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Immunochromatographic detection method of Norovirus Raman microprobe labeling

An immunochromatographic detection and Raman probe technology, which is applied in the field of norovirus Raman probe-labeled immunochromatographic detection, can solve the problems of complex experimental process, high equipment requirements, and inappropriate online detection, and achieve detection The effect of simple equipment, high sensitivity, and reduced workload

Inactive Publication Date: 2016-07-13
SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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Problems solved by technology

In recent years, there have been reports on the application of SERS technology to study microorganisms. However, the experiments involved in these reports have high requirements for instruments and complicated experimental processes, which are not suitable for online detection.

Method used

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  • Immunochromatographic detection method of Norovirus Raman microprobe labeling
  • Immunochromatographic detection method of Norovirus Raman microprobe labeling
  • Immunochromatographic detection method of Norovirus Raman microprobe labeling

Examples

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Embodiment 1

[0028] Example 1 Norovirus Raman probe-labeled immunochromatographic detection method performance analysis

[0029] 1. Experimental steps

[0030] 1. Sample Preparation

[0031] In this embodiment, the positive sample is the stool of a norovirus-infected person, and the negative control sample is the stool of a healthy person.

[0032] 2. Sample testing process:

[0033] (1) Preparation of Raman-labeled immune colloidal gold-antibody complexes: Raman-labeled immune colloidal gold-antibody complexes, including colloidal gold (1.2*10 -4 Chlorauric acid, 0.7*10 -4 Trisodium citrate aqueous solution), norovirus VP1 protein antibody, probe molecule 4-mercaptobenzoic acid (4-MBA). The preparation method is to add 20μg antibody to 1mL colloidal gold solution, add 1mM 4-MBA solution, vortex mix, shake for 20min, add 1mL PBS solution containing 1% casein, vortex mix and react for 1h After centrifugation at 5000r / min for 10min, about 100μL of the bottom precipitate was collected an...

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Abstract

The invention relates to an immunochromatographic detection method of Norovirus Raman microprobe labeling.A principle of the detection method includes: using nitrocellulose membrane (NC) as an SERS substrate, labeling an immunoprobe by Raman labeling, constructing novel surface enhanced Raman spectroscopy and immunochromatographic assay (SERS-ICA) based on colloidal gold ICA, and detecting Norovirus by using the method.Detection includes sample fixation, colloidal gold penetration, antigen antibody reaction, detection and the like, detection results are used to read peaks under the band of 1074 cm<-1> through software on a detection instrument, some peaks are positive, and no peaks are negative.The method is suitable for quickly and sensitively detecting Norovirus and is simple to perform, it is possible to greatly improve detection efficiency for inspection and quarantine staff on the front line of entry and exit ports through the method, and the method is significant to controlling diarrhea due to Norovirus.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to an immunochromatographic detection method labeled with a norovirus Raman probe. Background technique [0002] Norovirus infectious diarrhea is diarrhea caused by Norovirus genus viruses. It has the characteristics of acute onset, rapid transmission, and wide coverage. It is the main cause of outbreaks of non-bacterial diarrhea. Noroviruses are responsible for 60-90% of all abacterial diarrhea outbreaks in the United States each year. Developed countries such as the Netherlands, the United Kingdom, Japan, and Australia have similar results. In developing countries, norovirus infectious diarrhea is widespread and often causes outbreaks. The detection rate of norovirus is about 15% in children with diarrhea under 5 years old in my country, and the survey of serum antibody level shows that norovirus infection is also very common in the population of my country....

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/56983G01N33/558G01N2469/10
Inventor 张子龙李深伟田桢干李俊王传现
Owner SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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