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Stem cell induced culture solution used for eyes and preparation method and application thereof

A cell culture and culture medium technology, applied in cell culture active agents, biochemical equipment and methods, animal cells, etc., and can solve problems such as failure, donor rejection surgery, and limited number of donors

Active Publication Date: 2016-08-10
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Corneal donors generally come from corneal donors. On the one hand, the number of donors is limited. On the other hand, there is still an immune reaction after allogeneic corneal transplantation, and even donor rejection occurs, resulting in surgical failure.

Method used

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  • Stem cell induced culture solution used for eyes and preparation method and application thereof
  • Stem cell induced culture solution used for eyes and preparation method and application thereof
  • Stem cell induced culture solution used for eyes and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In this example, human orbital adipose tissue was used to isolate and culture ASCs in vitro to obtain the cell culture medium of the present invention.

[0043] Then, the rabbit corneal chemical burn model was made with sodium hydroxide (NaOH) solution, and the rabbit corneal chemical injury was treated with conditioned culture solution as eye drops, and the therapeutic effect on corneal inflammation, edema and ulcer was observed.

[0044] 1 Materials and methods

[0045] 1.1 Main instruments and reagents

[0046]Fetal bovine serum (FBS, Gibco, U.S.), low-sugar DMEM medium (HyClone, U.S.), type I collagenase (Worthington, U.S.), rabbit anti-vascular endothelial growth factor (VEGF, AbCam, U.S.), Sumianxin II injection (Dunhua Shengda Animal Pharmaceutical Co., Ltd., Jilin), proparacaine hydrochloride eye drops (Alcon, Belgium), fluorescein sodium detection test paper (Tianjin Jingming New Technology Development Co., Ltd.), 0.22 μm filter ( Merck Millipore, Ireland), s...

Embodiment 2

[0058] In this example, sodium hydroxide (NaOH) solution was used to create a rabbit corneal chemical burn model. The culture solution of Example 1 was used as eye drops to treat rabbit corneal chemical injury, and the therapeutic effect on corneal inflammation, edema and ulcer was observed.

[0059] 1 Preparation of rabbit corneal alkali burn model

[0060] 1.1 Experimental animal health Purebred New Zealand white rabbits were provided by the Animal Experiment Center of Shanghai Tenth People's Hospital, 4 months old, 6 in total, male or female, weighing 2.0-2.5kg, without eye diseases.

[0061] 1.2 Making the Rabbit Corneal Alkaline Burn Model

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PUM

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Abstract

The invention belongs to the field of biotechnology and provides a stem cell induced culture solution used for eyes .The stem cell induced culture solution comprises a vascular endothelial cell growth factor (VEGF), a basic fibroblast growth factor (bFGF) and an epidermal growth factor (EGF); the content of the vascular endothelial cell growth factor (VEGF) is 0.5-2.5 ng / ml, the content of the basic fibroblast growth factor (bFGF) is 0.25-0.4 ng / ml, and the content of the epidermal growth factor (EGF) is 0.5-2.0 ng / ml .The stem cell (ASCs) in vitro conditioned culture medium from human eyepit fat is adopted, and a significant reinforcement function is achieved for regeneration and repair of corneal injury.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a stem cell induction culture medium for eyes, its preparation method and its application. Background technique [0002] Corneal trauma is a common disease in ophthalmology clinics, and it can even lead to blindness in severe cases. The normal corneal epithelialization process is completed by the regeneration and differentiation of stem cells in the basal layer of the limbal epithelium. Corneal injury leads to corneal epithelial defect, and the secondary inflammatory reaction will affect the proliferation and migration of stem cells, form pannus, aggravate corneal edema and scarring, corneal turbidity affects light transmission, and even cause corneal ulcers. Corneal transplantation is still the only reliable and effective treatment for clinical corneal blindness. Corneal donors generally come from corneal donors. On the one hand, the number of donors is limi...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K35/28A61P27/02
CPCA61K35/28C12N5/0667C12N2501/11C12N2501/115C12N2501/165
Inventor 刘广鹏廖彩荷许益聘谭健韩芳叶信海
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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