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A cadmium-contaminated soil remediation fixative based on microbial assembly synthesis and its preparation and application method

A cadmium-contaminated soil and fixative technology, applied in chemical instruments and methods, applications, soil conditioning materials, etc., can solve problems that are not conducive to practical application, eutrophication of water bodies, and easy to flow away with water, etc., to facilitate large-scale treatment , strong adsorption, process cleaning and sustainable effect

Active Publication Date: 2019-04-23
JIANGXI GAIA ENVIRONMENTAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the nano-scale hydroxyapatite powder is dispersed in large-scale use, it is easy to flow away with the water, resulting in eutrophication of the water body
This not only leads to material loss and increases costs, but also introduces secondary pollution, which is not conducive to its practical application

Method used

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  • A cadmium-contaminated soil remediation fixative based on microbial assembly synthesis and its preparation and application method
  • A cadmium-contaminated soil remediation fixative based on microbial assembly synthesis and its preparation and application method
  • A cadmium-contaminated soil remediation fixative based on microbial assembly synthesis and its preparation and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of the present invention repairs cadmium-contaminated soil fixative:

[0036] (1) Aspergillus niger (Aspergillus niger) was activated and expanded by PDA medium, which was purchased from China Center for Type Culture Collection, strain number CCTCC AF 91006;

[0037] (2) Add 1.0 g of hydroxyapatite nanoparticles (particle size97%) into 100mL of liquid PDA medium, and disperse for 0.5h with an ultrasonic wave with a power of 100 watts;

[0038] (3) Put the PDA medium containing nano-hydroxyapatite into a high-temperature and high-pressure sterilizer, set at 115° C., and sterilize for 25 minutes. After high-temperature and high-pressure sterilization, the culture medium was transferred to an ultra-clean aseptic operating table, and sterilized with an ultraviolet lamp for 30 minutes;

[0039] (4) In the ultra-clean aseptic operation bench, inoculate the activated mycelium in step (1) into the sterilized medium of step (3), the inoculum size is 1 mL; the mass con...

Embodiment 2

[0043] Preparation of the present invention repairs cadmium-contaminated soil fixative:

[0044] (1) The improved LB medium (per 100mL medium: LB medium 2.5g, glucose 9g, sodium nitrate 0.3g) was used to activate and expand the culture of Penicillium Chrysogenum F1, the strain was collected in Zhuzhou, Hunan Province City, preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation number: CGMCC No.5599, and has applied for a patent;

[0045] (2) Add 1.0 g of hydroxyapatite nanoparticles (particle size 97%) into 200 mL of liquid modified LB medium, and disperse for 1.5 h with ultrasonic waves with a power of 100 watts;

[0046] (3) Put the modified LB medium containing nano-hydroxyapatite into a high-temperature and high-pressure sterilizer, set at 115° C., and sterilize for 25 minutes. After high-temperature and high-pressure sterilization, the culture medium was transferred to an ultra-clean aseptic operating ...

Embodiment 3

[0051] The soil was taken from low-pollution paddy soil around a lead-zinc tailings pond. The soil to be tested was air-dried after removing the gravel and broken roots, mixed in quarters, and crushed through a 18-mesh nylon sieve. The pH of the soil is 6.58, which is slightly acidic. The available Cd content in the soil is 7.01mg / kg.

[0052] Add 0.2g of the fixative in Example 1 (the amount of n-HAP contained in the fixative is equal to the amount of pure n-HAP weighed) and pure n-HAP into 5.0g of low-pollution soil, mix well, add 10mL After 7 days of fixation, 25mL DTPA was used to extract the available state of cadmium in the soil. After testing, the available content of cadmium in low-pollution soil before and after treatment with fixative or pure n-HAP is shown in Table 1 below.

[0053] Table 1 Available Cd content in low-polluted soil before and after treatment

[0054]

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Abstract

The invention discloses a cadmium-contaminated soil remediation fixing agent based on microorganism assembly synthesis as well as preparation and an application method thereof. At present, multiple nano phosphate fixing agents for cadmium-contaminated soil remediation are easily dispersed and lost, underground water eutrophication is caused, and further secondary pollution is caused. Aiming at solving the problems, nano-hydroxyapatite is taken as a central substance, aspergillus niger hyphae are adopted for wrapping the nano-hydroxyapatite, and a cadmium-contaminated soil remediation fixing agent specific to high contamination and high acidity is prepared, so that the problem of excessive dispersion in practical application is solved. A synthesized product is uniformly mixed with cadmium-contaminated soil in a certain rate, and effective state cadmium content is reduced by 67-86%. The cadmium-contaminated soil remediation fixing agent disclosed by the invention is simple in preparation process, low in cost, efficient, non-toxic and biodegradable and has no secondary pollution, thereby being an environment-friendly fixing agent.

Description

technical field [0001] The invention relates to the technical field of combination of micro-nano material assembly and cadmium-contaminated soil remediation, in particular to a fixative product suitable for cadmium-contaminated soil remediation and its preparation and application method. Background technique [0002] In recent years, soil pollution cases such as "cadmium rice", "heavy metal vegetables" and "blood lead incident" have been frequently reported in the newspapers, arousing great public concern. According to the "National Soil Pollution Survey Bulletin" jointly issued by the Ministry of Environmental Protection and the Ministry of Land and Resources in April 2014, the overall situation of the national soil environment is not optimistic. Soil environment problems are prominent. Inorganic pollutants are the main type of pollution, followed by organic pollutants. Inorganic pollutants exceeded the standard number and accounted for 82.8% of the total. The "five envir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/40
CPCC09K17/40
Inventor 杨志辉粱丽芬柴立元侍维王海鹰廖骐杨卫春
Owner JIANGXI GAIA ENVIRONMENTAL SCI & TECH CO LTD
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