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Method for detecting Acanthopanax senticosus and Glycyrrhiza uralensis preparation and application thereof

A technology of Acanthopanax senticosus licorice and detection method, which can be applied to measurement devices, instruments, scientific instruments and other directions, and can solve problems such as unfavorable quality and difficulty in detection work.

Inactive Publication Date: 2016-07-27
LUOYANG HUIZHONG ANIMAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is currently no detection method for Acanthopanax licorice preparations, which is not conducive to controlling its quality. In order to ensure safe, effective and stable clinical use
In particular, it is difficult to extract syringin and other substances in the Acanthopanax licorice preparation, which also caused great difficulties in the detection work

Method used

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  • Method for detecting Acanthopanax senticosus and Glycyrrhiza uralensis preparation and application thereof
  • Method for detecting Acanthopanax senticosus and Glycyrrhiza uralensis preparation and application thereof
  • Method for detecting Acanthopanax senticosus and Glycyrrhiza uralensis preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 prepares Acanthopanax licorice preparation

[0060] Soak Acanthopanax and Radix Glycyrrhiza in two flavors of Chinese herbal medicines (both purchased from Luoyang Kangxin Chinese Herbal Pieces Co., Ltd., Acanthopanax batch number 20120315, and Licorice root batch number 20120428), add 8 times the amount of water to soak for 1.5 hours, and reflux under reduced pressure at 80°C Extract for 2 hours, filter, extract the filter residue with 8 times the amount of water once, combine the filtrates, centrifuge while hot, concentrate the supernatant under reduced pressure to a relative density of about 1.1 (≤70°C), and spray dry to obtain 250 g of the finished product.

Embodiment 2

[0061] Example 2 Study on the specificity of the identification method of Acanthopanax licorice preparation

[0062] (1) Reagents and samples

[0063] Positive control substance: isoxapidine, source: China Institute for the Control of Pharmaceutical and Biological Products, content: 99.8%, batch number: 110837-200304.

[0064] Sample: Acanthopanax licorice powder, prepared according to Example 1.

[0065] Negative reference substance: Negative reference substance for each identification item was prepared according to the method in Example 1.

[0066] (2) Identification of Acanthopanax

[0067] Take 2.5 g of the sample prepared in Example 1, add 50 ml of methanol, heat and reflux for 1 hour, filter, evaporate the filtrate to dryness, add 10 ml of water to the residue to dissolve, shake and extract twice with chloroform, 10 ml each time, combine the chloroform solution, evaporated to dryness, and the residue was dissolved in 1ml of methanol as the test solution. Take the lic...

Embodiment 3

[0072] The research of embodiment 3 syringin content assay method

[0073] (1) Instrument:

[0074] High performance liquid chromatography, type e2695, Waters Corporation, USA

[0075] UV detector, type 2489, Waters Corporation, USA

[0076] Electronic Analytical Balance, Model AB135-S, Mettler Toledo

[0077] Reagent:

[0078] Acetonitrile Chengdu Kelong Chemical Reagent Factory, chromatographically pure, 20130221

[0079] homemade purified water

[0080] Control substance:

[0081] Syringin, source: China Institute for the Control of Pharmaceutical and Biological Products, content: 100%, batch number: 111574-200502.

[0082] sample:

[0083] Prepared according to Example 1.

[0084] (2) Chromatographic conditions

[0085] The chromatographic column is an Agilent Eclipse XDBC18 column (250×4.6mm, 5 μm); acetonitrile is used as the mobile phase A; water is used as the mobile phase B, and the gradient elution is carried out according to the regulations in Table 3; the de...

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Abstract

The invention relates to a method for detecting an Acanthopanax senticosus and Glycyrrhiza uralensis preparation. The Acanthopanax senticosus and Glycyrrhiza uralensis preparation comprises Acanthopanax senticosus and Glycyrrhiza uralensis. The method is characterized in that the method comprises a qualitative detection of the Acanthopanax senticosus and Glycyrrhiza uralensis preparation and a quantitative detection of the Acanthopanax senticosus and Glycyrrhiza uralensis preparation, wherein the qualitative detection is carried out for respective identification of Acanthopanax senticosus and Glycyrrhiza uralensis by thin layer chromatography, and the quantitative detection is carried out for respective detection of contents of syringing and glycyrrhizic acid by high performance liquid chromatography. The invention also relates to an application of the method.

Description

technical field [0001] The invention relates to a detection method of a preparation, in particular to a detection method of a pharmaceutical preparation. The invention also relates to the application of the detection method. Background technique [0002] In recent years, Chinese herbal preparations have been widely used in animal breeding in my country, and have achieved good comprehensive effects in accelerating growth, improving production performance, enhancing body immunity, anti-virus, and anti-stress, and have no drug resistance, No residue, small side effects, significant effect and so on. [0003] Acanthopanax and licorice are important traditional Chinese medicine raw materials. [0004] Among them, Acanthopanax (Latin name is Acanthopanax senticosus) is Araliaceae Aralis, a deciduous shrub whose roots and rhizomes can be used as medicine. It contains a variety of active chemical components, and the important components isolated at present are glycosides. Eleuthe...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/90
Inventor 张许科刘兴金张继颖
Owner LUOYANG HUIZHONG ANIMAL MEDICINE
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