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Preparation method of blueberry wine fermenting yeast solid preparation

A technology of blueberry fruit wine and fermenting yeast, which is applied in the preparation of alcoholic beverages, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of not fully highlighting the characteristic flavor of the product, affecting the taste and aroma of blueberry fruit wine, and the variety and style of fruit wine Single and other problems, to achieve the effect of shortening the fermentation time, easy operation and low cost

Inactive Publication Date: 2016-07-20
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the brewing and actual production of blueberry fruit wine, there is no special yeast for blueberry fruit wine in my country at present, and most of them rely on wine special yeast, which results in relatively single variety and style of brewed fruit wine, serious homogeneity and lack of typicality
In addition, active dry yeast or wine-specific yeast does not match well with blueberries in the place of origin, which seriously affects the taste and aroma of blueberry fruit wine, cannot fully highlight the characteristic flavor of the product, and makes the product lack regional characteristics
This has hindered the development of blueberry fruit wine to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation method of blueberry fruit wine fermentation yeast solid preparation, comprises the steps:

[0021] 1) Strain activation: the yeast strain for blueberry fruit wine is used as the starting strain, and the culture medium is YEPD medium, and the strain is activated by slant passaging, the culture temperature is 26°C, and the culture time is 24h; the formula of the YEPD medium is: Yeast extract 10g, peptone 20g, glucose 20g, agar powder 18g, water 1000ml, pH 5.0, autoclaved at 121°C for 20min;

[0022] 2) Shake flask expansion culture: pick the activated strains from ring 2 and inoculate them into 50ml YEPD liquid medium, and culture them in shake flasks at 27°C and 180rpm for 20 hours to obtain the first-grade seed bacteria liquid; then, the first-grade seed bacteria solution was inoculated in 1LYEPD liquid medium at a mass ratio of 5%, and cultured again under the same conditions to obtain a secondary seed bacterial solution;

[0023] 3) The ...

Embodiment 2

[0025] Embodiment 2: the preparation method of blueberry fruit wine fermentation yeast solid preparation, comprises the steps:

[0026] 1) The blueberry fruit wine fermentation yeast was used as the starting strain, and the medium was YEPD medium. The strain was activated by slant passaging, the culture temperature was 26°C, and the culture time was 24 hours. The formula of the YEPD medium is: 10g of yeast extract, 20g of peptone, 20g of glucose, 18g of agar powder, 1000ml of water, pH 5.0, autoclaved at 121°C for 20min;

[0027] 2) Pick the 3-ring activated strain and inoculate it into 50ml YEPD liquid medium, and culture it in a shake flask at 28°C and 150rpm for 20h to obtain the first-grade seed strain. Then, inoculate the primary seed bacterial liquid in 1LYEPD liquid medium at a mass ratio of 5%, and culture again under the same conditions to obtain the secondary seed bacterial liquid;

[0028] 3) The solid fermentation medium is calculated in parts by mass, including 2...

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PUM

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Abstract

The invention discloses blueberry wine fermenting yeast solid preparation and a preparation method thereof. Through production processes easy to massive production, such as liquid culture, solid fermenting and low-temperature drying, the blueberry wine fermenting yeast solid preparation is produced, each gram of preparation contains 1.0-1.5 billion of yeast living bacteria, and contaminating microorganism rate is low. The exclusive yeast agent prepared by the preparation method is low in contaminating microorganism rate (0.1-0.6%) lowered by 10-15% and high in living bacteria rate (90-98%) increased by 10%, and alcoholic strength (11.7-11.8%) generated is increased to 0.5-1.0 V%. Fermenting time (26-28 hours) is shortened to 8-10 hours, and shelf life (2-3 years) is prolonged by 3-6 months. The raw materials are low in cost and obtainable, and the preparation method is mild in reaction condition, simple in reaction device, easy to operate, low in cost, and simple in drying.

Description

technical field [0001] The invention relates to the technical field of preparation methods of yeast inoculum, in particular to a preparation method of a yeast preparation for fermenting blueberry fruit wine. Background technique [0002] Blueberry (Blueberry), whose scientific name is bilberry, belongs to the genus Vaccinium in the family Rhododendron, and is listed as one of the five health foods for human beings by the Food and Agriculture Organization of the United Nations. Blueberry has rich nutritional and health functions, and its anthocyanin content is particularly rich. The blueberry fruit wine processed from blueberries retains various amino acids, vitamins, minerals, sugars, organic acids, etc. needed by the human body in the blueberries, so it also has significant health benefits. Blueberry wine also has significant economic value. The price of ordinary blueberry wine is also 200-500 yuan / 500ml, which is several times the price of other ordinary fruit wines; the...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12G3/02C12R1/865
CPCC12N1/16C12G3/02
Inventor 任春光龙云川王莹刘曼聂飞唐军李永霞
Owner GUIZHOU INST OF BIOLOGY
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