A strain antagonizing Fusarium moniliforme of heterophylla heterophylla and its application

A technology of Fusarium moniliformes and Radix Radix is ​​applied in the directions of application, bacteria, fungicides, etc., which can solve the problems of environmental pollution, increase production cost, and degrade the function of farmland ecosystem, and achieve strong antagonistic effect and good effect.

Active Publication Date: 2019-04-02
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, there are serious continuous cropping obstacles in the cultivation process of Pseudostellaria Radix Pseudostellariae (such as figure 1 As shown), the growth and development of Pseudostellariae Pseudostellariae in continuous cropping is poor, the underground part cannot expand normally, and soil-borne diseases are serious. Usually, the same plot can be replanted after 2 to 4 years after harvesting, which seriously restricts the sustainable production and utilization of Pseudostellariae Pseudostellaria
The problem of continuous cropping obstacles has also led to a series of downstream unfavorable problems. For example, the production area and scale of Taizisandao are shrinking year by year, and even the production area has moved out, and authenticity is distorted.
At the same time, when the causes of continuous cropping obstacles and the mechanism of action are not yet clear, most drug farmers try to maintain the yield of heterophylla by increasing fertilizers and abusing pesticides, but the results are often not good, which increases production costs and leads to environmental pollution, Pesticide residues in traditional Chinese medicinal materials exceed standards and farmland ecosystem function degradation has caused the production of heterophylla to fall into a vicious circle

Method used

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  • A strain antagonizing Fusarium moniliforme of heterophylla heterophylla and its application
  • A strain antagonizing Fusarium moniliforme of heterophylla heterophylla and its application
  • A strain antagonizing Fusarium moniliforme of heterophylla heterophylla and its application

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1 antagonistic antibacterial Pseudomonas ( Pseudomonas protegens LK-313) screening and identification

[0022] 1. Screening of antagonistic bacteria of Pseudostellaria heterophylla specialized Fusarium moniliforme

[0023] Antagonistic antibacterial Pseudomonas of the present invention ( Pseudomonas protegens LK-313) was screened from the rhizosphere soil of Pseudostellaria heterophylla.

[0024] 1.1 Preparation of Pseudomonas culture medium

[0025] Pseudomonas selective isolation agar (PSIA) preparation method is as follows: Weigh 20 g of soybean-casein digest agar (soybean casein digest agar, SCD) (BD, USA), add 495.5 mL Heat and stir in double-distilled water, after fully dissolving, add 1 mL of 0.1% (wt / vol) crystal violet stock solution, sterilize at 121°C for 15 minutes at high temperature and high pressure, cool to about 50°C, add 3.5 mL to the culture medium 5% (wt / vol) furanidine stock solution, mixed well, poured quickly, cooled and solidifie...

Embodiment 2

[0037] Example 2 Bacteriostatic effect detection of antagonistic bacteria

[0038] Using PDA medium, inoculate activated Pseudomonas LK-313 at a distance of 2.5 cm from the center of the petri dish, place it in a constant temperature incubator at 28°C for 48 hours, and inoculate Pseudomonas pseudomonas at the center of the dish. Specialized Fusarium moniliforme was placed in a constant temperature incubator at 28°C in the dark for several days, and the formation of the inhibition zone was observed in real time. After 4 days of cultivation, it was found that Pseudomonas protegens LK-313 can efficiently antagonize the mycelial growth of Fusarium moniliforme in heterophylla (such as image 3 shown).

Embodiment 3

[0039] Example 3 Pseudomonas ( Pseudomonas protegens LK-313) Optimum medium optimization

[0040] Prepare the following medium to optimize the optimal growth medium of Pseudomonas LK-313, the medium is as follows: 1) whole LB solution; 2) 1 / 4 LB solution; 3) 1 / 4LB+1 / 20MS; 4 ) 1 / 4LB+1 / 40MS; 5) 1 / 4LB+1 / 80MS; 6) 1 / 4LB+1 / 20MS+0.1wt.% brown sugar; 7) 1 / 4LB+1 / 40MS+0.1wt.% brown sugar ;8) 1 / 4LB+1 / 80MS+0.1wt.% brown sugar.

[0041] Among them, the formula of MS medium (both without iron salt) is: 1) macroelements: 1900 mg / L KNO 3 , 1650 mg / LNH 4 NO 3 , 370 mg / L MgSO 4 •7H 2 O, 170 mg / L KH 2 PO 4 , 440 mg / L CaCl 2 • 2H 2 O; 2) Trace elements: 22.3mg / L MnSO 4 • 4H 2 O, 8.6 mg / L ZnSO 4 •7H 2 O, 6.2 mg / L H 3 BO 3 , 0.83 mg / L KI, 0.25 mg / LNa 2 MoO 4 •7H 2 O, 0.025 mg / L CuSO 4 • 5H 2 O, 0.025 mg / L CoCl, 2.6 mg / L H 2 O; 3) Organic matter: 2.0 mg / L glycine, 0.5 mg / L pyridoxine hydrochloride, 0.1 mg / L ammonium sulfate hydrochloride, 0.5 mg / L niacin, 100 mg / L creatine; LB...

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Abstract

The invention provides an antagonistic bacterium against Fusarium moniliforme heterophylla and its application. The antagonistic bacterium has been identified as Pseudomonas LK‑313, and its preservation number is CGMCC NO.12121. The antagonistic bacteria provided by the present invention are screened and obtained from the rhizosphere soil of Radix Pseudostellariae by Pseudomonas selective medium, plate confrontation culture shows that the antagonistic bacteria can effectively inhibit the growth of mycelia of pathogenic bacteria such as Fusarium moniliforme of Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix or Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix Radix by the antagonistic bacteria of the present invention. It has good colonization ability and no pathogenicity to other crops. Simultaneously, the present invention provides the optimum culture medium for the growth of the antagonistic bacteria. The optimal formula is 1 / 4 LB+1 / 20 MS (without iron salt)+0.1wt.% brown sugar, and the fermented bacteria liquid has obvious antagonistic effect. The invention provides antagonistic bacteria strains and bacterial agents for overcoming or alleviating the replanting diseases of the medicinal plant Pseudostellariae Pseudostellariae, which has good biocontrol effect, no pollution, safe ecology, and broad development and application prospects.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to an antagonistic bacterium which has an inhibitory effect on Fusarium moniliforme heterophylla, which can be used to overcome or alleviate the problem of soil-borne diseases in continuous cropping heterophylla, and belongs to the technical field of microorganisms and biological control. Background technique [0002] Pseudostellaria ( Pseudostellaria heterophylla ) alias children's ginseng, baby ginseng, rice ginseng, is a perennial herbaceous plant of Caryophyllaceae, and its roots are used as medicine. Included in the "List of Chinese herbal medicines that can be used in health food". Pseudostellaria is mainly produced in Fujian, Guizhou, Anhui and other places, among which Zherong County in Fujian Province has the longest cultivation history and the best quality, and is known as the "Hometown of Chinese Pseudostellaria". However, there are serious continuous cropping obstacles in the cult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
CPCA01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 吴林坤陈军林文雄吴红淼林生
Owner FUJIAN AGRI & FORESTRY UNIV
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