Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SSR mark linked with pseudoperonospora cubensis resistance main effect QTL and application of SSR mark

A technology for downy mildew and melon, which is applied in the field of SSR markers linked with the main effect QTL for downy mildew resistance in melon, can solve the problem of no molecular marker-assisted breeding, etc., and achieve the effects of improving the selection accuracy and shortening the breeding cycle.

Active Publication Date: 2016-07-06
XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the QTL mapping of melon resistance to downy mildew and the development and application of SSR molecular markers to detect the differences in the expression of polymorphisms in QTL loci is basically blank, and there is no effective marker for molecular marker-assisted breeding.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SSR mark linked with pseudoperonospora cubensis resistance main effect QTL and application of SSR mark
  • SSR mark linked with pseudoperonospora cubensis resistance main effect QTL and application of SSR mark
  • SSR mark linked with pseudoperonospora cubensis resistance main effect QTL and application of SSR mark

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment one: the SSR marker linked with the main effect QTL of resistance to melon downy mildew

[0065] A kind of SSR marker linked with the main effect QTL of resistance to melon downy mildew, specifically comprises the following steps:

[0066] (1) Taking the melon resource PI390452 as the source of downy mildew resistance, the resistance gene was quantitatively inherited according to the genetic identification of resistance.

[0067] (2) The melon downy mildew-susceptible farm variety "Karaxai" was used as the female parent and the disease-resistant resource PI390452 was used as the male parent to obtain a hybrid F 1 .

[0068] (3) by Hybrid F 1 Self-pollination to obtain F 2 generation group, F 1 Crossbreed with "Karaxai" to get BC S , F 1 BCr was obtained by hybridization with PI390452.

[0069] (4) To PI390452, "Karaxai", F 1 、BC S , BCr and F 2 Carry out disease resistance identification, investigate the disease situation after inoculating the conid...

Embodiment 2

[0089] Embodiment two: the application of the molecular marker linked with the main effect resistance gene of melon downy mildew

[0090] The application of the molecular marker linked with the main effect resistance gene of melon downy mildew, by the molecular marker SSR-pc provided by the above-mentioned embodiment one 283 Be used for the genotype detection of muskmelon variety or line, be used for judging whether this kind or line is resistant to melon downy mildew, comprises the following steps:

[0091] (1) The disease-resistant resource PI390452 was crossed with other melons and propagated to F 2 generation above;

[0092] (2) Genomic DNA is extracted from the single muskmelon plant obtained by step (1), and the SSR-pc 283 This marker is amplified by PCR to detect whether a 283bp specific band appears, and if a specific band occurs, it is predicted that the melon plant is resistant to downy mildew.

Embodiment 3

[0093] Embodiment three: the construction of genetic segregation population and downy mildew inoculation method and disease classification

[0094] 1. Construction of F2 population

[0095] (1) F 1 , F 1 F 2 . By pair of parents, F 1 Generation and F 2 The generation groups were inoculated manually and the incidence of each individual plant was counted.

[0096] (2) Inoculation of downy mildew and disease classification: Spray inoculation with conidia suspension. Collect natural early diseased leaves from the field, brush the sporangia on the back of the leaves with a soft brush, place them in a beaker filled with sterile water, inoculate them on the cotyledons of susceptible varieties by spot grafting, and then put them in the light Propagate in the incubator. After a large black mold layer grows on the back of the cotyledon, use a soft brush to pick up the sporangia on the back of the leaf, put them in a beaker filled with sterile water, stir them evenly, and count th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an SSR mark linked with a pseudoperonospora cubensis resistance main effect QTL and an application of the SSR mark. A high-resistance resource PI390452 and an infected farm variety 'Kelakesai' construct a pseudoperonospora cubensis infection-resistant F2 generation population, and well-known genetic software is used for performing linkage disequilibrium analysis and establishing a genetic linkage graph, the LOD result analysis shows a peak value at b290, and LOD is more than 3.0. According to b290, well-known SSR search software is used for re-searching an SSS site, and well-known PCR primer design software is used for re-designing an SSR primer. According to a phenotype and a genotype, well-known genetic software is finally used for finding the molecular mark SSR-pc283 linked with the pseudoperonospora cubensis resistance main effect QTL, the genetic distance is 0.5CM, and the SSR mark can be used for molecular mark assisted breeding, so that the good technical effects of shortening the breeding cycle and improving the selection accuracy in the process of breeding a pseudoperonospora cubensis resistant strain are achieved.

Description

technical field [0001] The invention relates to the field of molecular genetic breeding. Specifically, the present invention relates to the technical field of SSR markers linked with the major QTL for resistance to downy mildew of melon and its application. Background technique [0002] Melon (Cucumismelo L., 2n=24) is an important economic crop. The planting area and output of melon in my country rank first in the world. Melon downy mildew [Pseudoperonosporacubensis (Berk.etCurt.) Rostov.] under the conditions of high temperature and high humidity, the epidemic is rapid, the damage is serious, and the control is difficult. It has a direct impact on the yield, fruit sugar content and commercial rate of melon. , is a devastating disease. Chemical control of downy mildew is not only inefficient, but also easy to cause environmental pollution, and cultivating disease-resistant varieties is one of the safest, most effective and cost-saving measures to prevent and control downy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张学军杨永李寐华张永兵张红伊鸿平王豪杰
Owner XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com