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Kit for detecting residual thiophosphate organophosphorus pesticides and application method of kit

A technology of phosphorothioate and organophosphorus pesticides, which can be used in measuring devices, analyzing materials by chemical reactions, and analyzing materials by observing the influence of chemical indicators, etc. It can solve the problem of poor storage stability of nano-gold problems, to achieve the effect of improving storage stability, improving stability, and improving detection stability

Active Publication Date: 2016-08-03
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (2) The storage stability of nano gold is poor
These all limit the application of nano-gold colorimetric method in the detection of organophosphorus pesticide residues to a certain extent.

Method used

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  • Kit for detecting residual thiophosphate organophosphorus pesticides and application method of kit
  • Kit for detecting residual thiophosphate organophosphorus pesticides and application method of kit
  • Kit for detecting residual thiophosphate organophosphorus pesticides and application method of kit

Examples

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Effect test

Embodiment 1

[0044] A detection kit for organophosphorus pesticide residues, comprising an extractant, a reducing agent, a precursor solution, a pH regulator, a 1mL dropper and a 5mL reaction tube. Wherein, the extractant is 5-10mL of acetone: water mixed with acetone solution at a volume ratio of 1:1; The body fluid is 0.8-4mM chloroauric acid aqueous solution; the pH regulator is 3.875-7.75M sodium hydroxide aqueous solution.

[0045] Wherein, the preparation method of tyrosine methyl ester grafted hyaluronic acid is: 1. first in the reaction vessel, add 4.2 parts of ultrapure water, add 0.041 part of 2-(N-morpholine) sodium ethanesulfonate (MES), After fully dissolving, an aqueous solution is obtained;

[0046] ②Add 1.2 parts of hyaluronic acid (molecular weight 66kDa-99kDa) and 6.8 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) to the aqueous solution obtained in step ① under stirring With 1.5 parts of N-hydroxysuccinimide (NHS), react for 130min to obtain a...

Embodiment 2

[0058] A detection kit for organophosphorus pesticide residues, comprising an extractant, a reducing agent, a precursor solution, a pH regulator, a 1mL dropper and a 5mL reaction tube. Wherein, the extractant is 5-10mL of acetone: water mixed with acetone solution at a volume ratio of 1:1; The body fluid is 0.8-4mM chloroauric acid aqueous solution; the pH regulator is 3.875-7.75M sodium hydroxide aqueous solution.

[0059] Wherein, the preparation method of tyrosine methyl ester grafted hyaluronic acid is:

[0060] ①In the reaction vessel, add 4.2 parts of ultrapure water, add 0.041 parts of 2-(N-morpholine) sodium ethanesulfonate (MES), and fully dissolve to obtain an aqueous solution;

[0061] ②Add 0.8 parts of sodium hyaluronate (molecular weight 66kDa-99kDa) and 4.2 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) to the aqueous solution obtained in step ① under stirring. 1 part and 1 part of N-hydroxysuccinimide (NHS), reacted for 130min to obta...

Embodiment 3

[0072] A detection kit for organophosphorus pesticide residues, comprising an extractant, a reducing agent, a precursor solution, a pH regulator, a 1mL dropper and a 5mL reaction tube. Wherein, the extractant is 5-10mL of acetone: water mixed with acetone solution at a volume ratio of 1:1; The body fluid is 0.8-4mM chloroauric acid aqueous solution; the pH regulator is 3.875-7.75M sodium hydroxide aqueous solution.

[0073] Wherein, the preparation method of tyrosine methyl ester grafted hyaluronic acid is:

[0074] ①In the reaction vessel, add 4.2 parts of ultrapure water, add 0.041 parts of 2-(N-morpholine) sodium ethanesulfonate (MES), and fully dissolve to obtain an aqueous solution;

[0075] ②Add 1.6 parts of sodium hyaluronate (molecular weight 66kDa-99kDa) and 8.4 parts of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) to the aqueous solution obtained in step ① under stirring. part and 2 parts of N-hydroxysuccinimide (NHS), reacted for 130min to obtai...

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Abstract

The invention relates to the field of residual pesticide detection, in particular to a kit for detecting residual thiophosphate organophosphorus pesticides and an application method of the kit.The kit comprises an extracting agent, a reducing agent, precursor liquid, a pH controlling agent, a dropper and a reaction tube; the reducing agent is a tyrosine methyl ester grafted hyaluronic acid aqueous solution of 2.4 ng / mL to 24 micrograms / mL.According to the kit for detecting the residual thiophosphate organophosphorus pesticides and the application method of the kit, the organophosphorus pesticides are added in the gold nanoparticle formation process, the three processes of gold nanoparticle formation and aggregation and protection layer establishment are synchronized, the aggregation degree of gold nanoparticles is controlled in the formation process of the gold nanoparticles, the surfaces of formed gold nanoparticle aggregates are provided with protection layers, the stability of the aggregates is greatly improved, the detection stability is improved, the detection result is not limited by the detection time any more, and repetition is easy.

Description

technical field [0001] The invention relates to the field of detecting pesticide residues, in particular to a detection kit for phosphorothioate organic phosphorus pesticide residues and an application method thereof. Background technique [0002] Organophosphorus pesticides have become one of the three pillars of pesticides in the world because of their broad-spectrum, high efficiency, and low price. Among them, phosphorothioate is a commonly used class of organophosphorus pesticides. Dimethoate, phorate, malathion and other pesticides with strong toxicity and good insecticidal effect belong to this category. important role in high yield. However, because users lack the knowledge of scientific use of pesticides and one-sided pursuit of production, the agricultural and sideline products circulating in the market have pesticide residues to varying degrees, which has become a hidden danger that threatens people's lives and health. In order to ensure "safety on the tip of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 杨丽敏姜磊张晓慧李慧萍李硕
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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