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Oil-phase composition for generating water-in-oil liquid drops with centrifugation method

A composition, water-in-oil technology, which is used in laboratory containers, microorganism determination/inspection, transportation and packaging, etc., can solve the problem that the thermal stability of droplets cannot reach PCR reaction temperature conditions, etc., and achieve a convenient method. , low cost, good stability effect

Active Publication Date: 2016-08-17
因纽美瑞克斯公司
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Problems solved by technology

However, in this experiment, it was found that although this type of formulation can be used as a continuous phase in the microfluidic chip to produce water droplets of uniform size with almost no damage, it can also produce uniform and stable droplets by centrifugation, but the generated droplet heat The stability cannot reach the temperature conditions of the PCR reaction

Method used

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  • Oil-phase composition for generating water-in-oil liquid drops with centrifugation method
  • Oil-phase composition for generating water-in-oil liquid drops with centrifugation method
  • Oil-phase composition for generating water-in-oil liquid drops with centrifugation method

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Embodiment Construction

[0019] The first liquid is PCR reaction liquid, its composition is: 1×PCR buffer, 5mM MgCl 2 , 0.4 mM dNTPs, 1% Platinum Taq Polymerase. On the basis of using the combination of carbonic acid diester, mineral oil and surfactant as the oil phase composition in the prior art to adjust the amount of each component, in order to find the one that meets the requirements of density and thermal stability, it can react with PCR by centrifugation The ratio of the oil phase composition of the liquid to obtain a stable emulsion system. The component contents of Examples 1-34 are shown in Table 1.

[0020] The oil phase composition formula (v / v) of table 1 embodiment 1-34

[0021]

[0022]

[0023] Use the generating device of the droplet in CN 104741158 A (such as figure 1 Shown), wherein reference numeral 1 is a droplet tube, 2 is a collecting tube, 3 is a first liquid, and 4 is a second liquid. The PCR reaction solution is used as the first liquid, and the oil phase mixture in...

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Abstract

The invention provides an oil-phase composition for generating water-in-oil liquid drops with a centrifugation method. The oil-phase composition comprises 7%-15% of silica-chain nonionic surfactant containing long-chain alkyl, 0%-10% of mineral oil and the balance diethylhexyl carbonate, or comprises 85%-95% of long-chain alkane ester and 5%-15% of silica-chain nonionic surfactant containing long-chain alkyl, or comprises silicone oil and surfactant. The invention further provides a method for generating the water-in-oil liquid drops with the centrifugation method with the oil-phase composition as second liquid.

Description

technical field [0001] The present invention relates to an oil phase composition for producing liquid droplets, and a method for producing water-in-oil droplets by centrifugation using the oil phase composition as a second liquid. Background technique [0002] Microfluidics is a widely applicable method to achieve dropletization, but it has limitations, such as the inability to achieve high throughput, the preparation and use of microfluidic chips requires high environmental cleanliness, and cumbersome operations. Therefore, we propose a centrifugation method to generate liquid droplets (see CN104741158A). In the centrifugation method, the density of the oil phase is required to be lower than that of water so that the water phase droplets produced by the dropletization device sink quickly, thereby reducing the collision between the droplets, and the droplets must have good thermal stability (such as PCR). The reaction needs to be 95°C) and biocompatibility to ensure the occ...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12N15/10B01F17/54C09K23/00C09K23/54
CPCB01L3/5027C12N15/10C09K23/017C09K23/54C12Q2531/113B01L3/00C12Q1/6806C09K23/018
Inventor 黄岩谊陈子天张芳丽廖珮宇
Owner 因纽美瑞克斯公司
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