Method for detecting quality of Lycium ruthenicum Murr fruit or Lycium ruthenicum Murr fruit extracts

Abstract

The invention provides a method for detecting quality of Lycium ruthenicum Murr fruit or Lycium ruthenicum Murr fruit extract.The method is used for detecting content of the fruit or phenol amine A in the fruit extract of Lycium ruthenicum Murr of Lycium L.of Solanaceae and further used for determination by high-performance liquid chromatography, and chromatographic conditions include that octadecyl bonded-phase silica gel serves as filler, methyl alcohol-water serves as a mobile phase, detection wavelength is 320 nm, separation is conducted, and an ultraviolet detector is used for detecting the phenol amine A of the Lycium ruthenicum Murr.The method is used for determining the content of the phenol amine A of the Lycium ruthenicum Murr in the Lycium ruthenicum Murr fruit according to HPLC (high-performance liquid chromatography) for the first time and provides new scientific bases for establishing quality standards of the Lycium ruthenicum Murr fruit.

Description

technical field [0001] The invention relates to a detection method, in particular to a method for detecting the quality of black wolfberry fruit or black wolfberry fruit extract. Background technique [0002] Black wolfberry (scientific name: Lycium ruthenicum Murr) belongs to the genus Lycium ruthenicum Murr. Distributed in Northwest China and Europe and Central Asia. Black wolfberry is more precious and has medicinal value than ordinary red wolfberry. Tibetan medicine is used to treat heart fever, heart disease, irregular menstruation, menopause, etc., and has significant medicinal effects. It is used as a nourishing and strong medicine in the folk. It is recorded in "Four Medical Codes", "Jingzhu Materia Medica" and other Tibetan medicine classics, and is known as "soft gold" because of its value. [0003] In recent years, wild black wolfberry fruit has been highly sought after by the market, and the market supply is tight. The price per kilogram once approached thou...

AI Technical Summary

Problems solved by technology

[0004] At present, there are mainly three methods for identifying black wolfberry fruit. The first is manual identification, that is, using the naked eye to judge the quality of black wolfberry fruit from the appearance. However, because different people have different understandings of quality grading, it is highly subjective. It is easy to cause disputes, so it is not suitable for third-party testing; the second method is to use the colorimetric method to measure the content of anthocyanins. Since blueberries and black currants also contain high levels of anthocyanins, this method also cannot identify adulteration and other issues well. The third method is to use high performance liquid chromatography to measure the anthocyanin content, and although the anthocyanins in the black wolfberry fruit are inconsistent with the blueberry and black currant, the anthocyanins in the black wolfberry fruit will be after acid hydrolysis. Hydrolyzed to produce anthocyanins, and the anthocyanins in blueberries and black currants will also produce anthocyanins after acid hydrolysis

Therefore, this method is also not suitable for the quality detection of black wolfberry fruit extract, and lacks specificity.

Images