A technology of syncytial virus and respiratory tract, applied in the field of clinical medical testing, can solve the problem of low yield and achieve the effect of high specificity and sensitivity

Inactive Publication Date: 2016-08-17
LANZHOU YAHUA BIOTECH
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sources of F protein currently used are mainly directly purified from viruses or expressed in eukaryotic systems, and there are problems such as low yields

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of respiratory syncytial virus antigen and rapid respiratory syncytial virus antibody detection kit prepared from antigen
  • Preparation method of respiratory syncytial virus antigen and rapid respiratory syncytial virus antibody detection kit prepared from antigen
  • Preparation method of respiratory syncytial virus antigen and rapid respiratory syncytial virus antibody detection kit prepared from antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] 1) Recombinant expression, structural renaturation and purification of recombinant respiratory syncytial virus F protein antigen: Refer to GenBank sequence U63644.1 for the gene of respiratory syncytial virus F protein, select part of the gene for synthesis (AA162-387aa), remove the signal peptide sequence and Escherichia coli rare codon optimization was carried out, the expression vector pET30a, the restriction site of ligation was BamH I / Hind III, the total target gene was 681bp (226aa), transformed into BL21 (DE3), and the expressed recombinant protein was 274aa in total, with a molecular weight of 30.8 kDa , the isoelectric point is 7.66, and the recombinant protein is expressed in the form of inclusion body.

[0035] Construction and identification of recombinant expression vectors: Respectively use BamH I and Hind III to double-enzyme digest the target gene fragment F1 and pET30a of respiratory syncytial virus, purify and recover the digested products, connect over...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention relates to a respiratory syncytial virus antigen for diagnosing respiratory syncytial virus infection, a method for preparing the antigen, a method and a reagent for detecting respiratory syncytial virus infection by using the antigen, and belongs to the field of clinical medical detection. The artificially synthesized optimized respiratory syncytial virus F1 gene was constructed as a prokaryotic expression vector, and Escherichia coli was used to express the respiratory syncytial virus F1 antigen, and inclusion bodies with three-dimensional structure and immunity were obtained by renaturation by dialysis, gradient dilution and gel chromatography. Active recombinant respiratory syncytial virus F1 protein antigen. The present invention applies colloidal gold immunochromatography technology, uses engineered expressed respiratory syncytial virus F1 antigen as detection antigen, uses high-efficiency anti-human IgG or IgM monoclonal antibody as capture antibody, and filters red blood cells with blood filter membrane, according to "indirect method" The principle is to prepare a rapid detection kit for respiratory syncytial virus antibody, which is used to detect respiratory syncytial virus antibody in human serum, plasma or whole blood. It has the advantages of simple detection method, accurate and rapid result display, and no need for special equipment.

Description

technical field [0001] The invention relates to a respiratory syncytial virus antigen for diagnosing respiratory syncytial virus infection, a method for preparing the antigen, a method and a reagent for detecting the respiratory syncytial virus with the antigen, and belongs to the field of clinical medical detection. The invention has important significance in the diagnosis of infant acute lower respiratory tract infection caused by respiratory syncytial virus. Background technique [0002] Respiratory syncytial virus (RSV), referred to as syncytial virus, is the most important viral pathogen of acute lower respiratory tract infection in infants and young children around the world. It is widespread and has a high incidence. About 50% of infant pneumonia and 90% of infant Bronchitis is caused by RSV infection. In developing countries, the hospitalized mortality rate of children under 5 years old due to RSV infection is 7%, while in developed countries it is 0.5%~2.0%. About...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/135G01N33/569
CPCC07K14/005C12N2760/18522G01N33/56983
Inventor 李克生杜惠芬
Owner LANZHOU YAHUA BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com